##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546138_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	441910
Sequences flagged as poor quality	0
Sequence length	50
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.22597587744111	28.0	16.0	31.0	16.0	33.0
2	25.19967414179358	30.0	16.0	31.0	16.0	33.0
3	25.563370369532258	30.0	16.0	31.0	16.0	33.0
4	25.949319997284515	28.0	19.0	33.0	10.0	35.0
5	31.26356950510285	33.0	32.0	35.0	25.0	35.0
6	31.088778258016337	35.0	30.0	35.0	17.0	35.0
7	30.52383290715304	33.0	28.0	35.0	17.0	35.0
8	31.546799121993168	35.0	31.0	35.0	25.0	35.0
9	26.517564662487835	28.0	17.0	34.0	11.0	37.0
10	29.8145436853658	33.0	25.0	37.0	17.0	39.0
11	32.09509176076577	35.0	30.0	38.0	17.0	39.0
12	32.27752257246951	35.0	28.0	39.0	17.0	39.0
13	32.69406213934964	35.0	31.0	39.0	17.0	39.0
14	33.97035369192822	36.0	31.0	40.0	25.0	41.0
15	31.651716412844245	34.0	27.0	39.0	17.0	41.0
16	31.02281007444955	34.0	27.0	39.0	17.0	41.0
17	31.304964811839515	34.0	27.0	39.0	17.0	41.0
18	31.724183657305787	35.0	27.0	39.0	17.0	39.0
19	30.98151207259397	35.0	27.0	37.0	16.0	39.0
20	30.877441107917903	34.0	27.0	35.0	18.0	39.0
21	32.101280803783574	34.0	30.0	36.0	22.0	39.0
22	32.64113054694395	35.0	31.0	37.0	25.0	39.0
23	33.01069674820665	35.0	32.0	37.0	24.0	39.0
24	33.12812563644181	35.0	32.0	37.0	24.0	39.0
25	32.30989341721165	35.0	31.0	37.0	21.0	39.0
26	25.446351066959334	32.0	16.0	35.0	0.0	38.0
27	28.758984861170827	33.0	21.0	36.0	11.0	39.0
28	30.295822678826006	33.0	25.0	36.0	17.0	39.0
29	30.930336493856213	33.0	28.0	36.0	18.0	38.0
30	30.94763187074291	33.0	29.0	36.0	18.0	39.0
31	30.022801022832702	33.0	26.0	35.0	15.0	38.0
32	30.038799755606345	33.0	25.0	36.0	15.0	39.0
33	29.365739630241453	33.0	25.0	36.0	12.0	38.0
34	28.52408182661628	33.0	22.0	36.0	8.0	38.0
35	27.296030866013442	33.0	18.0	35.0	7.0	38.0
36	27.19386074087484	33.0	18.0	35.0	7.0	38.0
37	27.107241293476047	33.0	18.0	36.0	7.0	38.0
38	26.83054015523523	32.0	18.0	36.0	7.0	38.0
39	26.59588830304813	32.0	17.0	36.0	7.0	38.0
40	26.254723812541016	31.0	16.0	35.0	7.0	38.0
41	25.080195062343012	30.0	14.0	35.0	7.0	38.0
42	25.482028014754135	31.0	15.0	35.0	7.0	38.0
43	25.31129415491842	30.0	15.0	35.0	7.0	38.0
44	25.3569708764228	31.0	15.0	35.0	7.0	38.0
45	25.203034554547305	30.0	15.0	35.0	7.0	37.0
46	24.25703650064493	28.0	12.0	34.0	7.0	37.0
47	24.140429046638456	28.0	12.0	34.0	7.0	37.0
48	24.332612975492747	28.0	13.0	34.0	7.0	36.0
49	24.104638953633092	28.0	12.0	34.0	7.0	36.0
50	24.9502613654364	28.0	13.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	8.0
12	27.0
13	96.0
14	386.0
15	1005.0
16	2105.0
17	3497.0
18	4963.0
19	6740.0
20	8447.0
21	11145.0
22	14063.0
23	18087.0
24	23242.0
25	28674.0
26	32298.0
27	32625.0
28	31393.0
29	30151.0
30	30708.0
31	32169.0
32	33410.0
33	33715.0
34	30144.0
35	22232.0
36	8831.0
37	1709.0
38	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.761919847932838	16.67013645312394	13.117829422280556	39.45011427666267
2	49.141680432667286	28.4494580344414	14.02050191215406	8.388359620737255
3	18.157317100767127	26.004390034169855	47.06026113914598	8.778031725917042
4	16.751827295150594	16.570342377407165	56.642076440904255	10.035753886537984
5	21.19345568102102	19.184901903102443	46.64230273132539	12.979339684551153
6	11.643773619062706	33.09090086216651	48.26729424543459	6.9980312733362
7	41.42992917109819	7.592948790477699	48.27182005385712	2.7053019845669937
8	40.32427417347424	15.530085311488765	39.334253581045914	4.811386933991084
9	31.72636962277387	6.927881242786993	33.450928922178726	27.894820212260413
10	22.979792265393407	19.28446968839809	45.154669502839944	12.58106854336856
11	20.334004661582675	19.24984725396574	47.12995858885293	13.286189495598652
12	14.734221900386956	17.13787875359236	51.84358806091738	16.2843112851033
13	15.290896336358081	20.421126473716367	54.066212577221606	10.221764612703945
14	11.89178792061732	28.046434794415156	48.04552963273065	12.016247652236881
15	8.827815618564866	19.04256522821389	49.357561494421944	22.7720576587993
16	9.586114819759679	17.577108460998847	46.348125183860965	26.48865153538051
17	10.150709420470232	20.942273313570638	50.373831775700936	18.533185490258198
18	10.374284356543187	22.66886922676563	48.532054038152566	18.424792378538616
19	12.607544522640357	23.65210110656016	45.83987689801091	17.900477472788577
20	13.299314340023988	23.004457921296193	49.99026951189156	13.705958226788258
21	13.879522979792267	24.669729130365912	48.50603063972302	12.944717250118803
22	10.930053630829807	18.74024122558892	50.752641940666656	19.577063202914623
23	12.45592994048562	25.450204792831123	48.69543572220588	13.398429544477382
24	14.513136158946391	23.085017311217214	47.4852345500215	14.916611979814896
25	13.834038605145842	26.66402661175352	45.29655359688625	14.205381186214389
26	10.29327238577991	33.74985856848679	39.71396890769614	16.242900138037157
27	14.76929691566156	22.443257676902537	46.87696589803353	15.910479509402368
28	13.288452399809916	22.519517548822158	49.74067117738906	14.451358873978865
29	17.495643909393316	21.886356950510287	46.45900749021294	14.15899164988346
30	13.59100269285601	26.04059650155009	48.80609173813673	11.562309067457175
31	19.950216107352176	19.938675295874724	42.39234233214908	17.71876626462402
32	16.091059265461293	23.716820167002332	42.791066054173925	17.40105451336245
33	13.827476182933177	20.455522617727592	44.01439207078364	21.70260912855559
34	16.595460614152202	22.223303387567604	39.686135185897584	21.49510081238261
35	13.120771197755198	25.14765449978502	38.41845624674708	23.3131180557127
36	19.699486320744043	25.663370369532256	36.22977529361182	18.407368016111878
37	12.789255730804916	26.093095879251432	38.496752732456834	22.62089565748682
38	17.72555497725781	26.15939897264149	35.70274490280826	20.412301147292435
39	16.22977529361182	23.3823629245774	35.23862324907787	25.14923853273291
40	14.129800185558144	23.427168427960442	41.8001403000611	20.642891086420313
41	14.964585549093707	26.255798692041367	36.77513520852662	22.004480550338304
42	15.818153017582764	24.98110474983594	36.954809802901046	22.245932429680252
43	18.306895069131723	25.43934285261705	34.07503790364554	22.178724174605687
44	16.114819759679573	23.87793894684438	36.86361476318707	23.143626530288973
45	16.981964653436222	23.262881582222626	35.1363399787287	24.618813785612456
46	20.17899572311104	23.307913376026793	34.546174560430856	21.966916340431307
47	14.527392455477358	23.386662442578807	40.93186395419882	21.154081147745014
48	15.23771808739336	26.04693263334163	34.52331922789708	24.192030051367926
49	14.258333144757982	23.370143241836573	38.83822497793668	23.53329863546876
50	15.676721504378719	22.152248195333893	39.6888506709511	22.48217962933629
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8223.0
1	7405.5
2	6588.0
3	22546.0
4	38504.0
5	28035.0
6	17566.0
7	15368.5
8	13171.0
9	13038.5
10	12906.0
11	12650.0
12	12394.0
13	11957.0
14	11520.0
15	11091.0
16	10662.0
17	10048.0
18	9434.0
19	8756.0
20	8078.0
21	7582.0
22	7086.0
23	6474.5
24	5863.0
25	5399.0
26	4935.0
27	4822.5
28	4710.0
29	4833.0
30	4956.0
31	5189.0
32	5422.0
33	5585.5
34	5749.0
35	6312.5
36	6876.0
37	7161.5
38	7447.0
39	8092.0
40	8737.0
41	9789.5
42	10842.0
43	12042.5
44	13243.0
45	15676.0
46	18109.0
47	23505.0
48	28901.0
49	32979.0
50	37057.0
51	33253.0
52	29449.0
53	26098.5
54	22748.0
55	19752.5
56	16757.0
57	14511.0
58	12265.0
59	11142.5
60	10020.0
61	8722.5
62	7425.0
63	6713.0
64	6001.0
65	5316.0
66	4631.0
67	4167.0
68	3703.0
69	3509.0
70	3315.0
71	2981.0
72	2647.0
73	2199.0
74	1751.0
75	1334.0
76	917.0
77	697.5
78	478.0
79	413.5
80	349.0
81	290.5
82	232.0
83	168.5
84	105.0
85	88.5
86	72.0
87	57.5
88	43.0
89	28.0
90	13.0
91	8.5
92	4.0
93	2.5
94	1.0
95	3.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	441910.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.518585808884026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.24286001862602	47.01838764329035
2	6.470030070853706	7.0547377920779555
3	2.5054768234745244	4.097851595782981
4	1.3552072257230918	2.9553592569761613
5	0.8481882666183653	2.312101239786097
6	0.5767109780802167	1.8864880167235032
7	0.39377094116695815	1.5027484391539079
8	0.3084720579275614	1.345396828781344
9	0.2568226090225386	1.2601444902891068
>10	0.9367973737403396	8.164703668848585
>50	0.04972406341963092	1.872447423503482
>100	0.04484045004802695	4.91977331051063
>500	0.00621550792744928	2.496677245789489
>1k	0.003995683667645965	5.634043262262911
>5k	4.439648519606629E-4	1.6688889587705793
>10k+	4.439648519606629E-4	5.810250827452902
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24005	5.432101559141002	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6895	1.5602724536670363	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT	4563	1.0325631916000995	No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCGAGCTCCATCGTATG	4243	0.960150256839628	No Hit
GAATGATACCTGTCCCTTATACACATCTGACGCGAGCTCCATCGTATGCC	2580	0.5838292865063022	No Hit
GAATCTTTCTCTTACCCACATCTGACGCGAGCTCCATCGTATGCCGTCTT	2491	0.5636894390260461	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC	2404	0.5440021723880428	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT	2320	0.5249937770134191	No Hit
GAACTGTCCCTTATCCACATCTGACGCGAGCTCCATCGTATGCCGTCTTC	1796	0.4064175963431468	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATG	1597	0.3613858025389785	No Hit
CGTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1283	0.29033061030526575	No Hit
GAACTGTCCCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC	993	0.2247063881785884	No Hit
GAATCTGTCTCTTACCCACATCTGACGCGAGCTCCATCGTATGCCGTCTT	932	0.21090267248987352	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTCCA	892	0.20185105564481456	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	815	0.1844266932180761	No Hit
GCTGTCTCCTATACCCATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG	785	0.17763798058428187	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	784	0.1774116901631554	No Hit
GAATGACTCTCTCTCCTACACATCTGACGCGAGCTCCATCGTATGCCGTC	718	0.16247652236880814	No Hit
GAATGCTGCCTCTTCCACACATCTGACGCGAGCTCCATCGTATGCCGTCT	697	0.15772442352515217	No Hit
GAATCTTTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT	681	0.1541037767871286	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCCT	675	0.15274603426036976	No Hit
GAATGATACCTGGCCCTTATACACATCTGACGCGAGCTCCATCGTATGCC	633	0.14324183657305786	No Hit
GAATGATCCGTCTCCCATACACATCTGACGCGAGCTCCATCGTATGCCGT	627	0.14188409404629904	No Hit
GAATGATACGGCGACCGTCTCTTATACACATCTGACGCGAGCTCCATCGT	572	0.12943812088434295	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT	511	0.11563440519562808	No Hit
GAATGACTCTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC	497	0.11246633929985744	No Hit
CGTTTTTTCTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	486	0.10997714466746623	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	485	0.10975085424633976	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	477	0.10794053087732795	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGCTCCATCG	444	0.10047294698015434	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.077391324025254	0.0
2	0.0	0.0	0.0	0.37066370980516394	0.0
3	0.0	0.0	0.0	0.6854336855920888	0.0
4	0.0	0.0	0.0	0.8961100676608359	0.0
5	0.0	0.0	0.0	2.4699599465954605	0.0
6	0.0	0.0	0.0	2.6623068045529634	0.0
7	0.0	0.0	0.0	2.8272725215541628	0.0
8	0.0	0.0	0.0	3.0370437419384038	0.0
9	0.0	0.0	0.0	3.216718336312824	0.0
10	0.0	0.0	0.0	4.456563553664774	0.0
11	0.0	0.0	0.0	4.6310334683532846	0.0
12	0.0	0.0	0.0	5.488221583580367	0.0
13	0.0	0.0	0.0	5.556108709918309	0.0
14	0.0	0.0	0.0	5.604082279197121	0.0
15	0.0	0.0	0.0	5.703423774071643	0.0
16	0.0	0.0	0.0	5.884229820551696	0.0
17	0.0	0.0	0.0	5.953474689416397	0.0
18	0.0	0.0	0.0	6.005974067117739	0.0
19	0.0	0.0	0.0	6.3598922857595435	0.0
20	0.0	0.0	0.0	6.410807630513	0.0
21	0.0	0.0	0.0	6.442488289470707	0.0
22	0.0	0.0	0.0	6.514901224231179	0.0
23	0.0	4.525808422529474E-4	0.0	6.550428820348035	0.0
24	0.0	4.525808422529474E-4	0.0	6.596365775836709	0.0
25	0.0	4.525808422529474E-4	0.0	6.621710303002874	0.0
26	0.0	4.525808422529474E-4	0.0	6.6667420968070426	0.0
27	0.0	4.525808422529474E-4	0.0	6.733950351881605	0.0
28	0.0	4.525808422529474E-4	0.0	6.779434726528026	0.0
29	0.0	4.525808422529474E-4	0.0	6.821072164015297	0.0
30	0.0	4.525808422529474E-4	0.0	6.860899278133557	0.0
31	0.0	4.525808422529474E-4	0.0	6.896426874250413	0.0
32	0.0	4.525808422529474E-4	0.0	6.934896245841913	0.0
33	0.0	4.525808422529474E-4	0.0	6.980833201330587	0.0
34	0.0	4.525808422529474E-4	0.0	7.027222737661515	0.0
35	0.0	4.525808422529474E-4	0.0	7.089905184313548	0.0
36	0.0	4.525808422529474E-4	0.0	7.1267905229571635	0.0
37	0.0	4.525808422529474E-4	0.0	7.164807313706411	0.0
38	0.0	4.525808422529474E-4	0.0	7.218211853092258	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGTG	30	2.5241661E-6	44.000004	29
GCGGAAT	30	2.5241661E-6	44.000004	8
CGGGTAC	40	8.292773E-9	44.0	6
ATAGGCG	35	1.4433863E-7	44.0	4
GACCGCC	40	8.292773E-9	44.0	13
CACGACG	50	2.7284841E-11	44.0	26
TACGGGA	35	1.4433863E-7	44.0	4
TGCGGGT	20	7.851217E-4	44.0	4
ATCCGTC	125	0.0	44.0	6
GTAATCA	20	7.851217E-4	44.0	30
CTAACGC	40	8.292773E-9	44.0	24
GCGATTT	20	7.851217E-4	44.0	8
CGTAAGG	25	4.4383785E-5	44.0	2
TAGGCAA	20	7.851217E-4	44.0	5
TCATACG	20	7.851217E-4	44.0	26
AATAGCG	20	7.851217E-4	44.0	1
GCGTAGG	50	2.7284841E-11	44.0	2
TGCGTAG	20	7.851217E-4	44.0	1
CGTTTTT	13785	0.0	43.585056	1
TGATCCG	165	0.0	42.666668	4
>>END_MODULE