##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546134_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	896685
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.429283416138333	28.0	16.0	30.0	16.0	33.0
2	25.950966058314794	30.0	25.0	31.0	10.0	33.0
3	26.3598443154508	30.0	25.0	31.0	16.0	33.0
4	28.318883442903584	32.0	19.0	35.0	10.0	37.0
5	31.352539632089307	33.0	30.0	35.0	19.0	37.0
6	29.700460027768948	33.0	28.0	35.0	17.0	36.0
7	29.72205289482929	32.0	28.0	35.0	17.0	35.0
8	32.281084215750234	33.0	32.0	35.0	28.0	35.0
9	26.860370141130943	30.0	23.0	34.0	10.0	35.0
10	30.94130491755745	34.0	27.0	37.0	17.0	37.0
11	33.34648064816518	35.0	32.0	37.0	26.0	39.0
12	32.888519379715284	35.0	31.0	38.0	23.0	39.0
13	33.348803648996025	35.0	32.0	38.0	24.0	39.0
14	33.134660443745574	36.0	31.0	39.0	22.0	40.0
15	31.79772383836018	34.0	27.0	38.0	17.0	40.0
16	30.44675443438889	33.0	26.0	38.0	17.0	40.0
17	30.27120672253913	32.0	25.0	37.0	17.0	40.0
18	31.420302558869615	34.0	27.0	38.0	17.0	39.0
19	31.15772316922888	34.0	27.0	37.0	18.0	39.0
20	31.34466172624723	34.0	29.0	37.0	18.0	39.0
21	32.62601247929875	34.0	31.0	37.0	23.0	39.0
22	33.06221359786324	35.0	31.0	37.0	25.0	39.0
23	34.31731767566091	35.0	33.0	38.0	28.0	40.0
24	33.9937703875943	35.0	33.0	38.0	26.0	40.0
25	32.96654789586086	35.0	31.0	38.0	23.0	40.0
26	26.545688842793176	33.0	20.0	36.0	0.0	39.0
27	29.850432426102813	34.0	24.0	37.0	15.0	39.0
28	31.26080173081963	34.0	27.0	37.0	19.0	39.0
29	32.419510753497605	35.0	30.0	37.0	23.0	39.0
30	32.31526232735018	35.0	30.0	38.0	22.0	39.0
31	31.167860508428266	34.0	29.0	37.0	18.0	39.0
32	31.465575982647195	34.0	30.0	37.0	18.0	39.0
33	31.01673608903907	34.0	29.0	37.0	17.0	39.0
34	30.943319002771318	34.0	29.0	37.0	15.0	39.0
35	29.99322839124107	34.0	27.0	37.0	10.0	39.0
36	29.89943402644184	34.0	27.0	37.0	10.0	39.0
37	30.124232032430562	34.0	27.0	37.0	10.0	39.0
38	29.813297869374416	33.0	27.0	37.0	10.0	39.0
39	29.900099812085628	34.0	27.0	37.0	10.0	39.0
40	29.633124229801993	33.0	26.0	37.0	10.0	39.0
41	28.91793216123834	33.0	24.0	36.0	9.0	38.0
42	29.147495497303957	33.0	25.0	36.0	9.0	38.0
43	28.797463992371902	33.0	24.0	36.0	9.0	38.0
44	28.549204012557365	33.0	24.0	36.0	8.0	38.0
45	27.822406976809024	31.0	23.0	35.0	8.0	38.0
46	27.374206103592677	31.0	23.0	35.0	8.0	38.0
47	27.343390376776682	31.0	23.0	34.0	8.0	37.0
48	27.343186291730095	31.0	23.0	34.0	8.0	37.0
49	27.19545771368987	31.0	23.0	34.0	8.0	37.0
50	29.212037672092205	33.0	25.0	36.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	13.0
12	47.0
13	150.0
14	360.0
15	886.0
16	1647.0
17	2906.0
18	4515.0
19	6712.0
20	10082.0
21	14146.0
22	18778.0
23	24712.0
24	31958.0
25	38999.0
26	45853.0
27	49927.0
28	54430.0
29	62418.0
30	73378.0
31	86100.0
32	94174.0
33	94929.0
34	83600.0
35	61939.0
36	28718.0
37	5150.0
38	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.895654549814036	22.299804279094666	24.3311753848899	24.4733657862014
2	41.716879394659216	17.39284141030574	27.538767794710516	13.351511400324528
3	32.02640838198476	14.930661269007512	39.27031231703441	13.772618031973325
4	28.47867422785036	22.62611730986913	34.65453308575475	14.240675376525758
5	19.315701723570708	27.639249011637308	36.81069717905396	16.234352085738024
6	18.01435286639121	36.81627327322304	35.388235556522076	9.781138303863676
7	76.81582718568951	3.1197131657159427	18.38326725661743	1.681192391977116
8	78.1782900349621	3.0412017598153196	16.840473521916838	1.9400346833057316
9	65.73847003128189	3.0191204269057694	16.537245521002358	14.705164020809983
10	38.80760802288429	24.21430045110602	25.09733072372126	11.880760802288428
11	34.10919107601889	20.707383306289277	29.50132989845933	15.682095719232505
12	29.575826516558212	19.008793500504638	36.4147944930494	15.000585489887753
13	20.063678995410875	27.810323580744633	36.83445134021424	15.291546083630262
14	15.884284893803285	33.718306874766505	36.04108466183777	14.356323569592444
15	14.249597127196283	22.036612634314167	43.83546061325884	19.878329625230712
16	16.088258418508172	15.378087065134356	45.573417643877164	22.9602368724803
17	16.169446349609952	16.8818481406514	36.88452466585256	30.064180843886092
18	19.39488226077162	22.921315735180137	39.22938378583338	18.45441821821487
19	23.963933822914402	25.138147733038917	32.82122484484518	18.076693599201505
20	25.0547293642695	21.74007594640258	32.7684749940057	20.436719695322214
21	22.198207843334057	23.659702125049485	35.840233749867565	18.301856281748886
22	19.13492475060919	19.05161790372316	34.675276156063724	27.138181189603934
23	17.40377055487713	27.38509063941072	33.13315155266342	22.07798725304873
24	18.60675711091409	24.48351427758912	40.794816462860425	16.114912148636364
25	23.12372795351768	19.624059731120738	37.56146249797867	19.690749817382915
26	13.229171894254952	38.05160117544065	27.702258875747894	21.016968054556507
27	17.070877732983156	24.993726894059787	38.36341636137551	19.571979011581547
28	16.199891823773118	24.04690610415029	43.00752215103409	16.745679921042505
29	17.92178970318451	24.94967575012407	38.96674975046979	18.16178479622164
30	19.380495937815397	22.781801859069795	41.52595393030998	16.31174827280483
31	28.04864584553104	22.670503019454994	31.956484161104516	17.324366973909456
32	24.755850716806904	25.181529745674347	32.41283170790189	17.649787829616866
33	23.1478166803281	22.557419829706085	30.63818397765101	23.656579512314803
34	18.56326357639528	25.23249524637972	31.777826103927243	24.426415073297758
35	19.340348059798036	23.047446985284687	33.36310967619621	24.249095278721068
36	28.496740772958173	23.465765569848944	28.535104300841436	19.50238935635145
37	18.901732492458333	27.900767828167083	30.621567217027163	22.575932462347424
38	20.958530587664566	31.409915410651458	26.58012568516257	21.05142831652141
39	18.92693643810257	25.037443472345362	29.257877627037367	26.77774246251471
40	20.88436853521582	22.7521370380903	34.21491382146461	22.148580605229263
41	18.106135376414237	21.713756781924534	34.57758298622147	25.602524855439757
42	21.394804195453254	26.65763339411276	26.684733211774482	25.26282919865951
43	20.001115218833814	23.207257844170474	29.561105627951846	27.230521309043866
44	19.62194081533649	24.512175401618183	33.1774257403659	22.688458042679425
45	21.10819295516263	28.816920100146653	28.807217696292454	21.267669248398267
46	23.97798558022048	24.843283873378052	29.56422824068653	21.61450230571494
47	22.02178022382442	26.054746092552012	29.03249190072322	22.89098178290035
48	19.583911853103373	28.265667430591567	30.607403937837702	21.543016778467354
49	19.88602463518404	26.597523098970093	31.407350407333677	22.109101858512187
50	19.535622877599156	25.697429978197473	33.53217685140267	21.234770292800707
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	445.0
1	740.5
2	1036.0
3	10905.5
4	20775.0
5	16210.0
6	11645.0
7	11488.0
8	11331.0
9	11938.5
10	12546.0
11	12696.0
12	12846.0
13	12634.5
14	12423.0
15	11906.5
16	11390.0
17	10822.0
18	10254.0
19	9495.5
20	8737.0
21	8243.0
22	7749.0
23	7361.0
24	6973.0
25	6883.5
26	6794.0
27	7624.5
28	8455.0
29	9470.0
30	10485.0
31	11354.0
32	12223.0
33	14263.0
34	16303.0
35	16940.0
36	17577.0
37	19269.0
38	20961.0
39	25348.0
40	29735.0
41	37296.5
42	44858.0
43	53090.5
44	61323.0
45	66398.0
46	71473.0
47	74260.0
48	77047.0
49	78188.5
50	79330.0
51	71527.0
52	63724.0
53	55665.5
54	47607.0
55	43767.0
56	39927.0
57	38443.5
58	36960.0
59	34274.5
60	31589.0
61	28583.5
62	25578.0
63	22962.0
64	20346.0
65	17187.5
66	14029.0
67	12010.0
68	9991.0
69	8957.5
70	7924.0
71	7198.0
72	6472.0
73	5130.0
74	3788.0
75	2884.5
76	1981.0
77	1460.0
78	939.0
79	749.5
80	560.0
81	433.0
82	306.0
83	218.5
84	131.0
85	92.5
86	54.0
87	40.0
88	26.0
89	22.5
90	19.0
91	13.5
92	8.0
93	9.0
94	10.0
95	6.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	896685.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.914340376271156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.73684673549666	36.333340492019786
2	6.947572613891638	6.101961371105946
3	2.7160401060253143	3.5781932907479783
4	1.51134816890182	2.6547943166483465
5	0.9748124706710151	2.14041233200404
6	0.6832549049249351	1.8002813075178234
7	0.525903515730207	1.6166294196397788
8	0.40798488961470025	1.4333109848732288
9	0.31551159608490187	1.2469935260819667
>10	2.762478160751784	25.303151157998037
>50	0.3555516593089171	10.564409101193178
>100	0.059072790501378945	4.309580625117585
>500	0.002229161905702578	0.6332400049666855
>1k	0.001114580952851289	0.8110366672307616
>5k	0.0	0.0
>10k+	2.7864523821282224E-4	1.472665402854891
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12035	1.3421658664971534	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2049	0.22850833904882986	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1928	0.21501419115966028	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1596	0.1779889258769802	No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCGGGAATATTCGTATG	1055	0.11765558696755271	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028884167795825737	0.0
2	0.0	0.0	0.0	0.13460691324155083	0.0
3	0.0	0.0	0.0	0.19349046766701797	0.0
4	0.0	0.0	0.0	0.2507011938417616	0.0
5	0.0	0.0	0.0	0.499394993782655	0.0
6	0.0	0.0	0.0	0.6105823115140768	0.0
7	0.0	0.0	0.0	0.6699119534730702	0.0
8	0.0	0.0	0.0	0.8124369204347123	0.0
9	0.0	0.0	0.0	0.8738854781779555	0.0
10	0.0	0.0	0.0	1.116668618299626	0.0
11	0.0	0.0	0.0	1.2469261780892955	0.0
12	0.0	0.0	0.0	1.4443199116746683	0.0
13	0.0	0.0	0.0	1.5026458566832277	0.0
14	0.0	0.0	0.0	1.525730886543212	0.0
15	0.0	0.0	0.0	1.565321155143668	0.0
16	0.0	0.0	0.0	1.6323458070559895	0.0
17	0.0	0.0	0.0	1.718440701026559	0.0
18	0.0	0.0	0.0	1.8335312846763356	0.0
19	0.0	0.0	0.0	1.906243552641117	0.0
20	0.0	0.0	0.0	1.957209053346493	0.0
21	0.0	0.0	0.0	2.012969995037276	0.0
22	0.0	0.0	0.0	2.0834518253344263	0.0
23	0.0	0.0	0.0	2.1519262617307082	0.0
24	0.0	0.0	0.0	2.2118135131066095	0.0
25	0.0	0.0	0.0	2.254860960091894	0.0
26	0.0	0.0	0.0	2.3013655854620074	0.0
27	0.0	0.0	0.0	2.3677211060740393	0.0
28	0.0	0.0	0.0	2.4466785995081883	0.0
29	0.0	0.0	0.0	2.5212867394904563	0.0
30	0.0	0.0	0.0	2.5987944484406453	0.0
31	0.0	0.0	0.0	2.6663767097698745	0.0
32	0.0	0.0	0.0	2.737862237017459	0.0
33	0.0	0.0	0.0	2.833659534842224	0.0
34	0.0	0.0	0.0	2.9283416138331746	0.0
35	0.0	0.0	0.0	3.061944830124291	0.0
36	0.0	0.0	0.0	3.1539503839140837	0.0
37	0.0	0.0	0.0	3.240045277884653	0.0
38	0.0	0.0	0.0	3.3407495385782076	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAT	20	7.855554E-4	44.000004	19
GTAATAC	20	7.855554E-4	44.000004	19
ACCGTCT	20	7.855554E-4	44.000004	14
TCGTTAG	25	4.4420543E-5	44.0	1
ACGTTAG	25	4.4420543E-5	44.0	1
TTATACG	30	2.527091E-6	44.0	1
CCCAACG	25	4.4420543E-5	44.0	15
TGCGATA	25	4.4420543E-5	44.0	34
ATAACGC	75	0.0	44.0	11
CGTTTTT	11875	0.0	43.407158	1
CGTAAGG	165	0.0	41.333332	2
TAACGGG	240	0.0	41.25	3
CGGTCTA	220	0.0	41.0	31
TAGGGTA	660	0.0	40.666668	5
ATTATGA	760	0.0	40.526314	26
ACGGGAT	210	0.0	39.80952	5
TAGTAAG	50	1.3478711E-9	39.600002	1
GTTTTTT	13235	0.0	39.478653	2
CTAGGCG	95	0.0	39.36842	4
AAGGGTA	370	0.0	39.24324	5
>>END_MODULE