FastQCFastQC Report
Thu 26 May 2016
SRR1546132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546132_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences604187
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCC394966.537048960007414No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343515.6854914124269476No Hit
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT298574.9416819627036No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA177832.9432940463796804No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATG149372.4722478305557716No Hit
GAACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC67351.114721104558688No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTC48830.8081934897639307No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGT47710.7896561826057166No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG44330.7337132377889627No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCT38810.6423507953663352No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCGACACAAATCGTATGCC36730.6079243677867945No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26760.4429092317444765No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACACAAA20350.33681625059791087No Hit
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCCT20090.3325129471504683No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACACAAATCGT18730.31000335988692246No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT12740.21086186892468722No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGACACAAATCG11600.191993538424362No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11100.18371795487158776No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGACACAAATCGTA6330.10476888777812168No Hit
GAATGATACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG6290.10410684109389973No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG6210.10278274772545586No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCT6110.10112763101490102No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC254.440325E-544.025
GCCCCTC302.525716E-644.035
CACGGGA351.4445504E-744.04
CGACGGT302.525716E-644.028
GCGAATC207.853513E-444.033
GTAGGGC900.044.04
TAGCCGT302.525716E-644.044
TGGCCCG207.853513E-444.033
ATACTCC254.440325E-544.028
CCCGTCG254.440325E-544.018
CGCTCAC254.440325E-544.034
CGGGATA254.440325E-544.06
TAGGGCA800.044.05
TCGTATA254.440325E-544.031
CGTAAGG207.853513E-444.02
TACTACG207.853513E-444.026
CTTTCAC207.853513E-444.030
CGGTCTA254.440325E-544.031
CCGCGTA302.525716E-644.031
CGCGTAT207.853513E-444.032
Produced by FastQC (version 0.11.5)