FastQCFastQC Report
Thu 26 May 2016
SRR1546134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546134_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences896685
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120351.3421658664971534No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT20490.22850833904882986No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT19280.21501419115966028No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT15960.1779889258769802No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCGGGAATATTCGTATG10550.11765558696755271No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT207.855554E-444.00000419
GTAATAC207.855554E-444.00000419
ACCGTCT207.855554E-444.00000414
TCGTTAG254.4420543E-544.01
ACGTTAG254.4420543E-544.01
TTATACG302.527091E-644.01
CCCAACG254.4420543E-544.015
TGCGATA254.4420543E-544.034
ATAACGC750.044.011
CGTTTTT118750.043.4071581
CGTAAGG1650.041.3333322
TAACGGG2400.041.253
CGGTCTA2200.041.031
TAGGGTA6600.040.6666685
ATTATGA7600.040.52631426
ACGGGAT2100.039.809525
TAGTAAG501.3478711E-939.6000021
GTTTTTT132350.039.4786532
CTAGGCG950.039.368424
AAGGGTA3700.039.243245
Produced by FastQC (version 0.11.5)