FastQCFastQC Report
Thu 26 May 2016
SRR1546129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546129_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences872080
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432004.953673974864691No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT139901.604210622878635No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC113981.3069901843867535No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88201.0113751032015412No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATG81310.9323685900376112No Hit
GAACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC38180.43780387120447667No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG26100.2992844693147418No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTC22470.25765984772039263No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGT21980.2520410971470507No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCCT15200.17429593615264655No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14820.16993853774883036No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT14620.1676451701678745No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT14330.1643197871754885No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT12850.147348867076415No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10890.12487386478304743No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCCAGA10170.11661774149160627No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT10090.11570039445922392No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT9470.10859095495826072No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGT9420.10801761306302174No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG207.8554347E-444.0000041
TCGAGTA207.8554347E-444.00000426
GCGTAAG207.8554347E-444.0000041
CATCGTT302.527011E-644.036
CGTTTTT206500.043.4140431
CGGTCTA1250.042.2431
TACGGCT10250.041.424397
CGGCTGT10300.041.2232979
CGATGAA6050.040.72727219
TCACGAC1300.040.61538325
CATATGC5100.040.11764533
ACGGCTG10600.040.0566028
GTTTTTT228550.039.5720862
GATACCT14750.039.5254255
ACGGGAT1400.039.285715
TGATACC15000.039.164
GAATCTG19550.038.9360581
CGAAAGG850.038.8235322
TATGGGA4250.038.8235324
AGGGCGC2500.038.726
Produced by FastQC (version 0.11.5)