FastQCFastQC Report
Thu 26 May 2016
SRR1546131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546131_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences601996
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCC401596.670974557970485No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338945.63026996857122No Hit
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT305405.07312340945787No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA174352.896198645838178No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATG146262.429584249729234No Hit
GAACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC65251.083894245144486No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTC50890.8453544541824198No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGT47500.7890417876530741No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG43070.71545325882564No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCT39270.6523299158133942No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCGACACAAATCGTATGCC37300.6196054458833614No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26280.43654775114784816No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACACAAA20610.34236107881115485No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACACAAATCGT20070.33339091954099365No Hit
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCCT12760.2119615412726995No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT11850.19684516176187217No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGACACAAATCG11790.1958484773985209No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10870.18056598382713507No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGACACAAATCGTA6550.10880470966584496No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACACCAATCGTATG6400.10631299875746682No Hit
GAATGATACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG6300.1046518581518814No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGG207.8534905E-444.03
GACGTCT207.8534905E-444.043
CCGTATA600.044.031
GCATTCC408.301868E-944.012
TGTTACG351.4445504E-744.026
ACTCGTA351.4445504E-744.040
TCGTATA502.7284841E-1144.031
ACCGTTG254.4403045E-544.017
ACGTAGG302.5256995E-644.02
AACTCGT351.4445504E-744.039
CGCACAC502.7284841E-1144.034
CACCGTT254.4403045E-544.016
GTTATTG302.5256995E-644.01
CGTTTTT96600.043.590061
CCGGTGA3300.043.33333218
CGATGAA35700.043.32212419
CCGATGA35750.043.2615418
CGGCTAT2800.043.21428731
ACCGGGC2600.043.15384730
ATACCGG2450.043.1020428
Produced by FastQC (version 0.11.5)