##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546135_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1045579
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.684829171205617	31.0	28.0	31.0	16.0	33.0
2	28.709249133733557	31.0	28.0	33.0	16.0	33.0
3	28.22159301210143	31.0	27.0	33.0	16.0	34.0
4	32.21714762825191	35.0	32.0	35.0	26.0	37.0
5	33.71274480455327	35.0	33.0	35.0	30.0	37.0
6	33.49237790736042	35.0	33.0	37.0	28.0	37.0
7	34.31249862516366	35.0	35.0	37.0	31.0	37.0
8	34.69789179009907	35.0	35.0	37.0	32.0	37.0
9	36.53761504391347	39.0	37.0	39.0	32.0	39.0
10	36.16331716685205	38.0	35.0	39.0	31.0	39.0
11	36.344973454899154	38.0	35.0	39.0	32.0	39.0
12	35.27134248105595	37.0	34.0	39.0	29.0	39.0
13	35.612188079523406	37.0	35.0	39.0	30.0	39.0
14	36.37739855142462	38.0	35.0	40.0	30.0	41.0
15	36.73164724999259	38.0	35.0	40.0	31.0	41.0
16	36.32078972511881	38.0	34.0	40.0	31.0	41.0
17	35.74717453200571	37.0	34.0	40.0	29.0	41.0
18	35.514179225099205	37.0	34.0	39.0	29.0	40.0
19	34.95244835636523	37.0	33.0	39.0	27.0	40.0
20	35.330015235577605	36.0	34.0	39.0	30.0	40.0
21	35.57435449640821	36.0	34.0	39.0	30.0	40.0
22	35.885644222005226	37.0	34.0	40.0	31.0	41.0
23	36.23265195647579	37.0	35.0	40.0	31.0	41.0
24	35.8521001282543	37.0	35.0	40.0	31.0	41.0
25	35.61276957551749	36.0	34.0	40.0	30.0	41.0
26	35.698321217239446	37.0	34.0	40.0	30.0	41.0
27	36.02763349302157	37.0	35.0	40.0	31.0	41.0
28	35.936996630575024	37.0	35.0	40.0	31.0	41.0
29	35.96990949512184	37.0	35.0	40.0	31.0	41.0
30	35.6154226509905	37.0	35.0	40.0	30.0	41.0
31	34.92616435486941	36.0	34.0	40.0	27.0	41.0
32	34.883792616339846	37.0	34.0	40.0	26.0	41.0
33	34.563631251201485	37.0	34.0	40.0	24.0	41.0
34	34.15037601175999	37.0	34.0	40.0	21.0	41.0
35	33.842946348386874	37.0	33.0	40.0	18.0	41.0
36	33.530273656988136	36.0	33.0	40.0	18.0	41.0
37	33.471235554654406	37.0	33.0	40.0	18.0	41.0
38	33.273292596733484	36.0	33.0	40.0	16.0	41.0
39	33.06187193889701	36.0	33.0	40.0	15.0	41.0
40	32.879078481874636	36.0	32.0	40.0	15.0	41.0
41	32.5229074034578	35.0	32.0	39.0	14.0	40.0
42	32.75161800303946	36.0	32.0	40.0	14.0	41.0
43	32.55770056590654	35.0	32.0	39.0	13.0	40.0
44	32.40374950147239	35.0	32.0	39.0	12.0	40.0
45	32.286823855490596	35.0	31.0	39.0	12.0	40.0
46	32.101999944528345	35.0	31.0	39.0	12.0	40.0
47	32.05537410372626	35.0	31.0	39.0	12.0	40.0
48	32.01652481543719	35.0	31.0	39.0	12.0	40.0
49	32.047836653184504	35.0	31.0	39.0	12.0	40.0
50	32.23269021279119	35.0	31.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	6.0
12	8.0
13	20.0
14	35.0
15	104.0
16	266.0
17	690.0
18	1310.0
19	2154.0
20	3279.0
21	4883.0
22	6741.0
23	9505.0
24	14044.0
25	21203.0
26	29313.0
27	33610.0
28	32174.0
29	31123.0
30	33470.0
31	40296.0
32	51231.0
33	76027.0
34	114819.0
35	97594.0
36	106424.0
37	144610.0
38	157146.0
39	33489.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.592714658576732	21.47699982497736	24.631424311314593	23.29886120513132
2	41.47778407944306	19.989689922999602	26.205767330828184	12.326758666729152
3	30.494395927997786	19.030221532758404	37.634363352745225	12.841019186498581
4	26.967737492814987	22.920888808975697	35.69266406459962	14.418709633609703
5	20.039136210654576	27.576969315565826	36.05935084771213	16.32454362606747
6	18.167254698114633	38.02562981850247	34.20573672577586	9.60137875760703
7	74.4187670180828	4.766067413366183	18.259069855075513	2.5560957134755
8	75.03804112362624	4.547719493218589	17.398494040144264	3.015745343010906
9	71.61381397292791	5.019993706836116	18.5291594418021	4.837032878433863
10	38.05279180243674	24.230115562764745	25.03990611900201	12.67718651579651
11	33.158661373267826	21.320244572624354	29.242649288097788	16.27844476601003
12	29.26531615497251	19.370798380610168	35.72575577742093	15.638129686996391
13	20.630483205955745	27.325434041808418	36.15374830596254	15.890334446273307
14	16.520415960917347	33.44118426249953	35.387187386127685	14.65121239045543
15	15.973446291480606	23.668704134264367	44.11842624995337	16.239423324301654
16	17.97893798555633	17.722333750008367	45.31489251409984	18.98383575033546
17	17.02989444126173	17.94708960298552	36.18712694114936	28.835889014603396
18	20.054725659180225	23.331761636375635	38.22733624145091	18.38617646299323
19	23.703039177336194	25.53446463634025	32.15146822956467	18.611027956758885
20	24.819645383084396	22.525987993255413	32.20502707112519	20.449339552535008
21	22.28592961411811	24.127397355914763	35.20680885901496	18.37986417095217
22	19.578243250868656	19.658103309266924	34.33944254810014	26.424210891764275
23	18.12498146959723	27.118563016280934	32.68763048990081	22.06882502422103
24	19.345549212445928	24.973627052570873	39.02469349518305	16.65613023980015
25	23.279159202700132	21.03609578998813	36.1295511864718	19.555193820839936
26	16.64991358854759	25.94275516245066	33.24770294736218	24.15962830163957
27	17.953688817392084	24.508525898090912	37.48870243185833	20.04908285265867
28	16.965719472177618	24.363343181146526	41.40767938147189	17.26325796520397
29	18.652727340545287	25.116227468225738	37.87757787790306	18.35346731332592
30	20.020486256896895	23.239468275472248	40.29021240862718	16.449833059003673
31	27.468608302194287	23.074105352154163	31.591395772103304	17.865890573548246
32	24.816393596275365	25.43662410970381	32.046167721425164	17.700814572595664
33	23.243580829377787	23.14975721585839	30.27863030913972	23.3280316456241
34	19.156563014368118	25.77863556938309	31.801709866016818	23.263091550231977
35	19.837716710071646	23.700361235258168	33.298201283690666	23.163720770979523
36	28.17128117531052	24.25459960462098	28.810448564862146	18.76367065520635
37	19.531953109234212	27.736211228419847	31.10917491648168	21.622660745864252
38	21.37380341418487	30.836407387677067	26.897154590901305	20.892634607236758
39	19.550029218260885	25.323959260849733	29.45726721749385	25.668744303395535
40	21.041260392567178	23.487560480843626	34.01426386719702	21.456915259392165
41	18.765296548610866	22.603552672729656	34.23385511759513	24.397295661064348
42	22.049983788886347	27.05381420246581	27.615703834908693	23.280498173739144
43	20.658984160929016	24.010811234732145	29.669015923234877	25.661188681103965
44	20.305974010572132	25.223345151346766	32.76596029568306	21.70472054239804
45	21.59722029612301	28.241577154858692	29.26761153389653	20.893591015121764
46	24.44157734614027	25.29459753877995	30.074341584901767	20.189483530178016
47	22.370858634306927	26.692100740355347	29.725061425296417	21.211979200041316
48	20.47659717725777	28.634660795597462	30.199439736260963	20.689302290883806
49	20.519252968929177	26.59885097156695	31.916478812217917	20.965417247285952
50	19.97821302837949	25.648085893079337	33.47762340291838	20.89607767562279
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	808.0
1	1267.5
2	1727.0
3	18981.0
4	36235.0
5	24814.0
6	13393.0
7	13492.0
8	13591.0
9	13858.5
10	14126.0
11	13733.0
12	13340.0
13	12593.0
14	11846.0
15	11014.5
16	10183.0
17	9305.0
18	8427.0
19	7807.5
20	7188.0
21	6728.5
22	6269.0
23	6155.5
24	6042.0
25	6273.0
26	6504.0
27	7542.5
28	8581.0
29	9982.0
30	11383.0
31	12209.0
32	13035.0
33	15945.0
34	18855.0
35	20110.0
36	21365.0
37	23926.0
38	26487.0
39	32123.5
40	37760.0
41	46680.0
42	55600.0
43	67707.5
44	79815.0
45	82942.5
46	86070.0
47	87973.0
48	89876.0
49	91842.5
50	93809.0
51	84241.0
52	74673.0
53	64935.0
54	55197.0
55	50301.5
56	45406.0
57	43653.5
58	41901.0
59	38910.5
60	35920.0
61	32254.0
62	28588.0
63	25710.0
64	22832.0
65	18886.0
66	14940.0
67	12812.5
68	10685.0
69	9617.5
70	8550.0
71	7658.5
72	6767.0
73	5327.5
74	3888.0
75	2958.0
76	2028.0
77	1420.0
78	812.0
79	674.5
80	537.0
81	420.5
82	304.0
83	208.5
84	113.0
85	82.0
86	51.0
87	41.0
88	31.0
89	25.5
90	20.0
91	14.5
92	9.0
93	8.5
94	8.0
95	6.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1045579.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.797406984291197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.95836980531797	21.3924312632601
2	7.254173883073775	4.032944475252355
3	3.2151341465011023	2.6811717713814853
4	2.0319689416790125	2.2593387060516386
5	1.4415109920615925	2.0035133859332714
6	1.0983641905372035	1.8319005852800525
7	0.8895779004522251	1.7309571260171241
8	0.70174739069893	1.5605406255538044
9	0.5742610767879078	1.4366671980039694
>10	4.6423152400977274	27.730283172523524
>50	0.9366780824645948	18.395561537723008
>100	0.24481750159928017	9.25461921584612
>500	0.0072718069781957965	1.3895500904868174
>1k	0.0034627652277122835	2.0067428849036553
>5k	0.0	0.0
>10k+	3.462765227712284E-4	2.293777961783102
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23830	2.279119989976845	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	3160	0.30222489166289684	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2999	0.28682672471424925	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2810	0.26875061568757597	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCC	2681	0.2564129539709577	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTT	2675	0.25583910923995223	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATG	1719	0.16440651543307583	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1390	0.13294069601627423	No Hit
GGGCAGGGGCTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1361	0.13016711314974763	No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1046	0.1000402647719589	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.041029898266893274	0.0
2	0.0	0.0	0.0	0.189464402020316	0.0
3	0.0	0.0	0.0	0.24522298171635046	0.0
4	0.0	0.0	0.0	0.4023607972233566	0.0
5	0.0	0.0	0.0	0.8587586399497312	0.0
6	0.0	0.0	0.0	1.0571176353006324	0.0
7	0.0	0.0	0.0	1.2140641692306369	0.0
8	0.0	0.0	0.0	1.500890893944886	0.0
9	9.564078850091671E-5	0.0	0.0	1.587063244384212	0.0
10	9.564078850091671E-5	0.0	0.0	2.0114214229627794	0.0
11	9.564078850091671E-5	0.0	0.0	2.203850689426624	0.0
12	9.564078850091671E-5	0.0	0.0	2.543758051758882	0.0
13	9.564078850091671E-5	0.0	0.0	2.631843217968226	0.0
14	9.564078850091671E-5	0.0	0.0	2.666369542617057	0.0
15	9.564078850091671E-5	0.0	0.0	2.73236168668269	0.0
16	9.564078850091671E-5	0.0	0.0	2.8316368251466413	0.0
17	9.564078850091671E-5	0.0	0.0	2.9518572962922938	0.0
18	9.564078850091671E-5	0.0	0.0	3.1285058326534867	0.0
19	1.9128157700183343E-4	0.0	0.0	3.222138164595884	0.0
20	2.8692236550275014E-4	0.0	0.0	3.3038153979756673	0.0
21	2.8692236550275014E-4	0.0	0.0	3.3972564483410626	0.0
22	2.8692236550275014E-4	0.0	0.0	3.5087736077331315	0.0
23	2.8692236550275014E-4	0.0	0.0	3.6360714972278516	0.0
24	3.8256315400366685E-4	0.0	0.0	3.7396504711743446	0.0
25	4.7820394250458356E-4	0.0	0.0	3.8117636257040357	0.0
26	4.7820394250458356E-4	0.0	0.0	3.8890413828127763	0.0
27	5.738447310055003E-4	0.0	0.0	3.9820998700241685	0.0
28	5.738447310055003E-4	0.0	0.0	4.088261145260186	0.0
29	5.738447310055003E-4	0.0	0.0	4.187153720570134	0.0
30	5.738447310055003E-4	0.0	0.0	4.299053443116207	0.0
31	5.738447310055003E-4	0.0	0.0	4.396128843444637	0.0
32	6.69485519506417E-4	0.0	0.0	4.488900408290526	0.0
33	7.651263080073337E-4	0.0	0.0	4.61658086093925	0.0
34	7.651263080073337E-4	0.0	0.0	4.735558001834391	0.0
35	7.651263080073337E-4	0.0	0.0	4.895469400207923	0.0
36	7.651263080073337E-4	0.0	0.0	5.0074647635424965	0.0
37	8.607670965082504E-4	0.0	0.0	5.120033971608075	0.0
38	8.607670965082504E-4	0.0	0.0	5.252018259739341	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGAT	25	4.442563E-5	44.0	32
CCGTCGA	25	4.442563E-5	44.0	35
GTAGACG	20	7.856156E-4	44.0	1
AATTCGC	80	0.0	44.0	13
ATGCGTA	20	7.856156E-4	44.0	43
TATAGCG	20	7.856156E-4	44.0	1
CCAACGA	25	4.442563E-5	44.0	31
ATACGAG	35	1.4459147E-7	44.0	1
GCGTAAG	40	8.310963E-9	44.0	1
CTTACGT	20	7.856156E-4	44.0	21
CGTTTTT	13445	0.0	43.32912	1
CGGTCTA	285	0.0	41.68421	31
AGGGTAC	815	0.0	41.300613	6
TCACGAC	295	0.0	41.01695	25
ATCGTAG	70	0.0	40.857143	1
ATAGCGG	135	0.0	40.74074	2
CCGCAAT	65	0.0	40.615383	33
CATATGC	1565	0.0	40.485626	33
CTCACGA	295	0.0	40.271187	24
GTTGATC	1615	0.0	40.185757	16
>>END_MODULE