FastQCFastQC Report
Thu 26 May 2016
SRR1546122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546122_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1041613
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171661.6480209060370792No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT27820.2670857602583685No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT19310.18538555106359078No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT18210.17482500698435985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTA408.310963E-944.039
GTACATT207.8561413E-444.020
GTCGACG254.4425517E-544.01
CCTACCG254.4425517E-544.01
ATTAACG302.5274876E-644.01
TAACGAG207.8561413E-444.01
TCGATAA254.4425517E-544.038
CGTTAGG1500.044.02
CTAACGA502.7284841E-1144.024
CCACTCG207.8561413E-444.037
CGTAGTG207.8561413E-444.01
CACTAGG254.4425517E-544.02
GTACTAG502.7284841E-1144.01
TACTCGT207.8561413E-444.016
CTATTAG207.8561413E-444.01
CGGTCTA2200.044.031
GCTACGA351.4459147E-744.010
ATACGTA207.8561413E-444.026
CGCGTAC207.8561413E-444.034
CACCGAA207.8561413E-444.036
Produced by FastQC (version 0.11.5)