##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546122_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1041613
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.193543091340064	28.0	25.0	31.0	16.0	33.0
2	26.363880827140214	30.0	25.0	31.0	16.0	33.0
3	26.652372810247183	30.0	25.0	31.0	16.0	33.0
4	27.41905006945958	32.0	19.0	35.0	10.0	35.0
5	31.409543659689348	33.0	32.0	35.0	19.0	37.0
6	30.080545269692294	33.0	28.0	35.0	17.0	36.0
7	30.246181643278263	32.0	28.0	35.0	18.0	35.0
8	32.54710530686541	35.0	32.0	35.0	28.0	35.0
9	27.44546391030066	31.0	23.0	34.0	10.0	37.0
10	32.00677122885371	35.0	31.0	37.0	18.0	39.0
11	33.964609696691575	35.0	32.0	39.0	27.0	39.0
12	33.476213334510994	35.0	32.0	39.0	25.0	39.0
13	33.238494527238046	35.0	31.0	39.0	22.0	39.0
14	33.103252359561566	35.0	31.0	39.0	21.0	40.0
15	32.09324000372499	34.0	28.0	38.0	18.0	40.0
16	30.848416830435102	34.0	27.0	38.0	17.0	40.0
17	30.6691631152837	32.0	26.0	38.0	17.0	40.0
18	31.79229713914861	34.0	29.0	38.0	18.0	39.0
19	31.472216648601737	34.0	29.0	37.0	18.0	39.0
20	31.500408501045975	34.0	29.0	36.0	18.0	39.0
21	32.51471707822387	34.0	31.0	36.0	23.0	39.0
22	32.76765458956445	34.0	31.0	37.0	25.0	39.0
23	34.0356946389878	35.0	33.0	38.0	28.0	39.0
24	33.66668618767239	35.0	33.0	38.0	26.0	40.0
25	32.63552490224296	35.0	31.0	37.0	23.0	39.0
26	26.040606252034106	33.0	18.0	35.0	0.0	38.0
27	29.298521619833853	33.0	23.0	36.0	11.0	39.0
28	30.851057926504375	33.0	27.0	36.0	18.0	39.0
29	32.09220027015792	34.0	30.0	37.0	23.0	39.0
30	31.909265725370172	34.0	30.0	37.0	21.0	39.0
31	30.77657152896517	33.0	29.0	36.0	18.0	38.0
32	30.818181032686805	34.0	29.0	36.0	18.0	39.0
33	30.2721874630981	33.0	28.0	36.0	15.0	38.0
34	29.972847881122835	34.0	27.0	37.0	10.0	39.0
35	28.88780477970225	33.0	24.0	36.0	8.0	38.0
36	28.573961730508355	33.0	23.0	36.0	8.0	38.0
37	28.708170884963994	33.0	23.0	36.0	8.0	39.0
38	28.451925043178225	33.0	23.0	36.0	8.0	38.0
39	28.526036061377884	33.0	23.0	36.0	7.0	39.0
40	28.372315821711133	33.0	23.0	36.0	7.0	38.0
41	27.78842045942207	32.0	22.0	35.0	7.0	38.0
42	27.951132522347553	33.0	22.0	36.0	7.0	38.0
43	27.592907346586497	32.0	22.0	35.0	7.0	38.0
44	27.235580777121637	32.0	21.0	35.0	7.0	38.0
45	26.522088337991175	31.0	20.0	34.0	7.0	37.0
46	26.05353619818493	30.0	18.0	34.0	7.0	37.0
47	26.155316801921636	30.0	18.0	34.0	7.0	37.0
48	26.270735868311935	31.0	19.0	34.0	7.0	37.0
49	26.138871154641887	31.0	18.0	34.0	7.0	37.0
50	27.811853346684423	33.0	21.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	6.0
11	12.0
12	34.0
13	123.0
14	346.0
15	855.0
16	1862.0
17	3281.0
18	5778.0
19	9221.0
20	13519.0
21	19074.0
22	25667.0
23	34111.0
24	43690.0
25	52783.0
26	59698.0
27	62210.0
28	64758.0
29	73333.0
30	85823.0
31	98544.0
32	105263.0
33	100888.0
34	83681.0
35	61833.0
36	29464.0
37	5614.0
38	139.0
39	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.18758214423207	20.757613432244028	24.397544961516417	30.65725946200748
2	45.16379883891618	16.60674357942921	26.045469862607323	12.183987719047286
3	27.203193508529562	13.67734465679672	46.244046493275334	12.875415341398389
4	25.347993928647206	21.737343907958138	40.1878624786749	12.726799684719756
5	16.59810313427348	26.230471393886212	42.952996938402265	14.218428533438043
6	16.56584547235874	33.43881076753074	40.62612505796299	9.369218702147535
7	70.68344961132398	3.5102288469901968	24.311140509959074	1.4951810317267544
8	72.63542217695056	2.3739143040649453	23.163209368546667	1.8274541504378305
9	61.98885766594695	2.9575283718617182	22.533512926585978	12.520101035605355
10	41.74208655229917	21.209988738619813	28.629538993848964	8.418385715232048
11	37.59006464013026	16.721085470323434	33.43429853506053	12.254551354485784
12	33.17326108641117	17.118545947487217	39.413294572936394	10.294898393165216
13	16.598391149112	33.66951065318885	39.07574118218571	10.65635701551344
14	11.587316978570735	37.39747871810356	39.95716259301679	11.058041710308915
15	9.94870455725879	20.142701751994263	55.64782697604581	14.260766714701143
16	10.986614030354843	12.989469217454083	56.7603322923197	19.26358445987137
17	11.363145429252516	14.474473724886305	41.12141457527892	33.04096627058226
18	15.93883716889094	21.25184689515204	45.98051291602543	16.828803019931588
19	22.705745800023617	22.32527819833278	38.568547051544094	16.40042895009951
20	24.623252589973436	18.92324692568161	37.69547807103022	18.758022413314734
21	20.318198793601848	22.12126768771127	41.087524829279204	16.473008689407678
22	17.093392651589408	19.796603921034013	37.244062814116184	25.865940613260396
23	14.362723967538807	26.6671978940355	37.8168283229952	21.15324981543049
24	15.094185652444814	21.811267716512756	49.73449832135351	13.360048309688915
25	17.08369615202575	18.343953080462704	46.82007617032429	17.75227459718725
26	10.260144602649929	39.645146517948604	32.20524321413039	17.889465665271075
27	12.755793178464556	28.603905673220286	43.410844526710015	15.229456621605145
28	11.316775040250073	24.569489820115535	50.76194325531651	13.35179188431788
29	12.608425586086195	22.485318443606214	47.36058401728857	17.54567195301902
30	14.787929874147116	24.335621771233654	46.25911926982479	14.617329084794447
31	26.65606132027922	22.092754218697348	36.43224498926185	14.818939471761587
32	24.26198597751756	23.740199095057378	37.71189491682612	14.285920010598947
33	22.838904660368105	21.827876572201	34.93303174979575	20.400187017635147
34	15.677991730133936	23.800010176524296	35.47968391331521	25.042314180026555
35	17.013516536371952	21.465841920175727	37.646515548481055	23.87412599497126
36	28.940018989778356	21.169474651334035	32.18201001715608	17.708496341731525
37	16.849924108090047	28.446745576332095	33.88561778702839	20.81771252854947
38	18.220586724628053	31.52744829413611	29.531601468107638	20.720363513128195
39	17.167796484874902	26.55160793884101	32.2567018652801	24.02389371100399
40	19.912865910851725	21.436560411592406	35.67188581555722	22.97868786199865
41	15.497022406594388	20.888372168934144	36.3061904949343	27.308414929537168
42	19.327139734239108	24.183069911761855	28.926962317098575	27.56282803690046
43	18.996786714451527	22.36080002841746	30.691149208007197	27.951264049123807
44	16.604823480505715	25.610183436650658	34.26368526506486	23.521307817778773
45	18.360849950989476	32.63140917020045	27.70558739186243	21.30215348694765
46	21.875783040342238	27.573100566141168	29.88461165519248	20.666504738324118
47	21.390862057213187	24.088313029887303	29.66850452135294	24.852320391546574
48	18.5842534607383	25.236916205922928	33.45513160838046	22.72369872495831
49	19.17045966208179	23.381908635932923	34.23315569218126	23.214476009804024
50	17.702160015283987	27.50589710381879	33.39455248734415	21.397390393553074
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	532.0
1	823.5
2	1115.0
3	14361.0
4	27607.0
5	21955.5
6	16304.0
7	16407.0
8	16510.0
9	18082.5
10	19655.0
11	20878.5
12	22102.0
13	22116.5
14	22131.0
15	21623.0
16	21115.0
17	20069.5
18	19024.0
19	17968.0
20	16912.0
21	15778.5
22	14645.0
23	13317.0
24	11989.0
25	10870.5
26	9752.0
27	10098.0
28	10444.0
29	10931.5
30	11419.0
31	12561.5
32	13704.0
33	14828.5
34	15953.0
35	17247.5
36	18542.0
37	21096.0
38	23650.0
39	29971.5
40	36293.0
41	52010.5
42	67728.0
43	78198.5
44	88669.0
45	93907.0
46	99145.0
47	97118.0
48	95091.0
49	92706.5
50	90322.0
51	78689.5
52	67057.0
53	56653.5
54	46250.0
55	40251.0
56	34252.0
57	31420.5
58	28589.0
59	25435.5
60	22282.0
61	19604.5
62	16927.0
63	14704.5
64	12482.0
65	10131.0
66	7780.0
67	6502.0
68	5224.0
69	4454.5
70	3685.0
71	3283.5
72	2882.0
73	2352.0
74	1822.0
75	1362.5
76	903.0
77	740.5
78	578.0
79	400.5
80	223.0
81	177.0
82	131.0
83	99.5
84	68.0
85	61.5
86	55.0
87	47.5
88	40.0
89	28.5
90	17.0
91	10.5
92	4.0
93	4.0
94	4.0
95	3.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1041613.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.59510505917895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.75369930494132	36.10092726048038
2	6.470626627425251	5.512340419877987
3	2.3791878074980057	3.040252638476856
4	1.2614632349139927	2.1492863607781305
5	0.7724698639865973	1.64517175057794
6	0.5655324209697699	1.44533477313475
7	0.3979701146365567	1.1866105190350338
8	0.3450991807316937	1.1759628687282462
9	0.2418652866163972	0.9272049564234481
>10	2.208268653659502	20.052703577517875
>50	0.43711982616817446	13.084213909015938
>100	0.16249936655122013	10.292892231662327
>500	0.0032104738070457078	0.8940324899139688
>1k	7.408785708567018E-4	0.6873288962346008
>5k	0.0	0.0
>10k+	2.4695952361890056E-4	1.8057373481425092
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17166	1.6480209060370792	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2782	0.2670857602583685	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1931	0.18538555106359078	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1821	0.17482500698435985	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04253019115544833	0.0
2	0.0	0.0	0.0	0.1864416054715139	0.0
3	0.0	0.0	0.0	0.2584453151026341	0.0
4	0.0	0.0	0.0	0.38459581437635665	0.0
5	0.0	0.0	0.0	0.6822111475183201	0.0
6	0.0	0.0	0.0	0.8833415097545826	0.0
7	0.0	0.0	0.0	1.0077639199971582	0.0
8	0.0	0.0	0.0	1.306819327331744	0.0
9	0.0	0.0	0.0	1.442858336061474	0.0
10	0.0	0.0	0.0	1.7206006453452483	0.0
11	0.0	0.0	0.0	1.9668533322836792	0.0
12	0.0	0.0	0.0	2.2072497175054457	0.0
13	0.0	0.0	0.0	2.3217836182920144	0.0
14	0.0	0.0	0.0	2.3612416511698684	0.0
15	0.0	0.0	0.0	2.4082840747955334	0.0
16	0.0	0.0	0.0	2.5047690457012344	0.0
17	0.0	0.0	0.0	2.6486804600173	0.0
18	0.0	0.0	0.0	2.8521149409617585	0.0
19	0.0	0.0	0.0	2.9221025467232074	0.0
20	0.0	0.0	0.0	2.994298266246677	0.0
21	0.0	0.0	0.0	3.0833908562969166	0.0
22	0.0	0.0	0.0	3.170947367208359	0.0
23	0.0	0.0	0.0	3.2747287140233463	0.0
24	0.0	0.0	0.0	3.3523967154787813	0.0
25	0.0	0.0	0.0	3.406927524906083	0.0
26	0.0	0.0	0.0	3.468754710242672	0.0
27	0.0	0.0	0.0	3.5443106028822604	0.0
28	0.0	0.0	0.0	3.63897147981064	0.0
29	0.0	0.0	0.0	3.7362244902857396	0.0
30	0.0	0.0	0.0	3.847398217956189	0.0
31	0.0	0.0	0.0	3.9498354955247295	0.0
32	0.0	0.0	0.0	4.040848184498465	0.0
33	0.0	0.0	0.0	4.150389828083943	0.0
34	0.0	0.0	0.0	4.2530191155448325	0.0
35	0.0	0.0	0.0	4.416899558665262	0.0
36	0.0	0.0	0.0	4.5292253456898095	0.0
37	0.0	0.0	0.0	4.628974484765455	0.0
38	0.0	0.0	0.0	4.734387915665415	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGTA	40	8.310963E-9	44.0	39
GTACATT	20	7.8561413E-4	44.0	20
GTCGACG	25	4.4425517E-5	44.0	1
CCTACCG	25	4.4425517E-5	44.0	1
ATTAACG	30	2.5274876E-6	44.0	1
TAACGAG	20	7.8561413E-4	44.0	1
TCGATAA	25	4.4425517E-5	44.0	38
CGTTAGG	150	0.0	44.0	2
CTAACGA	50	2.7284841E-11	44.0	24
CCACTCG	20	7.8561413E-4	44.0	37
CGTAGTG	20	7.8561413E-4	44.0	1
CACTAGG	25	4.4425517E-5	44.0	2
GTACTAG	50	2.7284841E-11	44.0	1
TACTCGT	20	7.8561413E-4	44.0	16
CTATTAG	20	7.8561413E-4	44.0	1
CGGTCTA	220	0.0	44.0	31
GCTACGA	35	1.4459147E-7	44.0	10
ATACGTA	20	7.8561413E-4	44.0	26
CGCGTAC	20	7.8561413E-4	44.0	34
CACCGAA	20	7.8561413E-4	44.0	36
>>END_MODULE