FastQCFastQC Report
Thu 26 May 2016
SRR1546138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546138_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences441910
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240055.432101559141002No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68951.5602724536670363No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT45631.0325631916000995No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCGAGCTCCATCGTATG42430.960150256839628No Hit
GAATGATACCTGTCCCTTATACACATCTGACGCGAGCTCCATCGTATGCC25800.5838292865063022No Hit
GAATCTTTCTCTTACCCACATCTGACGCGAGCTCCATCGTATGCCGTCTT24910.5636894390260461No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC24040.5440021723880428No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT23200.5249937770134191No Hit
GAACTGTCCCTTATCCACATCTGACGCGAGCTCCATCGTATGCCGTCTTC17960.4064175963431468No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATG15970.3613858025389785No Hit
CGTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12830.29033061030526575No Hit
GAACTGTCCCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC9930.2247063881785884No Hit
GAATCTGTCTCTTACCCACATCTGACGCGAGCTCCATCGTATGCCGTCTT9320.21090267248987352No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTCCA8920.20185105564481456No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8150.1844266932180761No Hit
GCTGTCTCCTATACCCATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG7850.17763798058428187No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7840.1774116901631554No Hit
GAATGACTCTCTCTCCTACACATCTGACGCGAGCTCCATCGTATGCCGTC7180.16247652236880814No Hit
GAATGCTGCCTCTTCCACACATCTGACGCGAGCTCCATCGTATGCCGTCT6970.15772442352515217No Hit
GAATCTTTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT6810.1541037767871286No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCCT6750.15274603426036976No Hit
GAATGATACCTGGCCCTTATACACATCTGACGCGAGCTCCATCGTATGCC6330.14324183657305786No Hit
GAATGATCCGTCTCCCATACACATCTGACGCGAGCTCCATCGTATGCCGT6270.14188409404629904No Hit
GAATGATACGGCGACCGTCTCTTATACACATCTGACGCGAGCTCCATCGT5720.12943812088434295No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT5110.11563440519562808No Hit
GAATGACTCTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC4970.11246633929985744No Hit
CGTTTTTTCTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.10997714466746623No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT4850.10975085424633976No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4770.10794053087732795No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGCTCCATCG4440.10047294698015434No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTG302.5241661E-644.00000429
GCGGAAT302.5241661E-644.0000048
CGGGTAC408.292773E-944.06
ATAGGCG351.4433863E-744.04
GACCGCC408.292773E-944.013
CACGACG502.7284841E-1144.026
TACGGGA351.4433863E-744.04
TGCGGGT207.851217E-444.04
ATCCGTC1250.044.06
GTAATCA207.851217E-444.030
CTAACGC408.292773E-944.024
GCGATTT207.851217E-444.08
CGTAAGG254.4383785E-544.02
TAGGCAA207.851217E-444.05
TCATACG207.851217E-444.026
AATAGCG207.851217E-444.01
GCGTAGG502.7284841E-1144.02
TGCGTAG207.851217E-444.01
CGTTTTT137850.043.5850561
TGATCCG1650.042.6666684
Produced by FastQC (version 0.11.5)