##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546136_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1060785
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.799771867060713	31.0	28.0	31.0	16.0	33.0
2	28.804823786158362	31.0	28.0	33.0	16.0	33.0
3	28.3196208468257	31.0	28.0	33.0	16.0	34.0
4	32.31744604231772	35.0	32.0	35.0	26.0	37.0
5	33.76077621761243	35.0	33.0	35.0	30.0	37.0
6	33.55012844261561	35.0	33.0	37.0	28.0	37.0
7	34.40000659888668	35.0	35.0	37.0	31.0	37.0
8	34.73492649311595	35.0	35.0	37.0	32.0	37.0
9	36.55455818096976	39.0	37.0	39.0	32.0	39.0
10	36.07942608539902	38.0	35.0	39.0	31.0	39.0
11	36.32447197122885	38.0	35.0	39.0	32.0	39.0
12	35.091776373157614	37.0	34.0	39.0	28.0	39.0
13	35.54045447475219	37.0	35.0	39.0	30.0	39.0
14	36.30965087176006	38.0	35.0	40.0	30.0	41.0
15	36.761434220883594	38.0	35.0	40.0	31.0	41.0
16	36.36093930438307	38.0	34.0	40.0	31.0	41.0
17	35.79618584350269	37.0	34.0	40.0	30.0	41.0
18	35.578911843587534	38.0	34.0	39.0	29.0	40.0
19	35.054218338306065	37.0	33.0	39.0	27.0	40.0
20	35.24269385407976	36.0	34.0	39.0	29.0	40.0
21	35.49113533845218	36.0	34.0	39.0	30.0	40.0
22	35.86780261787261	37.0	34.0	40.0	31.0	40.0
23	36.25475661891901	37.0	35.0	40.0	31.0	41.0
24	35.85618574923288	37.0	35.0	40.0	31.0	41.0
25	35.60725783264281	36.0	34.0	40.0	30.0	41.0
26	35.70017487049685	37.0	34.0	40.0	30.0	41.0
27	36.037262027649334	37.0	35.0	40.0	31.0	41.0
28	35.93678172296931	37.0	35.0	40.0	31.0	41.0
29	35.98774869554151	37.0	35.0	40.0	31.0	41.0
30	35.644871486682035	37.0	35.0	40.0	30.0	41.0
31	34.97028238521472	36.0	34.0	40.0	28.0	41.0
32	34.91208020475403	37.0	34.0	40.0	27.0	41.0
33	34.58190585274113	37.0	34.0	40.0	24.0	41.0
34	34.16500987476256	37.0	34.0	40.0	21.0	41.0
35	33.89524267405742	37.0	33.0	40.0	19.0	41.0
36	33.53600399703993	36.0	33.0	40.0	18.0	41.0
37	33.46018467455705	36.0	33.0	40.0	18.0	41.0
38	33.285934473055335	36.0	33.0	40.0	17.0	41.0
39	33.098790989691594	36.0	33.0	40.0	15.0	41.0
40	32.85514972402513	36.0	32.0	40.0	15.0	40.0
41	32.51216410488459	35.0	32.0	39.0	14.0	40.0
42	32.71584157015795	36.0	32.0	40.0	15.0	40.0
43	32.531973962678585	35.0	32.0	39.0	14.0	40.0
44	32.3664210938126	35.0	31.0	39.0	12.0	40.0
45	32.21969861941864	35.0	31.0	39.0	12.0	40.0
46	32.02836012952672	35.0	31.0	39.0	12.0	40.0
47	31.96383433023657	35.0	31.0	38.0	12.0	40.0
48	31.93648760116329	35.0	31.0	38.0	12.0	40.0
49	31.962936881648968	35.0	31.0	38.0	12.0	40.0
50	32.2197570667006	35.0	31.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	12.0
12	8.0
13	17.0
14	48.0
15	121.0
16	290.0
17	700.0
18	1408.0
19	2138.0
20	3312.0
21	4797.0
22	6802.0
23	9563.0
24	13874.0
25	20994.0
26	29801.0
27	34036.0
28	32577.0
29	31505.0
30	33781.0
31	40164.0
32	52704.0
33	77950.0
34	116736.0
35	99817.0
36	109467.0
37	149677.0
38	158466.0
39	30014.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.67011694169884	21.44157392874145	24.67644244592448	23.211866683635233
2	41.47409701306108	19.988310543606858	26.242263983747883	12.295328459584177
3	30.434630957262783	18.960958158344997	37.745348963267766	12.859061921124452
4	26.977379959181174	22.862974118223768	35.77982343264658	14.379822489948483
5	20.02187059583233	27.49133896124097	36.12673633205598	16.360054110870724
6	18.261193361520007	38.072088123418034	34.183364206695984	9.483354308365973
7	74.52000169685658	4.718298241396701	18.21198452089726	2.549715540849465
8	75.17404563601484	4.470651451519394	17.36110521924801	2.9941976932177585
9	71.71990554165076	4.9718840292802025	18.48357584241859	4.824634586650452
10	38.015526237644764	24.049642481747007	25.042303577067926	12.892527703540303
11	33.22690271826996	21.2730195091371	29.266816555663965	16.233261216928973
12	29.363160301097775	19.016388806402805	35.66773662900587	15.952714263493545
13	20.679402517946617	27.28413391969155	36.09232785154391	15.944135710817934
14	16.518050311797396	33.4457029464029	35.42772569370796	14.608521048091744
15	15.912932403833011	23.68943753918089	44.13816183298218	16.25946822400392
16	17.979703709988357	17.644291727352858	45.34566382443191	19.03034073822688
17	17.06877453960982	17.884019853221904	36.13126128291783	28.915944324250436
18	20.033088703177366	23.26560047511984	38.20679968136804	18.49451114033475
19	23.76862417926347	25.455488152641674	32.112067949678774	18.66381971841608
20	24.825294475317808	22.357687938649207	32.19172593880947	20.625291647223516
21	22.278407028757005	23.95678671926922	35.41245398454918	18.352352267424596
22	19.571543715267467	19.511965195586285	34.3522014357292	26.564289653417045
23	17.9717850459801	27.110017581319497	32.77987528104187	22.138322091658537
24	19.33860301569121	24.98819270634483	39.04759211338772	16.625612164576236
25	23.354402635783877	20.931479988876163	36.11740362090339	19.596713754436575
26	16.711869040380474	25.81729568197137	33.26630749869201	24.20452777895615
27	18.07538756675481	24.35837610825945	37.470835277648156	20.095401047337585
28	16.910684068873522	24.388542447338526	41.40857949537371	17.29219398841424
29	18.63629293400642	25.13242551506667	37.77315855710629	18.458122993820613
30	20.067214374260573	23.17265044283243	40.29666709088081	16.463468092026186
31	27.407061751438793	23.094029421607583	31.565114514251235	17.933794312702386
32	24.826708522462138	25.49234764820392	32.04419368675085	17.63675014258309
33	23.227892551270994	23.186225295418016	30.272392614903115	23.31348953840788
34	19.082754752376776	25.80042138604901	31.787779804578687	23.329044056995528
35	19.856521349755134	23.66502165848876	33.179107924791545	23.29934906696456
36	28.167913384898917	24.283620149229108	28.774256800388393	18.77420966548358
37	19.61085422587989	27.67563643905221	31.058791366770834	21.654717968297064
38	21.355976941604567	30.84140518578223	26.894799605952198	20.90781826666101
39	19.520449478452278	25.302016902576863	29.39087562512667	25.78665799384418
40	21.12303624202831	23.40125473116607	33.987942891349334	21.48776613545629
41	18.708032259128853	22.554523301140193	34.26519040144799	24.47225403828297
42	22.02321865410993	27.00698067940252	27.608799144030126	23.361001522457425
43	20.695428385582375	23.872886588705533	29.674627752089254	25.757057273622834
44	20.365766861333825	25.085196340445993	32.806647906974554	21.742388891245636
45	21.5692152509698	28.189312631683137	29.30216773427226	20.9393043830748
46	24.483095066389513	25.228203641642743	29.930476015403684	20.358225276564053
47	22.32808721842786	26.620380190142207	29.79482175935746	21.256710832072475
48	20.462204876577253	28.603911254401222	30.226106138378654	20.707777730642874
49	20.54044881856361	26.567400557134572	31.848018212927222	21.044132411374594
50	20.018476882685935	25.49875799525823	33.47322973081256	21.00953539124328
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	760.0
1	1245.0
2	1730.0
3	19023.5
4	36317.0
5	24907.5
6	13498.0
7	13567.5
8	13637.0
9	13929.5
10	14222.0
11	13955.0
12	13688.0
13	12928.5
14	12169.0
15	11152.5
16	10136.0
17	9449.5
18	8763.0
19	8008.0
20	7253.0
21	6836.0
22	6419.0
23	6278.5
24	6138.0
25	6311.0
26	6484.0
27	7657.0
28	8830.0
29	10275.0
30	11720.0
31	12404.5
32	13089.0
33	16058.0
34	19027.0
35	20323.0
36	21619.0
37	23992.0
38	26365.0
39	32043.5
40	37722.0
41	46995.5
42	56269.0
43	68549.5
44	80830.0
45	83845.5
46	86861.0
47	89024.5
48	91188.0
49	93516.0
50	95844.0
51	85977.5
52	76111.0
53	65906.0
54	55701.0
55	51084.0
56	46467.0
57	44787.0
58	43107.0
59	39822.5
60	36538.0
61	32729.0
62	28920.0
63	26074.5
64	23229.0
65	19279.0
66	15329.0
67	13199.0
68	11069.0
69	9961.5
70	8854.0
71	7918.0
72	6982.0
73	5463.5
74	3945.0
75	2995.5
76	2046.0
77	1456.5
78	867.0
79	683.5
80	500.0
81	405.0
82	310.0
83	207.5
84	105.0
85	81.0
86	57.0
87	37.5
88	18.0
89	22.0
90	26.0
91	18.5
92	11.0
93	9.0
94	7.0
95	6.5
96	6.0
97	3.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1060785.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.997213688376455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.07147872391103	21.577866591124963
2	7.40589513421768	4.146888572527995
3	3.212095193317194	2.6978914654412507
4	1.938436445231656	2.170832775139501
5	1.426820129394915	1.9973494028773187
6	1.1079203942348326	1.8611210416261736
7	0.8372004119890287	1.6407495184117549
8	0.6844474010131456	1.5330096115695144
9	0.5317670786780634	1.3399196880774091
>10	4.608599771285236	27.71979031003879
>50	0.9148697527294941	18.137310839639536
>100	0.2492629204359433	9.498973943004628
>500	0.007471095707887976	1.4177913624340968
>1k	0.003395952594494535	1.994624798317122
>5k	0.0	0.0
>10k+	3.3959525944945345E-4	2.265880079769943
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23832	2.246638102914351	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3169	0.2987410266924966	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3078	0.2901624740168837	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2841	0.26782052913644144	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCC	2674	0.25207747092954746	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT	2633	0.24821240873504055	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATG	1741	0.16412373855211	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1400	0.13197773347096725	No Hit
GGGCAGGGGCTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1323	0.12471895813006406	No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1095	0.10322544153622082	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04345838223579707	0.0
2	0.0	0.0	0.0	0.1903307456270592	0.0
3	0.0	0.0	0.0	0.24915510683126174	0.0
4	9.426980962211947E-5	0.0	0.0	0.40460602289813674	0.0
5	9.426980962211947E-5	0.0	0.0	0.8674707881427434	0.0
6	9.426980962211947E-5	0.0	0.0	1.081840335223443	0.0
7	9.426980962211947E-5	0.0	0.0	1.241062043675203	0.0
8	9.426980962211947E-5	0.0	0.0	1.5279250743553123	0.0
9	9.426980962211947E-5	0.0	0.0	1.6156902671135056	0.0
10	9.426980962211947E-5	0.0	0.0	2.041224187747753	0.0
11	9.426980962211947E-5	0.0	0.0	2.2395678671926924	0.0
12	9.426980962211947E-5	0.0	0.0	2.577902213926479	0.0
13	2.828094288663584E-4	0.0	0.0	2.666421565161649	0.0
14	3.770792384884779E-4	0.0	0.0	2.702715441866165	0.0
15	3.770792384884779E-4	0.0	0.0	2.7714381330806903	0.0
16	3.770792384884779E-4	0.0	0.0	2.88352493672139	0.0
17	3.770792384884779E-4	0.0	0.0	3.011071989140118	0.0
18	3.770792384884779E-4	0.0	0.0	3.2034766705788638	0.0
19	3.770792384884779E-4	0.0	0.0	3.3088703177363934	0.0
20	3.770792384884779E-4	0.0	0.0	3.3857002125784206	0.0
21	4.7134904811059735E-4	0.0	0.0	3.48128979953525	0.0
22	4.7134904811059735E-4	0.0	0.0	3.592716714508595	0.0
23	4.7134904811059735E-4	0.0	0.0	3.7241288291218297	0.0
24	5.656188577327168E-4	0.0	0.0	3.827637080086917	0.0
25	5.656188577327168E-4	0.0	0.0	3.8997534844478383	0.0
26	5.656188577327168E-4	0.0	0.0	3.9844077734885013	0.0
27	6.598886673548363E-4	0.0	0.0	4.07952601139722	0.0
28	7.541584769769558E-4	0.0	0.0	4.185862356650971	0.0
29	9.426980962211947E-4	0.0	0.0	4.290219035902657	0.0
30	9.426980962211947E-4	0.0	0.0	4.3958954924890525	0.0
31	9.426980962211947E-4	0.0	0.0	4.498272505738675	0.0
32	9.426980962211947E-4	0.0	0.0	4.592636585170416	0.0
33	0.0010369679058433142	0.0	0.0	4.719335209302545	0.0
34	0.0010369679058433142	0.0	0.0	4.83698393171095	0.0
35	0.0010369679058433142	0.0	0.0	5.005726890934544	0.0
36	0.0010369679058433142	0.0	0.0	5.1198876303869305	0.0
37	0.0010369679058433142	0.0	0.0	5.234236909458561	0.0
38	0.0010369679058433142	0.0	0.0	5.36894846740857	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	30	2.5275313E-6	44.0	1
GCGAACG	45	4.802132E-10	44.0	1
ATCGTTC	20	7.856209E-4	44.0	35
TCGGATT	20	7.856209E-4	44.0	12
CCGTAGG	30	2.5275313E-6	44.0	2
GTATGCG	20	7.856209E-4	44.0	1
GTAAGCG	25	4.442607E-5	44.0	1
GATTACG	25	4.442607E-5	44.0	1
CGATAGT	20	7.856209E-4	44.0	10
CCCGCTA	35	1.445951E-7	44.0	38
CGTTTTT	14005	0.0	43.37165	1
CGCATCG	90	0.0	41.555557	21
CGGTCTA	315	0.0	41.206352	31
TAGGGTA	810	0.0	40.197533	5
GCGTAAG	110	0.0	40.000004	1
GTTTTTT	15565	0.0	39.971733	2
TCACGAC	325	0.0	39.93846	25
AGGGTAC	945	0.0	39.809525	6
CATATGC	1670	0.0	39.78443	33
TAACGGG	205	0.0	39.707317	3
>>END_MODULE