FastQCFastQC Report
Thu 26 May 2016
SRR1546128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546128_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences873143
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440725.047512263168805No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT140571.6099310193175689No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC110161.261649008238055No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84360.966164763389273No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATG79810.9140541698209801No Hit
GAACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC38120.4365836982029289No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG25690.294224428300977No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTC22810.26124014050390376No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGT21270.2436027088346353No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT15070.17259486704926913No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14610.16732654330390326No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT14250.16320350732926908No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT13350.15289591739268368No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCCAGA11850.13571660083170797No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT10810.12380560801609816No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGT10760.12323296413073231No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT9800.11223820153170787No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCCT9290.10639723390097613No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9080.10399212958243952No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAA8750.10021267993902487No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATG207.8554417E-444.01
TTGTACG408.309144E-944.01
TCGACTA207.8554417E-444.016
TGCGTAG254.4419583E-544.01
CGTTTTT208950.043.4630281
GATACCT14200.039.9718325
AGGGTAC3800.039.947376
TACGGCT10600.039.849067
GTTTTTT231050.039.848522
CGATGAA6700.039.73134219
CGGTCTA950.039.3684231
CCGATGA6850.039.18248418
ACGGCTG10650.039.042258
GCGCGAC3900.038.9230779
GCCAGTA6000.038.50000425
GCGACGT404.1230305E-738.517
CGACGTC404.1230305E-738.518
TATGGGC1600.038.54
GACGTCG404.1230305E-738.519
ACGGGAG2000.038.55
Produced by FastQC (version 0.11.5)