##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546126_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	713660
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.192604321385534	28.0	16.0	30.0	16.0	33.0
2	25.530918084241797	30.0	16.0	31.0	16.0	33.0
3	25.91022896056946	30.0	16.0	31.0	16.0	33.0
4	26.88219880615419	28.0	19.0	35.0	10.0	35.0
5	31.323172098758512	33.0	32.0	35.0	25.0	35.0
6	30.72731552840288	35.0	29.0	35.0	17.0	35.0
7	30.300517052938375	32.0	28.0	35.0	17.0	35.0
8	31.97169520499958	35.0	32.0	35.0	27.0	35.0
9	26.629202981812067	30.0	17.0	34.0	10.0	37.0
10	30.417183252529217	34.0	27.0	37.0	17.0	39.0
11	32.6086231538828	35.0	31.0	37.0	17.0	39.0
12	32.465417705910376	35.0	30.0	39.0	17.0	39.0
13	33.01356248073312	35.0	31.0	39.0	21.0	39.0
14	33.779019421012805	36.0	31.0	39.0	25.0	41.0
15	31.779542078861084	34.0	27.0	39.0	17.0	40.0
16	30.856398004652075	34.0	27.0	38.0	17.0	40.0
17	30.990702855701596	33.0	26.0	38.0	17.0	40.0
18	31.648900036431915	34.0	27.0	38.0	17.0	39.0
19	31.038242300255025	35.0	27.0	37.0	17.0	39.0
20	31.153853375556988	34.0	28.0	36.0	18.0	39.0
21	32.38102177507496	35.0	31.0	37.0	23.0	39.0
22	32.909549365243954	35.0	31.0	37.0	25.0	39.0
23	33.58295546899084	35.0	32.0	38.0	26.0	39.0
24	33.55555866939439	35.0	33.0	38.0	25.0	40.0
25	32.6661869797943	35.0	31.0	37.0	22.0	39.0
26	25.960831488383825	33.0	18.0	36.0	0.0	39.0
27	29.295681416921223	33.0	22.0	37.0	12.0	39.0
28	30.778443516520472	33.0	27.0	36.0	17.0	39.0
29	31.72946361012247	34.0	30.0	37.0	21.0	39.0
30	31.560559650253623	34.0	30.0	37.0	19.0	39.0
31	30.471538267522348	33.0	27.0	36.0	16.0	38.0
32	30.51420844659922	34.0	27.0	37.0	15.0	39.0
33	29.994336238544964	34.0	26.0	37.0	12.0	39.0
34	29.072072135190428	33.0	24.0	37.0	8.0	39.0
35	28.111882408990276	33.0	21.0	36.0	7.0	39.0
36	28.125964745116722	33.0	21.0	36.0	7.0	39.0
37	28.179882577137572	33.0	21.0	37.0	7.0	39.0
38	27.882539304430683	33.0	21.0	36.0	7.0	38.0
39	27.617331782641592	33.0	20.0	36.0	7.0	39.0
40	27.28082560322843	32.0	18.0	36.0	7.0	38.0
41	26.251849620267354	31.0	17.0	35.0	7.0	38.0
42	26.659818400919207	31.0	18.0	35.0	7.0	38.0
43	26.511998710870724	31.0	18.0	35.0	7.0	38.0
44	26.471228596250317	31.0	18.0	35.0	7.0	38.0
45	26.119659221478013	31.0	18.0	35.0	7.0	38.0
46	25.4387705630132	30.0	15.0	35.0	7.0	38.0
47	25.44709525544377	30.0	17.0	34.0	7.0	37.0
48	25.445331110052408	30.0	17.0	34.0	7.0	37.0
49	25.191147044811256	29.0	15.0	34.0	7.0	37.0
50	26.59464871227195	31.0	18.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	9.0
11	17.0
12	29.0
13	119.0
14	432.0
15	1146.0
16	2452.0
17	4347.0
18	6450.0
19	8922.0
20	11928.0
21	15472.0
22	20352.0
23	26345.0
24	33523.0
25	41132.0
26	46553.0
27	47093.0
28	46637.0
29	47775.0
30	51915.0
31	56783.0
32	61378.0
33	62091.0
34	56140.0
35	41946.0
36	18984.0
37	3596.0
38	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.488804192472607	17.314127175405655	17.210856710478378	33.98621192164336
2	46.35989126474792	23.672757335425835	19.410223355659557	10.55712804416669
3	23.073172098758512	21.609169632598153	44.27864809573186	11.039010172911471
4	20.525039934983045	18.532354342403945	48.764257489560855	12.178348233052153
5	21.031443544545024	22.165036572037106	43.66449009332175	13.139029790096124
6	14.262113611523693	34.20802623097834	43.361264467673685	8.168595689824286
7	55.02942577698063	4.761230838214275	37.51268110865118	2.6966622761539107
8	54.88187652383488	10.218451363394333	31.130230081551442	3.7694420312193477
9	44.58887985875627	4.397332062887089	27.09273323431326	23.921054844043383
10	28.89639324047866	22.953927640613177	37.304458705826306	10.84522041308186
11	24.87346915898327	18.699240534708405	42.54715130454278	13.880139001765546
12	18.8919093125578	17.315528402880922	46.97362889891545	16.818933385645828
13	17.7148782333324	21.756018272006276	47.853459630636436	12.675643864024885
14	13.440293697278815	28.620211305103272	43.949219516296274	13.990275481321637
15	11.084550065857691	19.301488103578734	46.96185858812319	22.652103242440376
16	12.233976963820306	17.027716279460808	45.06025278143654	25.67805397528235
17	12.871815710562453	19.57262562004316	45.30700893983129	22.2485497295631
18	14.002466160356471	21.64349970574223	45.20093601995348	19.15309811394782
19	16.682593952302216	22.38951321357509	42.08937028837261	18.838522545750077
20	18.018664349970575	21.31056805761847	45.02564246279741	15.645125129613541
21	18.029313678782614	23.39139085839195	44.12633466917019	14.452960793655242
22	15.613037020429896	18.273827873216938	45.2083625255724	20.904772580780765
23	15.005464787153548	25.044839279208585	44.19303309699297	15.7566628366449
24	15.766471428971778	22.951825799400275	45.454277947482	15.827424824145952
25	16.344898130762548	24.465571840932657	43.249166269652214	15.940363758652579
26	11.458397556259284	34.031751814589576	36.85606591374044	17.6537847154107
27	15.872123980607011	22.27671440181599	44.89070425692907	16.960457360647926
28	13.228428103018244	21.44200319479864	48.799148053695035	16.530420648488075
29	15.556847798671637	20.832048874814337	46.59767956730095	17.01342375921307
30	15.910657736176892	22.443880839615503	45.698091528178686	15.94736989602892
31	20.310932376762043	20.012611047277414	39.44203121934815	20.23442535661239
32	19.237031639716392	23.348233052153684	38.724462629263236	18.690272678866688
33	17.186755597903762	21.168063223383683	37.508477426225376	24.13670375248718
34	16.834767256116358	23.839363282235237	38.468738615026766	20.85713084662164
35	19.43278311801138	23.039402516604547	35.882072695681416	21.64574166970266
36	19.876411736681334	24.698315724574726	34.97001373202926	20.45525880671468
37	17.23972199646891	24.98528711150968	34.95824342123701	22.816747470784406
38	17.95434800885576	27.738699100411964	30.92130706498893	23.38564582574335
39	17.67382226830704	25.154695513269626	32.57265364459266	24.598828573830676
40	19.201440461844577	22.408009416248635	36.1174789115265	22.273071210380294
41	17.145559510130877	25.54858055656755	34.060897346075166	23.24496258722641
42	19.491214303730068	24.010873525208083	32.88736933553793	23.61054283552392
43	18.91909312557801	24.40882212818429	31.89964408822128	24.77244065801642
44	18.513718016982878	23.547347476389316	34.264775943726704	23.674158562901102
45	19.034133901297537	24.75310371885772	31.371241207297594	24.84152117254715
46	21.330745733262336	23.99195695429196	31.63074853571729	23.046548776728415
47	17.634307653504468	23.665330829806912	35.71140318919374	22.988958327494885
48	17.441638875655073	26.597259199058378	32.37171762463918	23.589384300647367
49	17.585404814617604	23.866547095255445	35.266793711291086	23.28125437883586
50	17.603901017291147	23.563461592354905	36.41257741781801	22.42005997253594
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5634.0
1	5029.0
2	4424.0
3	19159.5
4	33895.0
5	25943.0
6	17991.0
7	16708.5
8	15426.0
9	16009.5
10	16593.0
11	17047.5
12	17502.0
13	17140.5
14	16779.0
15	16275.0
16	15771.0
17	14901.5
18	14032.0
19	13188.0
20	12344.0
21	11386.5
22	10429.0
23	9573.5
24	8718.0
25	8205.0
26	7692.0
27	7854.5
28	8017.0
29	8532.5
30	9048.0
31	10346.5
32	11645.0
33	11924.0
34	12203.0
35	13323.0
36	14443.0
37	15703.0
38	16963.0
39	18520.5
40	20078.0
41	23430.5
42	26783.0
43	29813.5
44	32844.0
45	35233.0
46	37622.0
47	43793.0
48	49964.0
49	54394.5
50	58825.0
51	53939.0
52	49053.0
53	44017.5
54	38982.0
55	34461.5
56	29941.0
57	27186.5
58	24432.0
59	22097.5
60	19763.0
61	17471.0
62	15179.0
63	13459.5
64	11740.0
65	10163.0
66	8586.0
67	7436.5
68	6287.0
69	5588.5
70	4890.0
71	4249.0
72	3608.0
73	3004.0
74	2400.0
75	1877.0
76	1354.0
77	1075.5
78	797.0
79	629.0
80	461.0
81	356.0
82	251.0
83	195.0
84	139.0
85	108.5
86	78.0
87	55.0
88	32.0
89	21.0
90	10.0
91	8.5
92	7.0
93	4.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	713660.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.54377790364496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.42604010160001	44.360631003826946
2	6.64263634862513	6.980584179936761
3	2.683987517804735	4.230805320950621
4	1.4915282490927069	3.134821162293585
5	0.9100740455422437	2.390936426242167
6	0.6735119739185682	2.123331814381366
7	0.49471297853220547	1.8195862209032818
8	0.3784131511466696	1.5906605255735233
9	0.2918031720415243	1.379919695699606
>10	1.9076509589868018	17.631961603288566
>50	0.06036253989651961	2.135797323865194
>100	0.03061448572182035	3.260755100569225
>500	0.005776318060720052	2.2189584902741157
>1k	0.0025993431273240238	3.66320879474264
>5k	0.0	0.0
>10k+	2.8881590303600263E-4	3.0780423374524615
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20283	2.842109688086764	No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCTGCCCAGATCGTATG	4680	0.6557744584255808	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4451	0.6236863492419359	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC	2946	0.41280161421405154	No Hit
GAATGATACCTGTCCCTTATACACATCTGACGCTGCCCAGATCGTATGCC	2692	0.3772104363422358	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT	2415	0.3383964352773029	No Hit
GAATCTTTCTCTTACCCACATCTGACGCTGCCCAGATCGTATGCCGTCTT	2111	0.29579912002914555	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATG	1964	0.275201076142701	No Hit
GAACTGTCCCTTATCCACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	1802	0.252501191043354	No Hit
CGTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1078	0.15105232183392653	No Hit
GAACTGTCCCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	987	0.13830115180898467	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCCAGA	953	0.13353697839307233	No Hit
GAATGACTCTCTCTCCTACACATCTGACGCTGCCCAGATCGTATGCCGTC	935	0.13101476893758932	No Hit
GAATCTGTCTCTTACCCACATCTGACGCTGCCCAGATCGTATGCCGTCTT	918	0.12863268222963317	No Hit
GCTGTCTCCTATACCCATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	911	0.12765182299694533	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCCT	907	0.12709133200683798	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	900	0.12611047277415016	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	846	0.11854384440770115	No Hit
GAATGATACGGCGACCGTCTCTTATACACATCTGACGCTGCCCAGATCGT	740	0.10369083316985679	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03797326457977188	0.0
2	0.0	0.0	0.0	0.252501191043354	0.0
3	0.0	0.0	0.0	0.531065213126699	0.0
4	0.0	0.0	0.0	0.6686657511980495	0.0
5	0.0	0.0	0.0	1.697446963540061	0.0
6	0.0	0.0	0.0	1.8332259058935627	0.0
7	0.0	0.0	0.0	1.9215032368354679	0.0
8	1.401227475268335E-4	0.0	0.0	2.0607852478771402	0.0
9	1.401227475268335E-4	0.0	0.0	2.1775074965669927	0.0
10	1.401227475268335E-4	0.0	0.0	3.0316957654905696	0.0
11	1.401227475268335E-4	0.0	0.0	3.1552840288092368	0.0
12	1.401227475268335E-4	0.0	0.0	3.8060140683238517	0.0
13	1.401227475268335E-4	0.0	0.0	3.8809797382507076	0.0
14	1.401227475268335E-4	0.0	0.0	3.918532634587899	0.0
15	1.401227475268335E-4	0.0	0.0	4.00078468738615	0.0
16	1.401227475268335E-4	0.0	0.0	4.116806322338369	0.0
17	1.401227475268335E-4	0.0	0.0	4.175237508057058	0.0
18	1.401227475268335E-4	0.0	0.0	4.243757531597679	0.0
19	1.401227475268335E-4	0.0	0.0	4.495698231650926	0.0
20	1.401227475268335E-4	0.0	0.0	4.555951013087465	0.0
21	1.401227475268335E-4	0.0	0.0	4.593083541182075	0.0
22	1.401227475268335E-4	0.0	0.0	4.6702911750693605	0.0
23	1.401227475268335E-4	0.0	0.0	4.721576100664182	0.0
24	1.401227475268335E-4	0.0	0.0	4.779446795392764	0.0
25	1.401227475268335E-4	0.0	0.0	4.823725583611243	0.0
26	1.401227475268335E-4	0.0	0.0	4.894767816607348	0.0
27	1.401227475268335E-4	0.0	0.0	4.981784042821512	0.0
28	1.401227475268335E-4	0.0	0.0	5.035451055124289	0.0
29	1.401227475268335E-4	0.0	0.0	5.093181627105344	0.0
30	1.401227475268335E-4	0.0	0.0	5.1528739175517755	0.0
31	1.401227475268335E-4	0.0	0.0	5.206120561611972	0.0
32	1.401227475268335E-4	0.0	0.0	5.263991256340554	0.0
33	1.401227475268335E-4	0.0	0.0	5.326626124485049	0.0
34	1.401227475268335E-4	0.0	0.0	5.390522097357285	0.0
35	1.401227475268335E-4	0.0	0.0	5.461564330353389	0.0
36	1.401227475268335E-4	0.0	0.0	5.517333183869069	0.0
37	1.401227475268335E-4	0.0	0.0	5.57352240562733	0.0
38	1.401227475268335E-4	0.0	0.0	5.63853936047978	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	25	4.4411376E-5	44.0	1
CGGGTAT	20	7.8544737E-4	44.0	6
CGAACGC	25	4.4411376E-5	44.0	24
ATAGACG	20	7.8544737E-4	44.0	1
TAACGTA	20	7.8544737E-4	44.0	24
CGGTCTA	75	0.0	44.0	31
ACGTAGT	20	7.8544737E-4	44.0	35
GCGTAGC	20	7.8544737E-4	44.0	39
CGACGGT	85	0.0	43.999996	28
CGTTTTT	17785	0.0	43.492832	1
TACGGCT	1110	0.0	42.414413	7
GCGGAAT	135	0.0	42.370373	8
CCTTATC	365	0.0	42.19178	9
ACGGCTG	1135	0.0	41.48018	8
TGATCCG	250	0.0	41.36	4
ACCCGTC	155	0.0	41.16129	17
TGTACGG	75	0.0	41.066666	2
CGACCGT	130	0.0	40.615387	12
GATACCT	1200	0.0	40.333332	5
CGCAAGA	290	0.0	40.206894	20
>>END_MODULE