##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546137_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1054666
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.83238200529836	31.0	28.0	31.0	16.0	33.0
2	28.852758124373025	31.0	28.0	33.0	16.0	33.0
3	28.341074804724908	31.0	28.0	33.0	16.0	34.0
4	32.34081595500376	35.0	32.0	35.0	26.0	37.0
5	33.73329471131145	35.0	33.0	35.0	30.0	37.0
6	33.46120098685271	35.0	33.0	37.0	28.0	37.0
7	34.33791740702744	35.0	35.0	37.0	31.0	37.0
8	34.70189804165489	35.0	35.0	37.0	32.0	37.0
9	36.446267349094406	39.0	36.0	39.0	32.0	39.0
10	36.08648140738395	37.0	35.0	39.0	31.0	39.0
11	36.279039051225695	38.0	35.0	39.0	31.0	39.0
12	35.14326241672719	37.0	34.0	39.0	29.0	39.0
13	35.54910369728426	37.0	35.0	39.0	30.0	39.0
14	36.30236681565538	38.0	35.0	40.0	30.0	41.0
15	36.72949255972981	38.0	35.0	40.0	31.0	41.0
16	36.33150210587996	38.0	34.0	40.0	31.0	41.0
17	35.74540565449156	37.0	34.0	40.0	29.0	41.0
18	35.5007860308382	37.0	34.0	39.0	29.0	40.0
19	34.97326736616142	37.0	33.0	39.0	27.0	40.0
20	35.255278922426626	36.0	34.0	39.0	29.0	40.0
21	35.58529050903319	36.0	34.0	39.0	30.0	40.0
22	35.89004575856243	37.0	34.0	40.0	31.0	41.0
23	36.22752226771319	37.0	35.0	40.0	31.0	41.0
24	35.9627815820364	37.0	35.0	40.0	31.0	41.0
25	35.67815592803788	37.0	34.0	40.0	30.0	41.0
26	35.73466007247792	37.0	34.0	40.0	30.0	41.0
27	36.01906859612427	37.0	35.0	40.0	31.0	41.0
28	35.927314429402294	37.0	35.0	40.0	31.0	41.0
29	35.96798512514862	37.0	35.0	40.0	31.0	41.0
30	35.59539228533014	37.0	35.0	40.0	30.0	41.0
31	34.93731380361176	36.0	34.0	40.0	27.0	41.0
32	34.84141424868157	37.0	34.0	40.0	26.0	41.0
33	34.48682616107848	37.0	34.0	40.0	23.0	41.0
34	34.13819351339666	37.0	34.0	40.0	21.0	41.0
35	33.84421229090537	37.0	33.0	40.0	18.0	41.0
36	33.4918467078677	36.0	33.0	40.0	18.0	41.0
37	33.422016069542394	36.0	33.0	40.0	18.0	41.0
38	33.22440374488227	36.0	33.0	40.0	16.0	41.0
39	33.07122823718599	36.0	33.0	40.0	15.0	41.0
40	32.85318574790502	36.0	32.0	40.0	15.0	40.0
41	32.51486536969998	35.0	32.0	39.0	14.0	40.0
42	32.724819042237066	36.0	32.0	40.0	15.0	40.0
43	32.51575380262567	35.0	32.0	39.0	13.0	40.0
44	32.36054352752436	35.0	31.0	39.0	12.0	40.0
45	32.235690730525114	35.0	31.0	39.0	12.0	40.0
46	32.06110939387446	35.0	31.0	39.0	12.0	40.0
47	31.992912448111536	35.0	31.0	39.0	12.0	40.0
48	31.950606163467867	35.0	31.0	38.0	12.0	40.0
49	31.917412716442932	35.0	31.0	38.0	12.0	40.0
50	32.15266918626371	35.0	31.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	7.0
12	8.0
13	21.0
14	50.0
15	118.0
16	346.0
17	653.0
18	1373.0
19	2284.0
20	3147.0
21	4895.0
22	7030.0
23	9702.0
24	14075.0
25	21375.0
26	29691.0
27	33713.0
28	32224.0
29	31380.0
30	33882.0
31	40507.0
32	53016.0
33	77394.0
34	115663.0
35	98484.0
36	107011.0
37	146272.0
38	157181.0
39	33161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.608078766168628	21.6210629715948	24.764712240652493	23.006146021584083
2	41.40031061966537	20.002635905585276	26.252292194874965	12.344761279874387
3	30.62372352953447	18.925043568295553	37.66955604902405	12.781676853145923
4	27.142242188522243	22.89653786127551	35.63241822529597	14.328801724906274
5	20.058008886225593	27.65539042692189	36.024390660171086	16.262210026681434
6	18.188507072381206	38.177015282563396	34.05248675884119	9.581990886214214
7	74.7243203061443	4.668112938124487	18.091604356260653	2.5159623994705433
8	75.35124864175009	4.396273322549509	17.25721697674904	2.995261058951365
9	71.84151190993168	4.941754071905229	18.373020463350482	4.843713554812614
10	38.09168020965879	24.340691745064312	24.952354584294934	12.61527346098196
11	33.19799822882315	21.362213250450854	29.20147231445785	16.23831620626815
12	29.3608592672941	19.44615641349963	35.67328424354251	15.519700075663764
13	20.67061989293293	27.49448640612289	35.99329076693474	15.84160293400944
14	16.47791812763472	33.46528664050989	35.380869393722755	14.675925838132642
15	15.942298320036866	23.767240055145418	44.09642483971229	16.194036785105425
16	18.043532265191065	17.647198259923048	45.260490050878666	19.04877942400722
17	17.072419135536748	17.919132692245697	36.08877123184022	28.919676940377332
18	20.085031659312047	23.41840924046096	38.120220050708	18.376339049518993
19	23.72277100048736	25.607727944202242	32.00548799335524	18.66401306195516
20	24.742619938444967	22.6029852104837	32.102675159718814	20.551719691352524
21	22.373244230874988	24.039933021449443	35.22849888021421	18.35832386746136
22	19.56429808109866	19.716099694121173	34.166172039299646	26.55343018548052
23	17.93013143497562	27.279536839150975	32.66010281928117	22.13022890659223
24	19.353141184033618	25.052101802845637	39.01197156256104	16.582785450559705
25	23.29704380344109	21.05472253775129	36.05966249030499	19.588571168502636
26	16.722640153375572	25.811963218687247	33.182069015214296	24.28332761272289
27	17.954688972622613	24.496665294984385	37.46930307794126	20.07934265445174
28	16.8555732336114	24.436172209969794	41.377459783476475	17.330794772942333
29	18.68487274644295	25.177070276277043	37.6939239531757	18.444133024104314
30	20.118596787987855	23.122296537482008	40.24051216214423	16.518594512385913
31	27.474480072364145	23.16145585427045	31.461713945457614	17.90235012790779
32	24.91916872260981	25.492430779033366	31.90763710975797	17.680763388598855
33	23.239110770613635	23.25428145024112	30.19316067835694	23.313447100788306
34	19.189392660804465	25.81234248567793	31.730803875350112	23.267460978167495
35	19.92109350258755	23.7773854471463	33.055299023577135	23.24622202668902
36	28.23329850398136	24.339648760839925	28.63200292794117	18.79504980723755
37	19.623937815384206	27.87432229729602	30.968856491059725	21.532883396260047
38	21.38440036940605	30.92457706989701	26.764207815554876	20.926814745142064
39	19.55462677283614	25.32849262230886	29.405612772195177	25.711267832659818
40	21.13067075263638	23.513320804880408	33.85801760936638	21.497990833116834
41	18.699474525584403	22.619862591569273	34.188074708011825	24.4925881748345
42	22.065563884680078	26.916009428577386	27.589113520299318	23.429313166443215
43	20.720777952451296	23.866323556462422	29.66313505887172	25.749763432214557
44	20.305480597648923	25.18579341706284	32.648629992812886	21.860095992475344
45	21.54691627491547	28.233488137476698	29.207825036551853	21.011770551055974
46	24.47030623913163	25.17327760637017	29.97878001187106	20.377636142627146
47	22.319672768440434	26.606906831167404	29.720119924222455	21.353300476169707
48	20.40826195212513	28.528652672978932	30.245404706324088	20.81768066857185
49	20.37517090718768	26.613354370009084	31.772049160587336	21.239425562215906
50	19.90459538849266	25.654188150561406	33.432385229067776	21.00883123187815
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	820.0
1	1308.5
2	1797.0
3	18392.5
4	34988.0
5	24113.5
6	13239.0
7	13289.0
8	13339.0
9	13750.0
10	14161.0
11	13843.5
12	13526.0
13	12850.0
14	12174.0
15	11289.5
16	10405.0
17	9589.0
18	8773.0
19	8021.0
20	7269.0
21	6827.0
22	6385.0
23	6280.0
24	6175.0
25	6369.0
26	6563.0
27	7657.5
28	8752.0
29	10189.5
30	11627.0
31	12361.5
32	13096.0
33	16105.0
34	19114.0
35	20302.5
36	21491.0
37	23906.5
38	26322.0
39	32147.0
40	37972.0
41	46853.0
42	55734.0
43	68429.5
44	81125.0
45	84143.5
46	87162.0
47	89114.0
48	91066.0
49	93330.5
50	95595.0
51	85673.5
52	75752.0
53	65503.0
54	55254.0
55	50530.5
56	45807.0
57	44203.0
58	42599.0
59	39207.5
60	35816.0
61	32224.0
62	28632.0
63	25828.5
64	23025.0
65	18942.0
66	14859.0
67	12838.0
68	10817.0
69	9752.0
70	8687.0
71	7813.0
72	6939.0
73	5443.0
74	3947.0
75	2964.0
76	1981.0
77	1402.5
78	824.0
79	665.0
80	506.0
81	408.0
82	310.0
83	214.5
84	119.0
85	85.5
86	52.0
87	40.0
88	28.0
89	25.0
90	22.0
91	14.5
92	7.0
93	8.0
94	9.0
95	6.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1054666.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.26377717807652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.40954196018738	21.87886045419701
2	7.311635885323034	4.133088949399969
3	3.151464291630207	2.672168535699028
4	1.98186556640184	2.240600270627361
5	1.4208101087532043	2.0078730163079617
6	1.0331218091906247	1.7519954767665065
7	0.8373919324929745	1.6567501293490228
8	0.6486495722730193	1.4666629581903408
9	0.5435852755891603	1.3827395795881805
>10	4.500927658715086	27.608281366524213
>50	0.9006816606292138	17.997318869444694
>100	0.24885921990272655	9.50721991495444
>500	0.0077557751460183575	1.4928983864928689
>1k	0.0033720761504427645	2.0113717791461636
>5k	0.0	0.0
>10k+	3.372076150442764E-4	2.1921703133122112
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23001	2.1808800131984913	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3248	0.3079647964379244	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3017	0.28606212772574446	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCC	2802	0.2656765269763129	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2785	0.2640646422658927	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT	2546	0.24140343957233854	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATG	1703	0.16147292128503243	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1422	0.1348294151892637	No Hit
GGGCAGGGGCTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1400	0.13274344674048466	No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1151	0.10913407657021275	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04143491873256557	0.0
2	0.0	0.0	0.0	0.19864108637236813	0.0
3	0.0	0.0	0.0	0.25704820293818137	0.0
4	0.0	0.0	0.0	0.42240861087775655	0.0
5	0.0	0.0	0.0	0.8935530300588054	0.0
6	0.0	0.0	0.0	1.1051840108622066	0.0
7	0.0	0.0	0.0	1.2719666700168584	0.0
8	0.0	0.0	0.0	1.565140053817986	0.0
9	0.0	0.0	0.0	1.6584397335270122	0.0
10	0.0	0.0	0.0	2.105690332294774	0.0
11	0.0	0.0	0.0	2.3151405279017245	0.0
12	9.481674767177476E-5	0.0	0.0	2.653731133837632	0.0
13	9.481674767177476E-5	0.0	0.0	2.748073797771048	0.0
14	9.481674767177476E-5	0.0	0.0	2.7854315963537273	0.0
15	9.481674767177476E-5	0.0	0.0	2.8481054665647703	0.0
16	9.481674767177476E-5	0.0	0.0	2.955912108667578	0.0
17	9.481674767177476E-5	0.0	0.0	3.0762345614630604	0.0
18	9.481674767177476E-5	0.0	0.0	3.2707037109378705	0.0
19	9.481674767177476E-5	0.0	0.0	3.3633396734131944	0.0
20	2.844502430153243E-4	0.0	0.0	3.4475369453457305	0.0
21	2.844502430153243E-4	0.0	0.0	3.5471893471487657	0.0
22	3.7926699068709904E-4	0.0	0.0	3.666279182224515	0.0
23	3.7926699068709904E-4	0.0	0.0	3.7931439906093494	0.0
24	4.740837383588738E-4	0.0	0.0	3.89469272736582	0.0
25	4.740837383588738E-4	0.0	0.0	3.968080890063774	0.0
26	4.740837383588738E-4	0.0	0.0	4.051140361024248	0.0
27	4.740837383588738E-4	0.0	0.0	4.146526009182054	0.0
28	4.740837383588738E-4	0.0	0.0	4.256323802985969	0.0
29	4.740837383588738E-4	0.0	0.0	4.360242958434235	0.0
30	5.689004860306486E-4	0.0	0.0	4.477910542294906	0.0
31	5.689004860306486E-4	0.0	0.0	4.578321478079316	0.0
32	5.689004860306486E-4	0.0	0.0	4.672948592255747	0.0
33	5.689004860306486E-4	0.0	0.0	4.801046018360315	0.0
34	5.689004860306486E-4	0.0	0.0	4.922032188389499	0.0
35	5.689004860306486E-4	0.0	0.0	5.101615108479841	0.0
36	5.689004860306486E-4	0.0	0.0	5.2154900224336425	0.0
37	5.689004860306486E-4	0.0	0.0	5.327278967938665	0.0
38	7.585339813741981E-4	0.0	0.0	5.464668435315066	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATC	20	7.856187E-4	44.0	32
TCGTTAA	25	4.4425895E-5	44.0	35
GATCGTA	20	7.856187E-4	44.0	9
CGAGTTA	20	7.856187E-4	44.0	32
ACGATTG	55	1.8189894E-12	44.0	1
GTATGCG	25	4.4425895E-5	44.0	1
TACGACA	20	7.856187E-4	44.0	42
CGTCGCA	25	4.4425895E-5	44.0	36
TCGAGTA	20	7.856187E-4	44.0	21
GACGATT	20	7.856187E-4	44.0	44
CGCAACA	25	4.4425895E-5	44.0	37
CGTTTTT	13645	0.0	43.32283	1
GCGTAAG	70	0.0	40.857143	1
CGTTGAT	65	0.0	40.615383	25
TACGGGA	170	0.0	40.11765	4
TCACGAC	280	0.0	40.07143	25
CGGTCTA	280	0.0	40.07143	31
GATCGCT	55	7.8216544E-11	40.0	10
CGCATCG	55	7.8216544E-11	40.0	21
TAAGGGC	365	0.0	39.78082	4
>>END_MODULE