##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546130_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	500162
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.747395843746627	16.0	16.0	28.0	16.0	31.0
2	21.542848117210024	16.0	16.0	30.0	10.0	31.0
3	22.325630495719388	25.0	16.0	30.0	10.0	31.0
4	26.66666600021593	28.0	19.0	33.0	19.0	35.0
5	30.74630819614445	33.0	28.0	35.0	19.0	35.0
6	31.098452101519108	33.0	30.0	35.0	19.0	35.0
7	29.91635310159508	32.0	28.0	35.0	17.0	35.0
8	29.988363770138474	32.0	28.0	35.0	17.0	35.0
9	23.67385966946709	25.0	17.0	31.0	16.0	35.0
10	25.741537741771666	27.0	17.0	34.0	17.0	37.0
11	27.73678528156877	31.0	17.0	35.0	17.0	39.0
12	27.876707946625295	31.0	17.0	37.0	15.0	39.0
13	28.066324510858482	31.0	17.0	37.0	11.0	39.0
14	30.912938208020602	32.0	27.0	38.0	17.0	40.0
15	28.240576053358712	30.0	17.0	37.0	16.0	40.0
16	29.421193533295213	31.0	25.0	36.0	17.0	40.0
17	28.061761989115528	31.0	17.0	36.0	16.0	40.0
18	28.294542568207902	31.0	17.0	36.0	16.0	39.0
19	27.249857046316993	30.0	18.0	36.0	10.0	37.0
20	27.07981613957078	31.0	18.0	35.0	10.0	38.0
21	29.46123056129814	33.0	25.0	36.0	15.0	38.0
22	30.792547214702438	33.0	27.0	36.0	17.0	38.0
23	30.651716843742626	34.0	27.0	36.0	16.0	39.0
24	31.945993498106613	35.0	30.0	37.0	19.0	39.0
25	31.661811573050333	34.0	30.0	37.0	20.0	39.0
26	25.24214354549126	32.0	15.0	36.0	0.0	38.0
27	27.732818566784363	33.0	18.0	36.0	10.0	39.0
28	29.013567604096274	33.0	23.0	36.0	13.0	39.0
29	29.333246028286837	33.0	25.0	36.0	13.0	38.0
30	29.709786029326498	33.0	26.0	36.0	11.0	38.0
31	29.40453493068246	33.0	25.0	36.0	12.0	38.0
32	28.865171684374264	33.0	23.0	36.0	9.0	39.0
33	28.7507487574026	33.0	24.0	36.0	9.0	38.0
34	27.062789656151406	32.0	19.0	35.0	8.0	38.0
35	26.21289302266066	31.0	16.0	35.0	7.0	38.0
36	27.83043293972753	33.0	21.0	35.0	8.0	38.0
37	27.229079778151878	33.0	18.0	36.0	7.0	38.0
38	27.549354009300988	32.0	21.0	36.0	8.0	38.0
39	26.41295420283828	31.0	18.0	35.0	7.0	38.0
40	25.906326350262514	30.0	17.0	35.0	7.0	38.0
41	24.173819682422895	27.0	14.0	34.0	7.0	37.0
42	24.679345891931014	28.0	14.0	34.0	7.0	37.0
43	25.379884917286798	29.0	16.0	34.0	7.0	37.0
44	25.732140786385212	30.0	18.0	35.0	7.0	37.0
45	25.604546127054835	30.0	18.0	35.0	7.0	37.0
46	24.164794606547478	27.0	14.0	33.0	7.0	36.0
47	24.41588525317797	28.0	15.0	33.0	7.0	36.0
48	24.625687277322147	28.0	15.0	33.0	7.0	36.0
49	24.613737149163672	28.0	15.0	34.0	7.0	36.0
50	25.663485030850005	29.0	17.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
9	1.0
10	0.0
11	7.0
12	55.0
13	383.0
14	1607.0
15	4361.0
16	8065.0
17	11329.0
18	13105.0
19	13794.0
20	14356.0
21	15612.0
22	17595.0
23	21296.0
24	26450.0
25	32928.0
26	38574.0
27	41050.0
28	39533.0
29	35985.0
30	33229.0
31	31784.0
32	29159.0
33	26039.0
34	21616.0
35	15146.0
36	5945.0
37	1127.0
38	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	55.12294016738577	13.66977099419788	8.734370064099233	22.472918774317122
2	28.423790691815853	55.17352377829583	9.346371775544723	7.056313754343593
3	11.828767479336696	53.189366645206945	28.083301010472606	6.898564864983745
4	12.68648957737693	13.85091230441337	65.2722517904199	8.190346327789797
5	42.1785341549338	16.058796949788267	28.70529948296752	13.05736941231041
6	9.210415825272612	51.22960160907866	32.79057585342349	6.769406712225239
7	29.77975136055918	7.705903287334904	58.582419296148046	3.9319260559578697
8	27.039639156913157	37.429472850796344	28.1040942734554	7.426793718835098
9	20.092490033229236	6.001655463629784	17.910996837024804	55.99485766611618
10	33.09567700065179	14.626261091406384	33.15725704871622	19.12080485922561
11	32.659218413234115	15.483583319004643	36.79887716379893	15.058321103962315
12	17.37776960264874	13.768139122924172	38.654875820234246	30.19921545419284
13	25.287007009728846	18.79630999556144	46.897805111143995	9.018877883565725
14	10.97864291969402	38.376366057397405	31.260871477641246	19.384119545267335
15	5.429240925939995	15.516572630467728	36.78208260523591	42.27210383835637
16	5.993857990011237	16.80855402849477	27.318948660633957	49.87863932086004
17	7.03911932533859	26.046360979042788	40.33313206521087	26.58138763040775
18	6.975939795506256	23.42900900108365	36.5253657814868	33.069685421923296
19	8.648797789516196	27.448706619055425	31.00395471867115	32.89854087275722
20	13.168333459958973	23.831878471375276	42.94508579220332	20.05470227646243
21	12.37019205777328	36.79047988451742	34.01537901719843	16.823949040510875
22	7.8542552213082955	19.263758542232317	43.077842778939626	29.804143457519768
23	15.785485502697128	32.122992150543226	37.4370703891939	14.654451957565747
24	16.682594839272074	25.720066698389722	32.02962240234164	25.56771605999656
25	10.721526225502936	43.979350690376315	28.87004610506196	16.429076979058785
26	10.454212835041446	32.2543495907326	37.34330077055034	19.94813680367561
27	19.53567044277654	25.89600969285951	30.986760289666147	23.581559574697796
28	11.060416425078275	27.27376330069058	41.618715536166285	20.047104738064867
29	25.802440009436943	21.889707734693957	35.403929126962865	16.903923128906236
30	10.637153562245832	41.54034092953883	32.677212583123065	15.14529292509227
31	16.53864148016043	21.642987671994273	24.80856202590361	37.00980882194169
32	21.36967622490313	33.69588253405896	25.585910165106508	19.3485310759314
33	13.86330828811465	23.259663868906475	24.695198755603183	38.18182908737569
34	23.082121392668775	29.527832982113793	25.10186699509359	22.288178630123838
35	12.431372235395733	38.049471971081374	26.16032405500618	23.35883173851672
36	17.32638625085472	30.53070805059161	23.397819106609457	28.745086591944208
37	9.693059448738609	36.783082281340846	32.10479804543328	21.419060224487264
38	12.89522194808882	24.75218029358488	21.593803607631127	40.758794150695174
39	16.024208156557275	35.416125175443156	25.649889435822793	22.909777232176776
40	11.065814676044962	29.89071540820774	30.884993262183052	28.158476653564247
41	11.753791771466044	36.2494551765228	25.00469847769323	26.99205457431792
42	12.431972041058698	26.055957869650232	37.5026491416781	24.009420947612973
43	19.885157209064264	26.275486742295495	23.181089327058032	30.658266721582205
44	18.891679095972904	22.53429888716056	29.578016722581886	28.99600529428465
45	13.749545147372253	21.004994381820293	31.553376705947272	33.692083764860186
46	23.711917338782236	29.126962864032052	24.36810473406616	22.793015063119547
47	10.998236571350882	22.080246000295904	42.531419819978325	24.390097608374887
48	19.829175347187512	24.53185168005566	26.05395851744035	29.58501445531648
49	12.817847017566308	20.27023244468792	35.66384491424778	31.248075623497982
50	15.76749133280817	19.437902119713215	32.37631007553553	32.418296471943094
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9513.0
1	8345.0
2	7177.0
3	16414.0
4	25651.0
5	18688.5
6	11726.0
7	9818.5
8	7911.0
9	8091.0
10	8271.0
11	7837.0
12	7403.0
13	7186.5
14	6970.0
15	6624.0
16	6278.0
17	5918.0
18	5558.0
19	5251.0
20	4944.0
21	4533.5
22	4123.0
23	3891.5
24	3660.0
25	3489.5
26	3319.0
27	3193.0
28	3067.0
29	3071.0
30	3075.0
31	3170.0
32	3265.0
33	3617.5
34	3970.0
35	4157.0
36	4344.0
37	4912.0
38	5480.0
39	6585.0
40	7690.0
41	12057.5
42	16425.0
43	30774.5
44	45124.0
45	53157.0
46	61190.0
47	56130.5
48	51071.0
49	47068.5
50	43066.0
51	37573.5
52	32081.0
53	26459.5
54	20838.0
55	18526.5
56	16215.0
57	15209.0
58	14203.0
59	13068.5
60	11934.0
61	10916.0
62	9898.0
63	9181.5
64	8465.0
65	7523.0
66	6581.0
67	5781.5
68	4982.0
69	4281.5
70	3581.0
71	3202.5
72	2824.0
73	2539.0
74	2254.0
75	1966.0
76	1678.0
77	1553.5
78	1429.0
79	1313.0
80	1197.0
81	1011.0
82	825.0
83	637.0
84	449.0
85	361.5
86	274.0
87	200.0
88	126.0
89	79.5
90	33.0
91	26.0
92	19.0
93	11.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	500162.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.65110052855254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.98304952184733	40.57854987523764
2	6.190810344177183	5.7761623143882534
3	2.2639728798612837	3.1685047923697605
4	1.2446708431592952	2.3226105851673013
5	0.7568001109621356	1.7652779028257153
6	0.5157081440168702	1.4435011481954558
7	0.36794784081943416	1.2015620197931889
8	0.2827515737269603	1.055253767243429
9	0.21264486523090484	0.8928105286290705
>10	1.0274623908373697	8.337810417743901
>50	0.07406300958442288	2.387510276069009
>100	0.058691441557460575	5.325884168214285
>500	0.008850296742791674	2.9481525362178465
>1k	0.009316101834517553	8.64410405677082
>5k	0.0018632203669035105	6.184666420969715
>10k+	0.001397415275177633	7.967639190164631
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15864	3.171772345759974	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCC	10660	2.1313094557363415	No Hit
GAATGATACCTGTCCCTTATACACATCTGACGCGACACAAATCGTATGCC	10142	2.0277430112643504	No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCGACACAAATCGTATG	9014	1.8022160819894353	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8149	1.6292721158344694	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT	5687	1.1370316017610294	No Hit
GAATCTTTCTCTTACCCACATCTGACGCGACACAAATCGTATGCCGTCTT	5611	1.121836524965911	No Hit
GAATGATACCTGTCTCTTATACACAACTGACGCGACACAAATCGTATGCC	3589	0.7175675081273667	No Hit
GAACTGTCCCTTATCCACATCTGACGCGACACAAATCGTATGCCGTCTTC	3455	0.6907761885149212	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATG	3373	0.6743815003938723	No Hit
GAATGATACCTGGCCCTTATACACATCTGACGCGACACAAATCGTATGCC	3149	0.6295960108924709	No Hit
GAATCTGTCTCTTACCCACATCTGACGCGACACAAATCGTATGCCGTCTT	2303	0.4604508139362846	No Hit
GAATGACTCTCTCTCCTACACATCTGACGCGACACAAATCGTATGCCGTC	2129	0.42566208548430307	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCCT	1918	0.3834757538557507	No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCGACACCAATCGTATG	1839	0.36768087139766714	No Hit
GCTGTCTCCTATACCCATCTGACGCGACACAAATCGTATGCCGTCTTCTG	1815	0.36288242609394555	No Hit
GAACTGTCCCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC	1814	0.3626824908729571	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACACAAA	1795	0.3588837216741776	No Hit
GAATGCTGCCTCTTCCACACATCTGACGCGACACAAATCGTATGCCGTCT	1795	0.3588837216741776	No Hit
GAATGACTCTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTC	1600	0.31989635358143964	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1595	0.31889667747649764	No Hit
GAATGATCCGTCTCCCATACACATCTGACGCGACACAAATCGTATGCCGT	1544	0.30869998120608927	No Hit
GAATCTTTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT	1413	0.2825084672566089	No Hit
GAATGATACGGCGACCGTCTCTTATACACATCTGACGCGACACAAATCGT	1311	0.26211507471579204	No Hit
CGTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1233	0.2465201274786969	No Hit
GAATGCTGCCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCT	1076	0.2151302977835181	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACACCAATCGTATGCC	1033	0.20653308328101697	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	993	0.19853567444148096	No Hit
GAATCTTTCTCTTACCCCCATCTGACGCGACACAAATCGTATGCCGTCTT	976	0.19513677568467816	No Hit
AAAAAAAACAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	889	0.17774241145868738	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACACCAATCGTATG	850	0.16994493784013978	No Hit
GCTGTCTCCTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG	780	0.1559494723709518	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGACACAAATCG	777	0.1553496667079866	No Hit
GAACTGTCCCTTATACACATCTGACGCGACACAAATCGTATGCCGTCCTC	773	0.154549925824033	No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG	769	0.15375018494007942	No Hit
CGCTGTCTCTTATACCCATCTGACGCGACACAAATCGTATGCCGTCTTCT	762	0.1523506383931606	No Hit
CGTTTTTTCTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	686	0.13715556159804224	No Hit
GAATGATACCTGGCTCTTATACACATCTGACGCGACACAAATCGTATGCC	674	0.13475633894618144	No Hit
GAATGATACGGCGCCCTCTCTTATACACATCTGACGCGACACAAATCGTA	635	0.12695886532763384	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCGACACAAA	623	0.12455964267577306	No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT	603	0.12056093825600506	No Hit
GAATGATCCGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGT	596	0.11916139170908624	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	564	0.11276346463745747	No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCGACACACATCGTATG	561	0.11216365897449226	No Hit
GAATGACTCGCTCTCCTACACATCTGACGCGACACAAATCGTATGCCGTC	537	0.10736521367077066	No Hit
GAATGATACCTGTCCCTCATACACATCTGACGCGACACAAATCGTATGCC	519	0.10376637969297949	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06577868770518353	0.0
2	0.0	0.0	0.0	0.4984385059240806	0.0
3	0.0	0.0	0.0	1.2865831470603524	0.0
4	0.0	0.0	0.0	1.5472986752292257	0.0
5	0.0	0.0	0.0	4.689880478724893	0.0
6	0.0	0.0	0.0	4.857226258692184	0.0
7	0.0	0.0	0.0	4.9735885573074325	0.0
8	0.0	0.0	0.0	5.144133300810537	0.0
9	0.0	0.0	0.0	5.43863788132645	0.0
10	0.0	0.0	0.0	9.635877975535927	0.0
11	0.0	0.0	0.0	9.818618767519323	0.0
12	0.0	0.0	0.0	11.468884081557576	0.0
13	0.0	0.0	0.0	11.574050007797474	0.0
14	0.0	0.0	0.0	11.637629408071785	0.0
15	0.0	0.0	0.0	11.795178362210644	0.0
16	0.0	0.0	0.0	12.115274651013072	0.0
17	0.0	0.0	0.0	12.2130429740764	0.0
18	0.0	0.0	0.0	12.326206309155834	0.0
19	0.0	0.0	0.0	13.038175631095525	0.0
20	0.0	0.0	0.0	13.155737541036704	0.0
21	0.0	0.0	0.0	13.199323419212176	0.0
22	0.0	0.0	0.0	13.329481248075624	0.0
23	0.0	0.0	0.0	13.383863628184468	0.0
24	0.0	0.0	0.0	13.54601109240606	0.0
25	0.0	0.0	0.0	13.594995221548219	0.0
26	0.0	0.0	0.0	13.71975479944498	0.0
27	0.0	0.0	0.0	13.965275250818735	0.0
28	0.0	0.0	0.0	14.051047460622758	0.0
29	0.0	0.0	0.0	14.174207556751613	0.0
30	0.0	0.0	0.0	14.231788900396271	0.0
31	0.0	0.0	0.0	14.288770438377966	0.0
32	0.0	0.0	0.0	14.404532931330248	0.0
33	0.0	0.0	0.0	14.45811557055514	0.0
34	0.0	0.0	0.0	14.531291861436895	0.0
35	0.0	0.0	0.0	14.596270808258124	0.0
36	0.0	0.0	0.0	14.629859925384176	0.0
37	0.0	0.0	0.0	14.672046257012727	0.0
38	0.0	0.0	0.0	14.714632459083257	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGCC	20	7.85221E-4	44.000004	4
TGACGGG	40	8.296411E-9	44.000004	3
ACGGGAT	20	7.85221E-4	44.000004	5
GGGACCA	20	7.85221E-4	44.000004	7
TAGGGTG	20	7.85221E-4	44.000004	5
AACTGCC	20	7.85221E-4	44.000004	4
ATTGGGT	20	7.85221E-4	44.000004	4
GTGACGG	20	7.85221E-4	44.000004	2
TTACCCG	65	0.0	44.000004	28
CGATCAC	20	7.85221E-4	44.000004	12
AATACCG	20	7.85221E-4	44.000004	27
TACCCGG	50	2.7284841E-11	44.0	29
TCACCTA	25	4.4392225E-5	44.0	37
ATAGGGT	35	1.4438956E-7	44.0	4
CGCATGG	45	4.783942E-10	44.0	2
CGTTGAA	60	0.0	44.0	19
TGAACGA	35	1.4438956E-7	44.0	22
CGTCTCA	75	0.0	44.0	20
GATCAGT	25	4.4392225E-5	44.0	25
ATAGAGG	25	4.4392225E-5	44.0	2
>>END_MODULE