FastQCFastQC Report
Thu 26 May 2016
SRR1546136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546136_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1060785
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238322.246638102914351No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT31690.2987410266924966No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT30780.2901624740168837No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT28410.26782052913644144No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCC26740.25207747092954746No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT26330.24821240873504055No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATG17410.16412373855211No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT14000.13197773347096725No Hit
GGGCAGGGGCTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT13230.12471895813006406No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT10950.10322544153622082No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG302.5275313E-644.01
GCGAACG454.802132E-1044.01
ATCGTTC207.856209E-444.035
TCGGATT207.856209E-444.012
CCGTAGG302.5275313E-644.02
GTATGCG207.856209E-444.01
GTAAGCG254.442607E-544.01
GATTACG254.442607E-544.01
CGATAGT207.856209E-444.010
CCCGCTA351.445951E-744.038
CGTTTTT140050.043.371651
CGCATCG900.041.55555721
CGGTCTA3150.041.20635231
TAGGGTA8100.040.1975335
GCGTAAG1100.040.0000041
GTTTTTT155650.039.9717332
TCACGAC3250.039.9384625
AGGGTAC9450.039.8095256
CATATGC16700.039.7844333
TAACGGG2050.039.7073173