FastQCFastQC Report
Thu 26 May 2016
SRR1546135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546135_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1045579
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238302.279119989976845No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT31600.30222489166289684No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT29990.28682672471424925No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT28100.26875061568757597No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCC26810.2564129539709577No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT26750.25583910923995223No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATG17190.16440651543307583No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT13900.13294069601627423No Hit
GGGCAGGGGCTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT13610.13016711314974763No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT10460.1000402647719589No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAT254.442563E-544.032
CCGTCGA254.442563E-544.035
GTAGACG207.856156E-444.01
AATTCGC800.044.013
ATGCGTA207.856156E-444.043
TATAGCG207.856156E-444.01
CCAACGA254.442563E-544.031
ATACGAG351.4459147E-744.01
GCGTAAG408.310963E-944.01
CTTACGT207.856156E-444.021
CGTTTTT134450.043.329121
CGGTCTA2850.041.6842131
AGGGTAC8150.041.3006136
TCACGAC2950.041.0169525
ATCGTAG700.040.8571431
ATAGCGG1350.040.740742
CCGCAAT650.040.61538333
CATATGC15650.040.48562633
CTCACGA2950.040.27118724
GTTGATC16150.040.18575716