FastQCFastQC Report
Thu 26 May 2016
SRR1546137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546137_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1054666
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230012.1808800131984913No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT32480.3079647964379244No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT30170.28606212772574446No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCC28020.2656765269763129No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT27850.2640646422658927No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT25460.24140343957233854No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATG17030.16147292128503243No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT14220.1348294151892637No Hit
GGGCAGGGGCTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14000.13274344674048466No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT11510.10913407657021275No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATC207.856187E-444.032
TCGTTAA254.4425895E-544.035
GATCGTA207.856187E-444.09
CGAGTTA207.856187E-444.032
ACGATTG551.8189894E-1244.01
GTATGCG254.4425895E-544.01
TACGACA207.856187E-444.042
CGTCGCA254.4425895E-544.036
TCGAGTA207.856187E-444.021
GACGATT207.856187E-444.044
CGCAACA254.4425895E-544.037
CGTTTTT136450.043.322831
GCGTAAG700.040.8571431
CGTTGAT650.040.61538325
TACGGGA1700.040.117654
TCACGAC2800.040.0714325
CGGTCTA2800.040.0714331
GATCGCT557.8216544E-1140.010
CGCATCG557.8216544E-1140.021
TAAGGGC3650.039.780824