FastQCFastQC Report
Thu 26 May 2016
SRR1546126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546126_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences713660
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202832.842109688086764No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCTGCCCAGATCGTATG46800.6557744584255808No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44510.6236863492419359No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC29460.41280161421405154No Hit
GAATGATACCTGTCCCTTATACACATCTGACGCTGCCCAGATCGTATGCC26920.3772104363422358No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT24150.3383964352773029No Hit
GAATCTTTCTCTTACCCACATCTGACGCTGCCCAGATCGTATGCCGTCTT21110.29579912002914555No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATG19640.275201076142701No Hit
GAACTGTCCCTTATCCACATCTGACGCTGCCCAGATCGTATGCCGTCTTC18020.252501191043354No Hit
CGTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10780.15105232183392653No Hit
GAACTGTCCCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC9870.13830115180898467No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCCAGA9530.13353697839307233No Hit
GAATGACTCTCTCTCCTACACATCTGACGCTGCCCAGATCGTATGCCGTC9350.13101476893758932No Hit
GAATCTGTCTCTTACCCACATCTGACGCTGCCCAGATCGTATGCCGTCTT9180.12863268222963317No Hit
GCTGTCTCCTATACCCATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG9110.12765182299694533No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCCT9070.12709133200683798No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT9000.12611047277415016No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT8460.11854384440770115No Hit
GAATGATACGGCGACCGTCTCTTATACACATCTGACGCTGCCCAGATCGT7400.10369083316985679No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG254.4411376E-544.01
CGGGTAT207.8544737E-444.06
CGAACGC254.4411376E-544.024
ATAGACG207.8544737E-444.01
TAACGTA207.8544737E-444.024
CGGTCTA750.044.031
ACGTAGT207.8544737E-444.035
GCGTAGC207.8544737E-444.039
CGACGGT850.043.99999628
CGTTTTT177850.043.4928321
TACGGCT11100.042.4144137
GCGGAAT1350.042.3703738
CCTTATC3650.042.191789
ACGGCTG11350.041.480188
TGATCCG2500.041.364
ACCCGTC1550.041.1612917
TGTACGG750.041.0666662
CGACCGT1300.040.61538712
GATACCT12000.040.3333325
CGCAAGA2900.040.20689420
Produced by FastQC (version 0.11.5)