##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546128_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	873143
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.86536226024832	31.0	28.0	31.0	23.0	33.0
2	28.90538434139654	31.0	28.0	31.0	23.0	33.0
3	28.37047081634967	31.0	28.0	31.0	16.0	34.0
4	31.89389252390502	35.0	32.0	35.0	25.0	37.0
5	33.67296307706756	35.0	33.0	35.0	30.0	37.0
6	33.75964418199539	35.0	33.0	37.0	30.0	37.0
7	33.82696877831008	35.0	33.0	37.0	30.0	37.0
8	34.106741965519966	35.0	35.0	37.0	31.0	37.0
9	35.88518948213523	38.0	35.0	39.0	30.0	39.0
10	35.8347590257266	37.0	35.0	39.0	30.0	39.0
11	36.021513085485424	37.0	35.0	39.0	30.0	39.0
12	35.26317567683644	37.0	34.0	39.0	29.0	39.0
13	35.6062867136311	37.0	35.0	39.0	30.0	39.0
14	36.81829207815902	38.0	35.0	40.0	31.0	41.0
15	36.900088530744675	39.0	35.0	41.0	31.0	41.0
16	36.68825037823129	38.0	35.0	41.0	31.0	41.0
17	36.23465228490637	38.0	34.0	40.0	30.0	41.0
18	35.66412947249191	38.0	34.0	39.0	29.0	40.0
19	34.854984807757724	37.0	33.0	39.0	27.0	40.0
20	34.74506810453729	35.0	34.0	39.0	28.0	40.0
21	35.09320580935769	35.0	34.0	39.0	30.0	40.0
22	35.456473910917225	35.0	34.0	39.0	30.0	40.0
23	35.65431893744782	36.0	35.0	39.0	31.0	41.0
24	35.39161053802184	35.0	34.0	39.0	30.0	41.0
25	35.16149931912642	35.0	34.0	39.0	30.0	41.0
26	35.02165739174454	35.0	34.0	39.0	29.0	41.0
27	35.336846312688756	36.0	34.0	40.0	30.0	41.0
28	35.223563608710144	36.0	34.0	40.0	30.0	41.0
29	35.19083357479817	36.0	34.0	40.0	29.0	41.0
30	34.7223249799861	36.0	34.0	40.0	26.0	41.0
31	34.071900020958765	35.0	33.0	39.0	24.0	40.0
32	33.69760394345485	35.0	33.0	39.0	21.0	40.0
33	33.035720380281354	35.0	33.0	40.0	16.0	41.0
34	32.225468222272866	35.0	31.0	40.0	12.0	41.0
35	31.819554185282364	35.0	31.0	40.0	10.0	41.0
36	31.467878686538175	35.0	30.0	40.0	8.0	40.0
37	31.327979494767753	35.0	29.0	40.0	8.0	40.0
38	31.15716096905089	35.0	28.0	40.0	8.0	40.0
39	30.82598154025171	35.0	27.0	39.0	8.0	40.0
40	30.47376088452865	35.0	25.0	39.0	8.0	40.0
41	29.95232167010444	35.0	24.0	38.0	7.0	40.0
42	30.244792662828427	35.0	25.0	39.0	7.0	40.0
43	30.217009126798246	35.0	25.0	39.0	7.0	40.0
44	30.1424199701538	35.0	24.0	39.0	7.0	40.0
45	30.10203597807003	35.0	24.0	39.0	7.0	40.0
46	29.7794908737744	35.0	23.0	38.0	7.0	40.0
47	29.74012733309435	35.0	23.0	38.0	7.0	40.0
48	29.70251951856683	35.0	23.0	38.0	7.0	40.0
49	29.65216121528776	35.0	24.0	38.0	7.0	40.0
50	29.758977624512823	35.0	24.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	3.0
11	5.0
12	4.0
13	22.0
14	42.0
15	110.0
16	303.0
17	751.0
18	1738.0
19	3294.0
20	4758.0
21	6466.0
22	8999.0
23	12730.0
24	18375.0
25	27309.0
26	37232.0
27	40411.0
28	35843.0
29	32315.0
30	32552.0
31	36786.0
32	44384.0
33	58280.0
34	78338.0
35	85131.0
36	80579.0
37	103246.0
38	104254.0
39	18879.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.27964147911625	17.55119150013228	18.019270612030333	33.14989640872113
2	46.021098491312415	24.735925272263533	19.001011289101555	10.241964947322488
3	22.285123971674743	23.86859884348841	43.221213478204604	10.625063706632247
4	19.63126314933522	19.52875989385473	48.60395147186658	12.236025484943475
5	20.64003261779571	22.81046747210938	42.723585941821675	13.825913968273237
6	14.544696573184462	34.4405211975587	42.59428295250606	8.420499276750773
7	54.02184980009002	6.973313649654181	35.30097589970944	3.7038606505463596
8	52.53606797511977	11.198852879768834	31.602383572908447	4.662695572202949
9	49.85872875348024	7.273837160694181	31.341143432404543	11.52629065342103
10	27.930476451165504	23.251174206286944	36.95156463488799	11.86678470765957
11	23.753726480084016	19.677303717718633	41.97033017501142	14.598639627185925
12	19.45477430386546	17.822853759349844	45.82685768539632	16.895514251388374
13	17.774866201756183	21.970742478608887	47.04750539144218	13.206885928192747
14	13.754218953825434	28.776271469850872	43.06889020469728	14.40061937162641
15	12.722085614842014	21.804904809406935	50.032583437077314	15.440426138673736
16	14.238561152067874	20.436514980936686	46.38655981895291	18.93836404804253
17	13.86210506182836	20.892568571242055	44.314047069036796	20.931279297892786
18	14.890573479945438	22.459894885488403	44.80961308743242	17.839918547133745
19	17.095596024935205	23.27774488256792	41.222457260723615	18.40420183177326
20	19.07831821362595	22.0185009786484	43.529639474862655	15.373541332863002
21	18.02499705088399	24.489115757670852	43.60144901808753	13.88443817335763
22	16.191734916273738	18.9935669185918	43.89097776652851	20.923720398605955
23	15.872199628239589	25.217518779856217	43.263245539390454	15.647036052513736
24	16.46774926902008	23.732653185102553	44.11453793937534	15.685059606502028
25	16.744221736874714	25.73106581625232	42.02095189447776	15.5037605523952
26	14.24302777437373	21.78806907917718	44.120951550891434	19.84795159555766
27	17.28170528767911	22.02102061174401	43.58335347130997	17.113920629266914
28	13.896120108619092	22.18216260108596	47.12114739510023	16.800569895194716
29	16.10377681548154	21.456508269550348	45.97540150926022	16.464313405707884
30	16.836760988749838	23.241095673904503	44.40807519501387	15.514068142331784
31	19.77396600556839	20.86691412517766	39.16506230938116	20.194057559872782
32	20.018484944619612	23.421020382686457	38.7695944421475	17.790900230546427
33	18.304332738165456	21.715228776958643	37.09552730766896	22.88491117720694
34	16.70574006777813	24.76707709962744	38.61257548878019	19.914607343814243
35	20.457931862249367	23.491341051809382	36.07072381041823	19.980003275523025
36	20.341799682297175	26.138444676301592	34.52733401058017	18.99242163082107
37	17.481328946117646	26.04601995320354	35.45089406889822	21.02175703178059
38	18.22897280285131	27.964720555510382	31.466896029630888	22.339410612007427
39	18.68766055502936	26.250911935387442	32.51254376430894	22.548883745274257
40	19.928121739508878	23.529021019466455	36.224535958027495	20.31832128299717
41	17.60776871600643	26.99099689283428	34.215243093055776	21.185991298103517
42	19.801911027174242	25.100699427241587	34.395282330614805	20.70210721496937
43	19.64695359179424	25.074243279737686	32.475550969314305	22.803252159153768
44	19.04018013086058	24.464033955491825	34.55218675520504	21.943599158442545
45	19.231099602241557	25.112954006388417	32.80848612426601	22.847460267104015
46	22.12615802909718	25.623866880911834	32.17937955180308	20.070595538187902
47	18.372133774192772	25.108945499190856	36.32818450127871	20.19073622533766
48	18.467994360603015	27.04368013028794	32.81501426455918	21.67331124454986
49	18.2029747704557	24.70763666432646	36.27538673504798	20.814001830169857
50	18.01091001130399	23.967093591771334	36.841846066451886	21.180150330472785
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10467.0
1	8166.0
2	5865.0
3	33797.5
4	61730.0
5	40821.0
6	19912.0
7	19121.0
8	18330.0
9	18671.5
10	19013.0
11	19077.0
12	19141.0
13	18516.0
14	17891.0
15	16667.0
16	15443.0
17	14196.5
18	12950.0
19	12022.5
20	11095.0
21	10106.5
22	9118.0
23	8539.5
24	7961.0
25	7551.0
26	7141.0
27	7744.0
28	8347.0
29	9131.5
30	9916.0
31	11781.5
32	13647.0
33	14304.0
34	14961.0
35	16688.5
36	18416.0
37	20519.5
38	22623.0
39	25238.0
40	27853.0
41	32450.5
42	37048.0
43	41236.0
44	45424.0
45	46540.0
46	47656.0
47	67894.5
48	88133.0
49	79409.5
50	70686.0
51	63598.5
52	56511.0
53	47394.5
54	38278.0
55	34615.0
56	30952.0
57	28788.0
58	26624.0
59	24370.0
60	22116.0
61	19394.5
62	16673.0
63	14856.5
64	13040.0
65	10907.0
66	8774.0
67	7562.0
68	6350.0
69	5569.0
70	4788.0
71	4188.5
72	3589.0
73	2916.0
74	2243.0
75	1698.5
76	1154.0
77	932.5
78	711.0
79	508.0
80	305.0
81	220.0
82	135.0
83	101.5
84	68.0
85	52.0
86	36.0
87	24.5
88	13.0
89	11.0
90	9.0
91	7.0
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	873143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.340521706542255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.65989748042963	27.445412926349693
2	7.421837426766757	5.245832133661565
3	3.3944746524195017	3.5988751540841664
4	2.1232537900890045	3.001475866285543
5	1.5337414652329038	2.710161177214368
6	1.1378128635209201	2.412654012074645
7	0.9357550771190878	2.3149050830453968
8	0.7452508917628736	2.107004425373264
9	0.5894042438741769	1.8746868127107126
>10	4.212733846137398	28.147110151545203
>50	0.1842973097831996	4.180737000641691
>100	0.05112132063362574	3.5280388104999436
>500	0.005209816115527709	1.301941717015955
>1k	0.0035817485794253003	2.285244878753743
>5k	6.512270144409636E-4	1.8891618497658593
>10k+	9.768405216614455E-4	7.956758000978276
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44072	5.047512263168805	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT	14057	1.6099310193175689	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC	11016	1.261649008238055	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8436	0.966164763389273	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATG	7981	0.9140541698209801	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	3812	0.4365836982029289	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	2569	0.294224428300977	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTC	2281	0.26124014050390376	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGT	2127	0.2436027088346353	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1507	0.17259486704926913	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1461	0.16732654330390326	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1425	0.16320350732926908	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1335	0.15289591739268368	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCCAGA	1185	0.13571660083170797	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT	1081	0.12380560801609816	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGT	1076	0.12323296413073231	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT	980	0.11223820153170787	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCCT	929	0.10639723390097613	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	908	0.10399212958243952	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAA	875	0.10021267993902487	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1452877707317128E-4	0.0	0.0	0.06631216192536618	0.0
2	1.1452877707317128E-4	0.0	0.0	0.5192734752497586	0.0
3	1.1452877707317128E-4	0.0	0.0	0.6907230545282961	0.0
4	2.2905755414634257E-4	0.0	0.0	1.2996725622263479	0.0
5	2.2905755414634257E-4	0.0	0.0	3.409407164691236	0.0
6	2.2905755414634257E-4	0.0	0.0	3.8256047405751405	0.0
7	2.2905755414634257E-4	0.0	0.0	4.2825745610970944	0.0
8	2.2905755414634257E-4	0.0	0.0	4.800588219799048	0.0
9	2.2905755414634257E-4	0.0	0.0	4.9600122774849025	0.0
10	3.435863312195139E-4	0.0	0.0	6.481527080901983	0.0
11	3.435863312195139E-4	0.0	0.0	6.66488765299613	0.0
12	3.435863312195139E-4	0.0	0.0	7.841556308645892	0.0
13	3.435863312195139E-4	0.0	0.0	7.966965319541014	0.0
14	4.5811510829268514E-4	0.0	0.0	8.049311510256624	0.0
15	4.5811510829268514E-4	0.0	0.0	8.240345510414674	0.0
16	4.5811510829268514E-4	0.0	0.0	8.37778004290248	0.0
17	4.5811510829268514E-4	0.0	0.0	8.467914190459066	0.0
18	4.5811510829268514E-4	0.0	0.0	8.573280665366383	0.0
19	5.726438853658564E-4	0.0	0.0	8.79706989576736	0.0
20	6.871726624390278E-4	0.0	0.0	8.868535852661019	0.0
21	8.01701439512199E-4	0.0	0.0	8.926487413860043	0.0
22	8.01701439512199E-4	0.0	0.0	9.04410846791419	0.0
23	8.01701439512199E-4	0.0	0.0	9.120842748553216	0.0
24	8.01701439512199E-4	0.0	0.0	9.211778597549314	0.0
25	8.01701439512199E-4	0.0	0.0	9.268241284646386	0.0
26	8.01701439512199E-4	0.0	0.0	9.361124122852729	0.0
27	8.01701439512199E-4	0.0	0.0	9.46809400063907	0.0
28	8.01701439512199E-4	0.0	0.0	9.536238622997608	0.0
29	8.01701439512199E-4	0.0	0.0	9.602207198591755	0.0
30	9.162302165853703E-4	0.0	0.0	9.683179043982486	0.0
31	9.162302165853703E-4	0.0	0.0	9.753614241882486	0.0
32	9.162302165853703E-4	0.0	0.0	9.824851141221998	0.0
33	0.0010307589936585416	0.0	0.0	9.896431626892731	0.0
34	0.0011452877707317129	0.0	0.0	9.983129911137121	0.0
35	0.0012598165478048841	0.0	0.0	10.078875968770292	0.0
36	0.0012598165478048841	0.0	0.0	10.145417188249805	0.0
37	0.0012598165478048841	0.0	0.0	10.220204479678586	0.0
38	0.0013743453248780556	0.0	0.0	10.30014556607566	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATG	20	7.8554417E-4	44.0	1
TTGTACG	40	8.309144E-9	44.0	1
TCGACTA	20	7.8554417E-4	44.0	16
TGCGTAG	25	4.4419583E-5	44.0	1
CGTTTTT	20895	0.0	43.463028	1
GATACCT	1420	0.0	39.971832	5
AGGGTAC	380	0.0	39.94737	6
TACGGCT	1060	0.0	39.84906	7
GTTTTTT	23105	0.0	39.84852	2
CGATGAA	670	0.0	39.731342	19
CGGTCTA	95	0.0	39.36842	31
CCGATGA	685	0.0	39.182484	18
ACGGCTG	1065	0.0	39.04225	8
GCGCGAC	390	0.0	38.923077	9
GCCAGTA	600	0.0	38.500004	25
GCGACGT	40	4.1230305E-7	38.5	17
CGACGTC	40	4.1230305E-7	38.5	18
TATGGGC	160	0.0	38.5	4
GACGTCG	40	4.1230305E-7	38.5	19
ACGGGAG	200	0.0	38.5	5
>>END_MODULE