##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546132_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	604187
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.363738379011796	30.0	28.0	31.0	23.0	33.0
2	27.970494234400938	30.0	27.0	31.0	16.0	33.0
3	27.6355399901024	30.0	26.0	31.0	16.0	33.0
4	32.17717527851477	35.0	32.0	35.0	28.0	35.0
5	33.328560528445664	35.0	33.0	35.0	28.0	37.0
6	33.507559745575456	35.0	33.0	35.0	29.0	37.0
7	33.60283819413526	35.0	33.0	35.0	30.0	37.0
8	32.900371904724864	35.0	32.0	35.0	27.0	37.0
9	33.98945690655376	35.0	32.0	39.0	27.0	39.0
10	34.5745721109524	37.0	33.0	39.0	28.0	39.0
11	34.584686529170604	37.0	33.0	39.0	27.0	39.0
12	34.172377095170866	35.0	32.0	39.0	27.0	39.0
13	33.66283120954274	37.0	32.0	39.0	25.0	39.0
14	35.75404634657813	37.0	34.0	40.0	29.0	41.0
15	35.21082545635706	37.0	33.0	40.0	27.0	41.0
16	35.494489288912206	37.0	33.0	40.0	29.0	41.0
17	34.682775365904924	36.0	32.0	39.0	26.0	41.0
18	34.22393232558794	36.0	32.0	39.0	26.0	40.0
19	33.09726624372917	35.0	31.0	37.0	24.0	40.0
20	32.90195088606673	35.0	31.0	38.0	24.0	40.0
21	33.83939906022473	35.0	32.0	38.0	25.0	40.0
22	34.079458843040314	35.0	33.0	38.0	27.0	40.0
23	34.14742290052583	35.0	33.0	39.0	26.0	40.0
24	34.33478376727735	35.0	33.0	39.0	25.0	40.0
25	34.56263706435259	35.0	33.0	39.0	27.0	40.0
26	33.97373826315363	35.0	33.0	39.0	25.0	40.0
27	33.86322777550659	35.0	33.0	39.0	24.0	40.0
28	33.7688447450872	35.0	33.0	39.0	24.0	40.0
29	33.51321527937542	35.0	33.0	39.0	23.0	40.0
30	33.54550826151506	35.0	33.0	39.0	22.0	40.0
31	33.39994405705518	35.0	33.0	39.0	23.0	40.0
32	32.74198716622502	35.0	32.0	39.0	18.0	40.0
33	32.115482458245545	35.0	31.0	39.0	15.0	40.0
34	31.229965225997912	35.0	30.0	38.0	10.0	40.0
35	30.77120163128303	35.0	28.0	38.0	8.0	40.0
36	31.213306476306176	35.0	29.0	38.0	10.0	40.0
37	30.824123988103022	35.0	27.0	39.0	8.0	40.0
38	31.030133054832362	35.0	27.0	39.0	10.0	40.0
39	30.33621544323198	35.0	26.0	38.0	8.0	40.0
40	29.841314030258843	35.0	24.0	38.0	8.0	40.0
41	29.164387846809017	34.0	23.0	37.0	8.0	40.0
42	29.362538419396643	34.0	23.0	38.0	8.0	40.0
43	29.763958840557642	35.0	24.0	38.0	8.0	40.0
44	29.83062528654208	35.0	24.0	38.0	8.0	40.0
45	29.80145881986868	35.0	24.0	38.0	8.0	40.0
46	29.273911884896563	34.0	23.0	38.0	7.0	40.0
47	29.191293424055797	34.0	23.0	37.0	8.0	40.0
48	29.27590133518265	34.0	24.0	37.0	7.0	40.0
49	29.35921660015856	34.0	24.0	38.0	7.0	40.0
50	29.259658019785267	34.0	23.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	0.0
11	3.0
12	3.0
13	11.0
14	38.0
15	150.0
16	459.0
17	1414.0
18	3255.0
19	4810.0
20	5605.0
21	7501.0
22	9882.0
23	12782.0
24	15842.0
25	19224.0
26	22925.0
27	24531.0
28	23820.0
29	24920.0
30	28377.0
31	34621.0
32	41118.0
33	49530.0
34	60415.0
35	67949.0
36	50524.0
37	48571.0
38	39569.0
39	6336.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.80970130108725	16.781559351657684	11.236090812943674	24.172648534311396
2	30.262650470797947	49.83705375984587	11.71458505396508	8.185710715391096
3	13.653719792051136	48.5415939104946	29.794748976724094	8.009937320730172
4	13.845713330475498	17.50749354090704	59.29720434236421	9.349588786253262
5	35.971148005501604	19.763252105722234	29.767108527657825	14.49849136111833
6	10.581823177261345	47.40634935872503	33.83356477382002	8.1782626901936
7	33.47423893595857	12.077552148589758	49.20099240797965	5.247216507472024
8	27.786761383478957	34.75711989830963	29.341743533045232	8.114375185166182
9	26.204138784846414	12.32929540026515	26.834738251567806	34.63182756332063
10	30.40995585803733	18.205787281090128	33.31336159169263	18.070895269179907
11	27.73876299887287	18.73194888337551	37.57793530810825	15.951352809643371
12	19.388533682452618	16.97007714498988	38.18155637244761	25.4598328001099
13	22.482608861163843	20.85943590312271	46.72444127397644	9.933513961737011
14	11.4118642076046	38.717979698338425	31.510442958885243	18.359713135171727
15	8.563904883752878	21.469842945975333	48.5702274295872	21.396024740684588
16	9.344623436121598	24.452363258395167	32.57683465549574	33.6261786499875
17	9.269646649133463	30.16549511988838	40.04273511346653	20.522123117511633
18	9.491763311689924	25.72051368202229	38.57746028961232	26.210262716675466
19	11.059655371598529	29.93444082709492	30.964419956073204	28.041483845233344
20	19.147714201066886	25.797807632405203	40.62103289213439	14.433445274393526
21	12.692759029902994	39.375061032428704	34.76225076011235	13.169929177555955
22	9.891639508959974	21.22968551127383	41.231439934987016	27.647235044779183
23	18.118893653785996	32.19384064867334	36.65106995019754	13.036195747343124
24	17.47770144011705	29.110192705238607	31.53709033792518	21.875015516719163
25	12.315392419896488	44.32088078690869	29.330323227742404	14.033403565452417
26	12.67273211770528	21.306813949985685	45.44023621825693	20.580217714052107
27	23.8212672566606	24.86614243603388	30.362619520115462	20.949970787190058
28	11.997610011469959	29.27454579459671	40.0525003020588	18.675343891874537
29	25.571553178072353	22.390418860385942	36.84024979021396	15.19777817132775
30	12.734136947666865	41.66756318821822	31.90469175933941	13.693608104775508
31	15.977503653670139	22.93478674648743	26.666909417117544	34.42080018272489
32	26.550554712365543	31.65245197265085	27.2779785066544	14.519014808329208
33	18.410690729856817	24.388310241696693	26.01264178143522	31.18835724701127
34	22.84822414252541	29.852347038251402	27.210780768205872	20.08864805101732
35	15.890279003023899	38.357329767108524	27.07853694303254	18.673854286835038
36	17.497231817301596	33.16307699437426	25.06922525641896	24.27046593190519
37	11.25164891002289	37.42814724580304	33.37807665507533	17.942127189098738
38	13.133020074910581	26.907563386832223	23.93712542639944	36.022291111857754
39	17.504845354170147	37.13221237795583	26.389677368099612	18.97326489977441
40	12.82599592510266	31.961462262511443	32.34131154758378	22.871230264802122
41	12.47295953074131	38.20803823981648	26.437510241034644	22.88149198840756
42	14.234996780797998	27.90874348504685	39.66867873688113	18.187580997274022
43	19.679337688497103	27.571265187764716	26.158623075306153	26.590774048432024
44	20.462869939273766	24.287513634023902	30.30071815514071	24.94889827156162
45	14.929318240875755	23.481802819325807	34.354595514302694	27.234283425495747
46	24.896596583508085	31.67479604824334	25.72117572870651	17.70743163954206
47	13.081214921870215	26.306756020900814	41.72135448131125	18.89067457591772
48	21.02395450415186	25.81535186953708	27.866869032269808	25.293824594041247
49	15.43793560602926	22.799398199564042	35.90875010551369	25.85391608889301
50	16.32425060453138	21.54746791970036	33.93055461305854	28.197726862709725
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20417.0
1	14405.5
2	8394.0
3	25894.0
4	43394.0
5	26671.0
6	9948.0
7	9108.5
8	8269.0
9	8329.5
10	8390.0
11	8177.5
12	7965.0
13	7642.5
14	7320.0
15	6727.5
16	6135.0
17	5600.0
18	5065.0
19	4692.5
20	4320.0
21	4039.5
22	3759.0
23	3468.0
24	3177.0
25	3185.0
26	3193.0
27	3305.5
28	3418.0
29	3520.5
30	3623.0
31	4084.0
32	4545.0
33	5372.0
34	6199.0
35	7310.0
36	8421.0
37	9896.0
38	11371.0
39	13218.5
40	15066.0
41	18863.5
42	22661.0
43	71829.0
44	120997.0
45	91675.0
46	62353.0
47	54478.0
48	46603.0
49	41206.0
50	35809.0
51	32489.0
52	29169.0
53	26110.0
54	23051.0
55	20633.0
56	18215.0
57	16621.0
58	15027.0
59	13454.5
60	11882.0
61	10133.5
62	8385.0
63	7076.5
64	5768.0
65	4667.5
66	3567.0
67	3077.0
68	2587.0
69	2325.5
70	2064.0
71	1793.5
72	1523.0
73	1289.0
74	1055.0
75	789.0
76	523.0
77	400.0
78	277.0
79	205.0
80	133.0
81	99.0
82	65.0
83	52.0
84	39.0
85	29.5
86	20.0
87	13.0
88	6.0
89	6.0
90	6.0
91	5.0
92	4.0
93	4.5
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	604187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.2282944423318
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.35258521796733	25.25170186722545
2	7.437385861224081	4.793885228335303
3	3.3496955852790373	3.238649268436554
4	2.244552343650521	2.893523752895797
5	1.6416656390620163	2.6454041795774734
6	1.254210092121849	2.425263128486823
7	1.0055894813183759	2.268590372442823
8	0.771780851611752	1.9898544424557705
9	0.6539281391212907	1.8967489750554363
>10	3.1142925986840946	15.899892842408361
>50	0.08895679900595276	1.983481862941749
>100	0.06941715529365847	4.179911690949745
>500	0.006684614954204149	1.211400486097714
>1k	0.006170413803880753	5.597631411683801
>5k	5.14201150323396E-4	1.1161124861652671
>10k+	0.0025710057516169804	22.607948004841933
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCC	39496	6.537048960007414	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34351	5.6854914124269476	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT	29857	4.9416819627036	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17783	2.9432940463796804	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATG	14937	2.4722478305557716	No Hit
GAACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC	6735	1.114721104558688	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTC	4883	0.8081934897639307	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGT	4771	0.7896561826057166	No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG	4433	0.7337132377889627	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCT	3881	0.6423507953663352	No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCGACACAAATCGTATGCC	3673	0.6079243677867945	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2676	0.4429092317444765	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACACAAA	2035	0.33681625059791087	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCCT	2009	0.3325129471504683	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACACAAATCGT	1873	0.31000335988692246	No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT	1274	0.21086186892468722	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGACACAAATCG	1160	0.191993538424362	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1110	0.18371795487158776	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGACACAAATCGTA	633	0.10476888777812168	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG	629	0.10410684109389973	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG	621	0.10278274772545586	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCT	611	0.10112763101490102	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.106589516159732	0.0
2	1.6551167105548448E-4	0.0	0.0	1.1180313379797977	0.0
3	1.6551167105548448E-4	0.0	0.0	1.4897705511704158	0.0
4	1.6551167105548448E-4	0.0	0.0	2.9490869548666225	0.0
5	1.6551167105548448E-4	0.0	0.0	9.124658425288859	0.0
6	1.6551167105548448E-4	0.0	0.0	10.236069296426438	0.0
7	1.6551167105548448E-4	0.0	0.0	11.353604099393069	0.0
8	1.6551167105548448E-4	0.0	0.0	12.532047197308119	0.0
9	1.6551167105548448E-4	0.0	0.0	12.903124363814515	0.0
10	3.3102334211096895E-4	0.0	0.0	20.258794048862356	0.0
11	3.3102334211096895E-4	0.0	0.0	20.500772111945473	0.0
12	3.3102334211096895E-4	0.0	0.0	23.476671957523084	0.0
13	4.965350131664535E-4	0.0	0.0	23.68935445482938	0.0
14	6.620466842219379E-4	0.0	0.0	23.867776036227195	0.0
15	6.620466842219379E-4	0.0	0.0	24.285693005642294	0.0
16	9.93070026332907E-4	0.0	0.0	24.59453778383183	0.0
17	9.93070026332907E-4	0.0	0.0	24.71536130370233	0.0
18	9.93070026332907E-4	0.0	0.0	24.843963872112443	0.0
19	9.93070026332907E-4	0.0	0.0	25.346126282094783	0.0
20	9.93070026332907E-4	0.0	0.0	25.453874379951902	0.0
21	9.93070026332907E-4	0.0	0.0	25.50385890461066	0.0
22	0.0011585816973883913	0.0	0.0	25.644378313336766	0.0
23	0.0011585816973883913	0.0	0.0	25.70280393321935	0.0
24	0.0011585816973883913	0.0	0.0	25.89016314485416	0.0
25	0.0011585816973883913	0.0	0.0	25.95239553317102	0.0
26	0.0011585816973883913	0.0	0.0	26.089439196804964	0.0
27	0.0013240933684438758	0.0	0.0	26.327113956440638	0.0
28	0.0016551167105548449	0.0	0.0	26.411855932021048	0.0
29	0.0016551167105548449	0.0	0.0	26.520431588233443	0.0
30	0.0016551167105548449	0.0	0.0	26.598056561958465	0.0
31	0.0016551167105548449	0.0	0.0	26.669226580512326	0.0
32	0.0016551167105548449	0.0	0.0	26.794849938843438	0.0
33	0.0016551167105548449	0.0	0.0	26.849137766949635	0.0
34	0.0018206283816103292	0.0	0.0	26.94463800114865	0.0
35	0.0018206283816103292	0.0	0.0	27.03004202341328	0.0
36	0.0018206283816103292	0.0	0.0	27.075557732953538	0.0
37	0.0018206283816103292	0.0	0.0	27.130673119415015	0.0
38	0.0018206283816103292	0.0	0.0	27.1935675544161	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	25	4.440325E-5	44.0	25
GCCCCTC	30	2.525716E-6	44.0	35
CACGGGA	35	1.4445504E-7	44.0	4
CGACGGT	30	2.525716E-6	44.0	28
GCGAATC	20	7.853513E-4	44.0	33
GTAGGGC	90	0.0	44.0	4
TAGCCGT	30	2.525716E-6	44.0	44
TGGCCCG	20	7.853513E-4	44.0	33
ATACTCC	25	4.440325E-5	44.0	28
CCCGTCG	25	4.440325E-5	44.0	18
CGCTCAC	25	4.440325E-5	44.0	34
CGGGATA	25	4.440325E-5	44.0	6
TAGGGCA	80	0.0	44.0	5
TCGTATA	25	4.440325E-5	44.0	31
CGTAAGG	20	7.853513E-4	44.0	2
TACTACG	20	7.853513E-4	44.0	26
CTTTCAC	20	7.853513E-4	44.0	30
CGGTCTA	25	4.440325E-5	44.0	31
CCGCGTA	30	2.525716E-6	44.0	31
CGCGTAT	20	7.853513E-4	44.0	32
>>END_MODULE