FastQCFastQC Report
Thu 26 May 2016
SRR1546121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546121_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences527641
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218454.140125577807638No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT9040.1713286116886292No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT8110.15370299123836093No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT6760.12811741316539085No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT5540.10499563149944754RNA PCR Primer, Index 34 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA302.525103E-644.00000414
TTAACGG302.525103E-644.0000042
ATTGGGC650.044.0000044
TATAGCG302.525103E-644.0000041
TTCGAAT302.525103E-644.00000416
GCGAGAC254.4395554E-544.021
AGCGTCG207.852605E-444.017
GCTATAT207.852605E-444.036
CGATTGA207.852605E-444.010
ATCGTAC254.4395554E-544.042
AGACCGA207.852605E-444.017
TCGTACT254.4395554E-544.043
TCGAGGT207.852605E-444.024
GGACCGT207.852605E-444.08
CGATAGA207.852605E-444.010
GTCGGCC207.852605E-444.016
CGTTTTT109850.043.479291
CTCACGA1400.042.42857424
CGACGGT1350.042.3703728
AGGGCGC2250.041.0666666
Produced by FastQC (version 0.11.5)