FastQCFastQC Report
Thu 26 May 2016
SRR1546127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546127_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences862946
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432305.009583450181124No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT142761.6543329478321933No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC111301.2897678417884781No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84450.978624386693953No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATG79420.9203356872851836No Hit
GAACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC38920.4510131572543357No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG25810.2990917160517576No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTC22880.2651382589408839No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGT21240.24613359352728906No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14090.16327788760826287No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT13780.1596855423166687No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT13700.15875848546722507No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT13240.15342790858292407No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCCAGA12450.14427322219466804No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGT10830.1255003209934341No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT10040.11634563460517808No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9930.11507093143719306No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT9780.11333269984448623No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA207.8553887E-444.00000431
GGATCGT254.4419146E-544.08
TACGGGT351.4455145E-744.04
TGCGACG254.4419146E-544.01
AATCGTA254.4419146E-544.013
CGTTTTT205850.043.4763221
GCCCGAA1850.041.6216225
CCGATGA9200.041.13043218
CGATGAA9150.041.11475419
GTTTGCG603.6379788E-1240.3333361
CTAGCGG603.6379788E-1240.3333362
CGTTCGA557.8216544E-1140.014
AGGGCGC1600.039.8750046
CCACCGA12700.039.84251815
GTTTTTT227650.039.7575232
CGGCTGT9550.039.623039
GTAGGGA5850.039.4871834
TACGGGA1450.039.4482734
ACCACCG14800.039.2432414
ACGGGAC1350.039.111115
Produced by FastQC (version 0.11.5)