##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546129_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	872080
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.88392234657371	31.0	28.0	31.0	23.0	33.0
2	28.926400100908175	31.0	28.0	31.0	23.0	33.0
3	28.353798963397853	31.0	28.0	31.0	16.0	34.0
4	31.90608889092744	35.0	32.0	35.0	25.0	37.0
5	33.638756765434366	35.0	33.0	35.0	30.0	37.0
6	33.66974589487203	35.0	33.0	37.0	28.0	37.0
7	33.745651775066506	35.0	33.0	37.0	29.0	37.0
8	34.0497351160444	35.0	35.0	37.0	31.0	37.0
9	35.7601515915971	38.0	35.0	39.0	30.0	39.0
10	35.83001559489955	37.0	35.0	39.0	30.0	39.0
11	35.954163608843224	37.0	35.0	39.0	30.0	39.0
12	35.30304903219888	37.0	34.0	39.0	29.0	39.0
13	35.60743624438125	37.0	35.0	39.0	30.0	39.0
14	36.82205302265847	38.0	35.0	40.0	31.0	41.0
15	36.84784308779011	39.0	35.0	41.0	31.0	41.0
16	36.63198674433538	38.0	35.0	41.0	31.0	41.0
17	36.169328501972295	38.0	34.0	40.0	29.0	41.0
18	35.56080634804147	38.0	34.0	39.0	28.0	40.0
19	34.77938835886616	37.0	33.0	39.0	27.0	40.0
20	34.769137005779285	35.0	34.0	39.0	29.0	40.0
21	35.21284285845335	35.0	34.0	39.0	30.0	40.0
22	35.502031923676725	36.0	34.0	39.0	30.0	40.0
23	35.654558068067146	36.0	35.0	39.0	31.0	41.0
24	35.44968580864141	36.0	34.0	40.0	30.0	41.0
25	35.212635308687275	35.0	34.0	39.0	30.0	41.0
26	35.05904045500413	35.0	34.0	39.0	29.0	41.0
27	35.337483946426936	36.0	34.0	40.0	30.0	41.0
28	35.22710875149069	36.0	34.0	40.0	30.0	41.0
29	35.19463122649298	36.0	34.0	40.0	29.0	41.0
30	34.69449018438675	36.0	34.0	40.0	25.0	41.0
31	34.03436152646546	35.0	33.0	39.0	24.0	40.0
32	33.63365631593432	35.0	33.0	39.0	21.0	40.0
33	32.96699385377488	35.0	33.0	40.0	16.0	41.0
34	32.24475621502614	35.0	31.0	40.0	12.0	41.0
35	31.811480598110265	35.0	31.0	40.0	10.0	41.0
36	31.4551543436382	35.0	30.0	40.0	8.0	40.0
37	31.307854783964775	35.0	29.0	40.0	8.0	40.0
38	31.103615493991377	35.0	28.0	40.0	8.0	40.0
39	30.80969291808091	35.0	27.0	39.0	8.0	40.0
40	30.49273117145216	35.0	25.0	39.0	8.0	40.0
41	29.99431015503165	35.0	24.0	39.0	7.0	40.0
42	30.28318388221264	35.0	25.0	39.0	7.0	40.0
43	30.214558297403908	35.0	25.0	39.0	7.0	40.0
44	30.1482536005871	35.0	24.0	39.0	7.0	40.0
45	30.111646867259886	35.0	24.0	39.0	7.0	40.0
46	29.792013347399322	35.0	23.0	38.0	7.0	40.0
47	29.742674983946426	35.0	23.0	38.0	7.0	40.0
48	29.711209980735713	35.0	23.0	38.0	7.0	40.0
49	29.580199064306026	35.0	24.0	38.0	7.0	40.0
50	29.68220461425557	35.0	23.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	6.0
12	13.0
13	12.0
14	48.0
15	116.0
16	336.0
17	871.0
18	1915.0
19	3636.0
20	5003.0
21	6752.0
22	9189.0
23	12785.0
24	18483.0
25	27318.0
26	37232.0
27	39868.0
28	35567.0
29	31927.0
30	32614.0
31	36662.0
32	44325.0
33	57696.0
34	78239.0
35	84888.0
36	80588.0
37	101026.0
38	103835.0
39	21124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.14140904504174	17.741835611411798	18.09352352995138	33.02323181359508
2	45.939134024401426	24.76859921108155	19.110632052105313	10.181634712411705
3	22.34600036693881	23.93553343729933	43.21484267498394	10.50362352077791
4	19.772727272727273	19.518278139620218	48.487180075222454	12.221814512430052
5	20.57999266122374	22.89709659664251	42.721195303183194	13.801715438950554
6	14.50681130171544	34.316691129254195	42.59895881111825	8.577538757912118
7	54.065911384276674	7.024470232088799	35.21465920557747	3.694959178057059
8	52.45929272543803	11.167553435464637	31.58689569764242	4.786258141454913
9	49.79153288689111	7.36170993486836	31.289216585634342	11.557540592606182
10	27.774516099440415	23.66996147142464	36.945807724062014	11.60971470507293
11	23.482134666544354	19.92305751765893	41.98250160535731	14.61230621043941
12	19.40223374002385	18.290179800018347	45.98798275387579	16.319603706082013
13	17.513760205485735	22.151866801210897	47.08283643702413	13.25153655627924
14	13.733029079900927	28.79632602513531	42.928171727364465	14.542473167599304
15	12.759609210164205	21.90521511787909	50.12086047151637	15.214315200440327
16	14.242500688010274	20.610035776534264	46.29449133107054	18.85297220438492
17	13.801715438950554	20.818961563159345	44.312907072745624	21.066415925144483
18	14.878795523346483	22.59574809650491	44.704614255572885	17.820842124575726
19	17.169525731584258	23.421819099165216	41.04520227502064	18.363452894229887
20	18.81799834877534	22.512269516558113	43.49623887716723	15.173493257499313
21	18.028850564168426	24.478488212090635	43.52800201816347	13.96465920557747
22	16.187735070177048	19.159366113200623	43.67064948169892	20.9822493349234
23	15.840404550041281	25.289766993853775	43.26220071553068	15.607627740574259
24	16.583570314650032	23.59806439776167	43.99883038253371	15.819534905054583
25	16.65179800018347	25.83375378405651	41.93640491698009	15.578043298779928
26	14.250412806164572	21.927116778277224	43.943674892211725	19.878795523346483
27	17.237753417117695	22.172965782955693	43.471585175671954	17.117695624254655
28	13.874988533162094	22.12537840565086	47.09636730575177	16.90326575543528
29	16.114920649481697	21.558572608017613	45.76334739932116	16.563159343179525
30	16.859462434639024	23.20062379598202	44.412095220621964	15.527818548756994
31	19.777199339510137	21.021924594073937	38.98140078891845	20.21947527749748
32	19.99529859645904	23.476286579212914	38.55208237776351	17.976332446564534
33	18.23640033024493	21.80361893404275	36.93560223832676	23.024378497385563
34	16.788253371250345	24.924777543344646	38.480299972479585	19.80666911292542
35	20.46303091459499	23.68830841207229	35.81013209797266	20.03852857536006
36	20.286785615998532	26.2708696449867	34.4855976515916	18.956747087423174
37	17.507453444638106	26.093362994220715	35.398128612053945	21.00105494908724
38	18.2744702320888	27.9392945601321	31.486904871112742	22.29933033666636
39	18.730850380699017	26.305499495459134	32.39289973396936	22.570750389872487
40	20.002637372718098	23.5472663058435	36.19622053022658	20.253875791211815
41	17.55676084762866	27.22708925786625	34.04550041280616	21.170649481698927
42	19.84565636180167	25.059054215209613	34.368635904962844	20.72665351802587
43	19.721241170534814	25.126823227226858	32.34714705072929	22.804788551509038
44	19.04790844876617	24.4517704797725	34.47424548206586	22.026075589395468
45	19.134368406568207	25.0909320245849	32.66856251720026	23.106137051646638
46	22.07274561966792	25.6082010824695	32.126066415925145	20.192986881937436
47	18.27080084395927	25.111801669571598	36.421773231813596	20.195624254655538
48	18.586138886340702	27.012774057425926	32.64585817814879	21.75522887808458
49	18.16152187872672	24.734772039262452	36.08912026419595	21.01458581781488
50	17.919342262177782	24.120952206219613	36.71291624621594	21.24678928538666
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11018.0
1	8401.5
2	5785.0
3	33084.0
4	60383.0
5	40151.0
6	19919.0
7	19159.0
8	18399.0
9	18734.0
10	19069.0
11	19087.5
12	19106.0
13	18384.5
14	17663.0
15	16692.5
16	15722.0
17	14334.5
18	12947.0
19	11955.5
20	10964.0
21	10081.5
22	9199.0
23	8626.5
24	8054.0
25	7685.5
26	7317.0
27	7830.5
28	8344.0
29	9281.0
30	10218.0
31	11922.5
32	13627.0
33	14260.5
34	14894.0
35	16732.5
36	18571.0
37	20650.5
38	22730.0
39	25437.0
40	28144.0
41	32762.0
42	37380.0
43	41457.5
44	45535.0
45	46553.0
46	47571.0
47	68171.5
48	88772.0
49	79705.0
50	70638.0
51	63111.5
52	55585.0
53	46761.5
54	37938.0
55	34376.5
56	30815.0
57	28677.5
58	26540.0
59	24160.0
60	21780.0
61	19138.0
62	16496.0
63	14822.5
64	13149.0
65	10810.5
66	8472.0
67	7395.0
68	6318.0
69	5602.5
70	4887.0
71	4209.5
72	3532.0
73	2850.5
74	2169.0
75	1690.5
76	1212.0
77	932.0
78	652.0
79	474.5
80	297.0
81	206.5
82	116.0
83	94.5
84	73.0
85	54.5
86	36.0
87	27.0
88	18.0
89	14.5
90	11.0
91	9.0
92	7.0
93	5.0
94	3.0
95	3.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	872080.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.448797719280186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.9784255178391	27.64241432649835
2	7.362748631975172	5.220011738255896
3	3.283531524077863	3.491917344057479
4	2.0359954005243175	2.886943564422855
5	1.4694616887245513	2.6045325079914248
6	1.1622205982016767	2.4719593734499234
7	0.8863290347982173	2.1993509067100803
8	0.7549916729030607	2.141083767398524
9	0.6028450152252978	1.923311790071817
>10	4.218511691096122	28.320654996524254
>50	0.1841917162887263	4.15611382368748
>100	0.050352212800027225	3.4678221731805485
>500	0.004872794787098369	1.1856510956718753
>1k	0.0038982358296786946	2.4378626355257964
>5k	6.497059716131159E-4	1.9520174555879912
>10k+	9.745589574196736E-4	7.898352500965674
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43200	4.953673974864691	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT	13990	1.604210622878635	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC	11398	1.3069901843867535	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8820	1.0113751032015412	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATG	8131	0.9323685900376112	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	3818	0.43780387120447667	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	2610	0.2992844693147418	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTC	2247	0.25765984772039263	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGT	2198	0.2520410971470507	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCCT	1520	0.17429593615264655	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1482	0.16993853774883036	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1462	0.1676451701678745	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1433	0.1643197871754885	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1285	0.147348867076415	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1089	0.12487386478304743	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCCAGA	1017	0.11661774149160627	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT	1009	0.11570039445922392	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT	947	0.10859095495826072	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGT	942	0.10801761306302174	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1466837904779378E-4	0.0	0.0	0.07419044124392257	0.0
2	1.1466837904779378E-4	0.0	0.0	0.5686404916980093	0.0
3	1.1466837904779378E-4	0.0	0.0	0.7423630859554169	0.0
4	1.1466837904779378E-4	0.0	0.0	1.3784285845335291	0.0
5	1.1466837904779378E-4	0.0	0.0	3.5560957710301806	0.0
6	1.1466837904779378E-4	0.0	0.0	3.9684432620860473	0.0
7	1.1466837904779378E-4	0.0	0.0	4.428492798825796	0.0
8	1.1466837904779378E-4	0.0	0.0	4.953100632969452	0.0
9	2.2933675809558757E-4	0.0	0.0	5.113980368773507	0.0
10	2.2933675809558757E-4	0.0	0.0	6.684707824970186	0.0
11	2.2933675809558757E-4	0.0	0.0	6.863475827905697	0.0
12	2.2933675809558757E-4	0.0	0.0	8.051325566461793	0.0
13	4.5867351619117514E-4	0.0	0.0	8.176543436381984	0.0
14	4.5867351619117514E-4	0.0	0.0	8.265182093385928	0.0
15	4.5867351619117514E-4	0.0	0.0	8.442459407393818	0.0
16	4.5867351619117514E-4	0.0	0.0	8.575704063847354	0.0
17	4.5867351619117514E-4	0.0	0.0	8.659870654068435	0.0
18	4.5867351619117514E-4	0.0	0.0	8.769493624438125	0.0
19	4.5867351619117514E-4	0.0	0.0	8.97188331345748	0.0
20	5.733418952389689E-4	0.0	0.0	9.044124392257592	0.0
21	5.733418952389689E-4	0.0	0.0	9.101802586918632	0.0
22	5.733418952389689E-4	0.0	0.0	9.21199889918356	0.0
23	5.733418952389689E-4	0.0	0.0	9.285960003669388	0.0
24	5.733418952389689E-4	0.0	0.0	9.37780937528667	0.0
25	5.733418952389689E-4	0.0	0.0	9.43766626914962	0.0
26	5.733418952389689E-4	0.0	0.0	9.52343821667737	0.0
27	5.733418952389689E-4	0.0	0.0	9.632373176772774	0.0
28	6.880102742867626E-4	0.0	0.0	9.700830199064306	0.0
29	6.880102742867626E-4	0.0	0.0	9.77031923676727	0.0
30	8.026786533345565E-4	0.0	0.0	9.848408402898817	0.0
31	8.026786533345565E-4	0.0	0.0	9.919273461150354	0.0
32	9.173470323823503E-4	0.0	0.0	9.992546555361894	0.0
33	9.173470323823503E-4	0.0	0.0	10.068801027428677	0.0
34	9.173470323823503E-4	0.0	0.0	10.151476928722136	0.0
35	9.173470323823503E-4	0.0	0.0	10.256054490413723	0.0
36	9.173470323823503E-4	0.0	0.0	10.326116870011926	0.0
37	9.173470323823503E-4	0.0	0.0	10.399733969360609	0.0
38	0.001032015411430144	0.0	0.0	10.48745527933217	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	20	7.8554347E-4	44.000004	1
TCGAGTA	20	7.8554347E-4	44.000004	26
GCGTAAG	20	7.8554347E-4	44.000004	1
CATCGTT	30	2.527011E-6	44.0	36
CGTTTTT	20650	0.0	43.414043	1
CGGTCTA	125	0.0	42.24	31
TACGGCT	1025	0.0	41.42439	7
CGGCTGT	1030	0.0	41.223297	9
CGATGAA	605	0.0	40.727272	19
TCACGAC	130	0.0	40.615383	25
CATATGC	510	0.0	40.117645	33
ACGGCTG	1060	0.0	40.056602	8
GTTTTTT	22855	0.0	39.572086	2
GATACCT	1475	0.0	39.525425	5
ACGGGAT	140	0.0	39.28571	5
TGATACC	1500	0.0	39.16	4
GAATCTG	1955	0.0	38.936058	1
CGAAAGG	85	0.0	38.823532	2
TATGGGA	425	0.0	38.823532	4
AGGGCGC	250	0.0	38.72	6
>>END_MODULE