FastQCFastQC Report
Thu 26 May 2016
SRR1546130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546130_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences500162
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158643.171772345759974No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCC106602.1313094557363415No Hit
GAATGATACCTGTCCCTTATACACATCTGACGCGACACAAATCGTATGCC101422.0277430112643504No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCGACACAAATCGTATG90141.8022160819894353No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81491.6292721158344694No Hit
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT56871.1370316017610294No Hit
GAATCTTTCTCTTACCCACATCTGACGCGACACAAATCGTATGCCGTCTT56111.121836524965911No Hit
GAATGATACCTGTCTCTTATACACAACTGACGCGACACAAATCGTATGCC35890.7175675081273667No Hit
GAACTGTCCCTTATCCACATCTGACGCGACACAAATCGTATGCCGTCTTC34550.6907761885149212No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATG33730.6743815003938723No Hit
GAATGATACCTGGCCCTTATACACATCTGACGCGACACAAATCGTATGCC31490.6295960108924709No Hit
GAATCTGTCTCTTACCCACATCTGACGCGACACAAATCGTATGCCGTCTT23030.4604508139362846No Hit
GAATGACTCTCTCTCCTACACATCTGACGCGACACAAATCGTATGCCGTC21290.42566208548430307No Hit
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCCT19180.3834757538557507No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCGACACCAATCGTATG18390.36768087139766714No Hit
GCTGTCTCCTATACCCATCTGACGCGACACAAATCGTATGCCGTCTTCTG18150.36288242609394555No Hit
GAACTGTCCCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC18140.3626824908729571No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACACAAA17950.3588837216741776No Hit
GAATGCTGCCTCTTCCACACATCTGACGCGACACAAATCGTATGCCGTCT17950.3588837216741776No Hit
GAATGACTCTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTC16000.31989635358143964No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15950.31889667747649764No Hit
GAATGATCCGTCTCCCATACACATCTGACGCGACACAAATCGTATGCCGT15440.30869998120608927No Hit
GAATCTTTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT14130.2825084672566089No Hit
GAATGATACGGCGACCGTCTCTTATACACATCTGACGCGACACAAATCGT13110.26211507471579204No Hit
CGTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12330.2465201274786969No Hit
GAATGCTGCCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCT10760.2151302977835181No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACACCAATCGTATGCC10330.20653308328101697No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9930.19853567444148096No Hit
GAATCTTTCTCTTACCCCCATCTGACGCGACACAAATCGTATGCCGTCTT9760.19513677568467816No Hit
AAAAAAAACAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8890.17774241145868738No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACACCAATCGTATG8500.16994493784013978No Hit
GCTGTCTCCTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG7800.1559494723709518No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGACACAAATCG7770.1553496667079866No Hit
GAACTGTCCCTTATACACATCTGACGCGACACAAATCGTATGCCGTCCTC7730.154549925824033No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG7690.15375018494007942No Hit
CGCTGTCTCTTATACCCATCTGACGCGACACAAATCGTATGCCGTCTTCT7620.1523506383931606No Hit
CGTTTTTTCTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6860.13715556159804224No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCGACACAAATCGTATGCC6740.13475633894618144No Hit
GAATGATACGGCGCCCTCTCTTATACACATCTGACGCGACACAAATCGTA6350.12695886532763384No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCGACACAAA6230.12455964267577306No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT6030.12056093825600506No Hit
GAATGATCCGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGT5960.11916139170908624No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5640.11276346463745747No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCGACACACATCGTATG5610.11216365897449226No Hit
GAATGACTCGCTCTCCTACACATCTGACGCGACACAAATCGTATGCCGTC5370.10736521367077066No Hit
GAATGATACCTGTCCCTCATACACATCTGACGCGACACAAATCGTATGCC5190.10376637969297949No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCC207.85221E-444.0000044
TGACGGG408.296411E-944.0000043
ACGGGAT207.85221E-444.0000045
GGGACCA207.85221E-444.0000047
TAGGGTG207.85221E-444.0000045
AACTGCC207.85221E-444.0000044
ATTGGGT207.85221E-444.0000044
GTGACGG207.85221E-444.0000042
TTACCCG650.044.00000428
CGATCAC207.85221E-444.00000412
AATACCG207.85221E-444.00000427
TACCCGG502.7284841E-1144.029
TCACCTA254.4392225E-544.037
ATAGGGT351.4438956E-744.04
CGCATGG454.783942E-1044.02
CGTTGAA600.044.019
TGAACGA351.4438956E-744.022
CGTCTCA750.044.020
GATCAGT254.4392225E-544.025
ATAGAGG254.4392225E-544.02
Produced by FastQC (version 0.11.5)