FastQCFastQC Report
Thu 26 May 2016
SRR1546133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546133_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences594667
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCC409976.894110485364077No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332795.5962412577122995No Hit
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT295454.968326811476002No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA182133.062722498473936No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATG147242.4760075807132393No Hit
GAACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC65281.097757232198861No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTC50790.8540914495003086No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGT48490.815414341135459No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG41690.7010646294480777No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCGACACAAATCGTATGCC37540.6312776730506316No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCT37420.6292597369620309No Hit
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCCT34810.5853696270349624No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26670.4484862956915383No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACACAAATCGT17990.30252225194941035No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACACAAA17040.2865469245813203No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT12890.21675996818387436No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11440.19237657377994744No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGACACAAATCG10810.181782409314793No Hit
GAATGATACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG6950.1168721317981324No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGACACAAATCGTA6310.10610980599226122No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG6210.10442819258509384No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCT6140.10325106320007668No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGG302.5256468E-644.0000042
CGACGGT600.044.00000428
TTGTCCG207.8534114E-444.00000424
TACGGAC207.8534114E-444.00000429
TATGATC207.8534114E-444.0000042
GTAATAG207.8534114E-444.0000041
ACGGCTA2400.044.00000430
TCAGCCC207.8534114E-444.00000425
CGAATGT207.8534114E-444.00000422
ATTGCGG207.8534114E-444.0000042
GCGTATA207.8534114E-444.00000433
AGTACGG302.5256468E-644.0000042
ATCGAAT207.8534114E-444.00000443
ACCGGTG1650.044.017
TCACCTA254.4402404E-544.037
CGTTGAA351.444514E-744.019
ACAGACG254.4402404E-544.017
GGCATAG254.4402404E-544.01
CGATCCA351.444514E-744.034
GGTTAAC254.4402404E-544.08
Produced by FastQC (version 0.11.5)