FastQCFastQC Report
Thu 26 May 2016
SRR1546123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546123_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1191719
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334782.809219287432692No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT46050.38641659652988664No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT33330.27968002524084956No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT30980.2599606115199976No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA18640.15641271138582163No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT14380.12066602949185169No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC14050.11789692033105119No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT13100.10992524244389827No Hit
GGGCAGGGGCTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT12140.1018696521579332No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA207.856598E-444.00000433
CTATGCG207.856598E-444.0000041
GACGTAG207.856598E-444.0000041
TAGCGAT207.856598E-444.00000428
CGTCTCA207.856598E-444.00000418
CGTCTAA207.856598E-444.00000426
CTAAGCG207.856598E-444.00000414
ACAATCG207.856598E-444.0000042
ACGTCTA207.856598E-444.00000425
GTCGGAC207.856598E-444.00000427
AAACTCG351.4461511E-744.01
CGTTCGA254.442939E-544.014
CCGTAAT454.802132E-1044.035
TACGCAC254.442939E-544.020
TCTACGG302.527795E-644.02
GGGCGTA254.442939E-544.07
TACTGGC254.442939E-544.042
TATTACG254.442939E-544.01
GTATAGA454.802132E-1044.09
CGTCGGA254.442939E-544.026
Produced by FastQC (version 0.11.5)