##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546121_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	527641
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.159746873347597	31.0	28.0	33.0	25.0	33.0
2	28.980812711673277	31.0	28.0	33.0	16.0	33.0
3	28.414334746541684	31.0	28.0	33.0	16.0	34.0
4	31.854450279640893	35.0	32.0	35.0	25.0	37.0
5	33.66630151940429	35.0	33.0	35.0	28.0	37.0
6	33.570634200147445	35.0	33.0	37.0	28.0	37.0
7	33.97100680197331	35.0	33.0	37.0	30.0	37.0
8	34.52039928663618	35.0	35.0	37.0	32.0	37.0
9	36.40414789601263	39.0	37.0	39.0	32.0	39.0
10	36.149658574674824	38.0	35.0	39.0	31.0	39.0
11	36.43982935367039	39.0	35.0	39.0	32.0	39.0
12	35.50412496375377	37.0	35.0	39.0	30.0	39.0
13	36.06352804274118	38.0	35.0	39.0	31.0	39.0
14	36.824230869094706	39.0	35.0	40.0	31.0	41.0
15	37.21008791962717	39.0	35.0	41.0	32.0	41.0
16	36.79944318201201	38.0	35.0	41.0	31.0	41.0
17	36.31879630278921	38.0	34.0	40.0	30.0	41.0
18	35.88347948699968	38.0	34.0	39.0	29.0	40.0
19	35.2949524392532	37.0	34.0	39.0	29.0	40.0
20	35.401477519753016	35.0	34.0	39.0	30.0	40.0
21	35.62446246595697	36.0	34.0	39.0	30.0	40.0
22	35.9030154972794	36.0	35.0	40.0	31.0	41.0
23	36.205219078881285	37.0	35.0	40.0	32.0	41.0
24	35.875142379003904	36.0	35.0	40.0	31.0	41.0
25	35.536902932107246	36.0	35.0	40.0	30.0	41.0
26	35.58651621083274	36.0	34.0	40.0	30.0	41.0
27	35.9172846689321	37.0	35.0	40.0	31.0	41.0
28	35.78055723493815	36.0	35.0	40.0	31.0	41.0
29	35.817347400979074	37.0	35.0	40.0	31.0	41.0
30	35.40166514732555	36.0	35.0	40.0	30.0	41.0
31	34.732073512103874	36.0	34.0	40.0	27.0	41.0
32	34.564078606476755	36.0	34.0	40.0	24.0	41.0
33	33.96147001465011	36.0	33.0	40.0	21.0	41.0
34	33.27117490869739	36.0	33.0	40.0	16.0	41.0
35	32.74328189052784	36.0	32.0	40.0	12.0	41.0
36	32.22952727327861	36.0	31.0	40.0	10.0	41.0
37	32.07102745995857	36.0	31.0	40.0	10.0	41.0
38	31.872532650040462	36.0	31.0	40.0	10.0	41.0
39	31.627204860880788	35.0	30.0	40.0	8.0	40.0
40	31.43810659141348	35.0	30.0	40.0	8.0	40.0
41	31.102999956409757	35.0	29.0	39.0	8.0	40.0
42	31.274675394823376	35.0	29.0	40.0	8.0	40.0
43	31.055888378651392	35.0	29.0	39.0	8.0	40.0
44	30.931796429769484	35.0	28.0	39.0	8.0	40.0
45	30.883932446492974	35.0	28.0	39.0	8.0	40.0
46	30.639205444611015	35.0	27.0	39.0	7.0	40.0
47	30.568416783381124	35.0	27.0	39.0	7.0	40.0
48	30.535060770485995	35.0	27.0	39.0	7.0	40.0
49	30.424273322202026	35.0	27.0	38.0	7.0	40.0
50	30.59057578922032	35.0	27.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	9.0
14	22.0
15	64.0
16	158.0
17	386.0
18	793.0
19	1419.0
20	2085.0
21	3165.0
22	4303.0
23	6214.0
24	9112.0
25	14032.0
26	20042.0
27	22615.0
28	19872.0
29	17977.0
30	18113.0
31	19520.0
32	24046.0
33	32879.0
34	48401.0
35	48056.0
36	50926.0
37	70041.0
38	77089.0
39	16295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.732914993338273	20.764497072820344	24.146152402864825	30.356435530976555
2	45.82888744430399	18.9232830655692	24.18443600857401	11.063393481552799
3	25.25486078602686	16.889134847367814	46.2644108399461	11.591593526659224
4	22.42035778114286	21.97516872267318	42.759944735151365	12.844528761032596
5	17.67641256081313	25.338440341065233	43.496240815251284	13.48890628287036
6	15.167888772858817	33.69052063808537	42.08069501801414	9.060895571041675
7	66.64777756087946	4.746977585138381	25.966708424857053	2.638536429125106
8	66.93471508089782	4.096535333683319	25.818122549233287	3.150627036185588
9	63.40485292083065	5.573676041096124	26.74394142987372	4.277529608199514
10	29.930009229760383	26.257815446487285	32.1275261020277	11.68464922172462
11	26.15793693060244	20.289742457466346	39.50166874825876	14.050651863672458
12	24.638532638669094	17.895690441038507	43.312024653125896	14.153752267166503
13	19.073385123597294	23.664954012292448	42.82172916812757	14.439931695982686
14	14.831675324699939	28.369668012910292	42.31570329068439	14.482953371705385
15	14.70355791153455	22.12849266831046	48.136517063685346	15.031432356469645
16	16.725576670501344	18.28004268053468	48.30538187896695	16.688998769997024
17	16.29062184326085	18.329129085874676	42.27343970616385	23.10680936470062
18	17.81969179802176	21.739212836000235	43.802320138124216	16.638775227853785
19	20.478317644004164	23.32532915372384	39.98059286522465	16.21576033704735
20	21.457392431596485	21.089718198547878	40.24592478598138	17.206964583874264
21	19.85706190383234	22.498630697766096	41.974372726910914	15.669934671490655
22	18.141311990539023	19.866538043859368	40.741905954995914	21.250244010605694
23	16.300666551689503	23.597483895300027	40.85012347410456	19.25172607890592
24	16.777316395048906	22.367670442592598	45.49191590494294	15.363097257415554
25	18.967441878095144	21.253655421015424	42.21923618520926	17.55966651568017
26	15.61004546651985	23.70608046000974	41.15752945658128	19.526344616889133
27	16.075134419046282	23.02285076406117	42.89659067434108	18.00542414255147
28	15.589766526862014	22.605521557270947	45.334801503294855	16.469910412572183
29	16.91737374464835	22.089640494199656	43.605405948362616	17.387579812789376
30	18.151356698967668	22.053441639296416	44.09058431774635	15.704617343989568
31	22.86516779401146	22.326354472074765	39.21188838623231	15.596589347681473
32	22.043965499269387	22.50355829058015	38.637823823395074	16.814652386755387
33	20.693046977016568	21.669657968201864	38.08915531583027	19.548139738951296
34	17.709200005306638	23.711008052823797	38.04139557009406	20.538396371775505
35	18.53059182284925	23.59956864610597	37.48249283130007	20.38734669974471
36	23.499500607420575	24.06598425823619	33.7098519637405	18.72466317060274
37	18.49439296794601	27.023298037870447	34.15617815901342	20.326130835170126
38	19.77859946440857	28.042930704778442	32.65989564874602	19.51857418206697
39	19.587560481463722	24.816115502775563	32.84164801446438	22.754676001296335
40	20.197444853603113	23.569434520820025	35.40153248136517	20.83158814421169
41	18.22375440877415	23.78966001504811	34.77136916956795	23.215216406609798
42	19.683648541337764	25.480203395869538	31.941983280298537	22.89416478249416
43	19.398795772125364	24.163209454913474	32.957635968395174	23.480358804565984
44	19.005156915402708	24.92167970267663	34.27690418295773	21.79625919896293
45	19.875824661085854	26.852158948982357	31.89005403295043	21.381962356981358
46	20.93336188810195	25.42922176252414	32.75029802460385	20.887118324770064
47	19.75794147914965	24.776315714662054	33.02302133458166	22.442721471606642
48	18.69850902412815	25.831578668071664	33.655079874384285	21.8148324334159
49	19.614472719140476	24.89704173860636	34.4694593483069	21.019026193946264
50	18.860930064191372	24.59247859813775	35.13051487659223	21.41607646107865
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	440.0
1	586.5
2	733.0
3	15708.0
4	30683.0
5	20703.0
6	10723.0
7	10378.0
8	10033.0
9	10311.0
10	10589.0
11	10352.0
12	10115.0
13	9729.0
14	9343.0
15	8882.5
16	8422.0
17	7740.0
18	7058.0
19	6564.0
20	6070.0
21	5667.0
22	5264.0
23	5015.5
24	4767.0
25	4739.0
26	4711.0
27	5169.0
28	5627.0
29	5922.5
30	6218.0
31	7334.5
32	8451.0
33	9758.0
34	11065.0
35	11639.5
36	12214.0
37	14329.5
38	16445.0
39	18730.5
40	21016.0
41	24728.0
42	28440.0
43	30595.0
44	32750.0
45	33693.5
46	34637.0
47	37149.0
48	39661.0
49	40949.0
50	42237.0
51	38522.0
52	34807.0
53	30540.0
54	26273.0
55	23446.0
56	20619.0
57	19643.0
58	18667.0
59	16930.0
60	15193.0
61	12906.0
62	10619.0
63	9385.5
64	8152.0
65	6561.5
66	4971.0
67	4308.0
68	3645.0
69	3080.5
70	2516.0
71	2226.5
72	1937.0
73	1577.0
74	1217.0
75	911.0
76	605.0
77	464.0
78	323.0
79	241.0
80	159.0
81	136.5
82	114.0
83	77.0
84	40.0
85	45.5
86	51.0
87	26.5
88	2.0
89	6.0
90	10.0
91	5.5
92	1.0
93	2.5
94	4.0
95	3.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	527641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.95782090073168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.20410032086875	30.466613340032573
2	7.861209915031125	6.125112158656774
3	3.6395503006004923	4.253668463099944
4	2.1856630654837272	3.4059468101783694
5	1.4715528552879507	2.8664246291134154
6	1.071617933045907	2.504873970576883
7	0.7735741831679244	2.1095735136900062
8	0.5745476983324157	1.7906501064449492
9	0.46658994328041115	1.635959469996073
>10	3.3044951417966484	26.28902497288006
>50	0.3910284752126361	10.324099767419465
>100	0.05363233450544405	3.5193074989543485
>500	0.0019502667092888748	0.5593890643577588
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.875666773222187E-4	4.149356234599403
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21845	4.140125577807638	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	904	0.1713286116886292	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	811	0.15370299123836093	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	676	0.12811741316539085	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT	554	0.10499563149944754	RNA PCR Primer, Index 34 (95% over 21bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.057046362962696225	0.0
2	0.0	0.0	0.0	0.23121781665943322	0.0
3	0.0	0.0	0.0	0.3015307756599658	0.0
4	0.0	0.0	0.0	0.43476530443995065	0.0
5	0.0	0.0	0.0	0.851525942828552	0.0
6	0.0	0.0	0.0	1.1219749791998725	0.0
7	0.0	0.0	0.0	1.280226517651206	0.0
8	0.0	0.0	0.0	1.618335193815492	0.0
9	0.0	0.0	0.0	1.7466421297814234	0.0
10	0.0	0.0	0.0	2.0875936479538173	0.0
11	1.8952280054051904E-4	0.0	0.0	2.390261560417026	0.0
12	1.8952280054051904E-4	0.0	0.0	2.705058932114828	0.0
13	1.8952280054051904E-4	0.0	0.0	2.8578143093504864	0.0
14	1.8952280054051904E-4	0.0	0.0	2.928127268351019	0.0
15	1.8952280054051904E-4	0.0	0.0	3.006589707774794	0.0
16	1.8952280054051904E-4	0.0	0.0	3.1563127202018038	0.0
17	3.790456010810381E-4	0.0	0.0	3.3312422651007028	0.0
18	3.790456010810381E-4	0.0	0.0	3.6005541646687806	0.0
19	3.790456010810381E-4	0.0	0.0	3.711425002984984	0.0
20	3.790456010810381E-4	0.0	0.0	3.831582458527673	0.0
21	3.790456010810381E-4	0.0	0.0	3.9752407413373865	0.0
22	3.790456010810381E-4	0.0	0.0	4.117572364543316	0.0
23	3.790456010810381E-4	0.0	0.0	4.294586660248161	0.0
24	3.790456010810381E-4	0.0	0.0	4.43672876065355	0.0
25	3.790456010810381E-4	0.0	0.0	4.527699704912999	0.0
26	3.790456010810381E-4	0.0	0.0	4.647857160455689	0.0
27	3.790456010810381E-4	0.0	0.0	4.761760363580541	0.0
28	3.790456010810381E-4	0.0	0.0	4.893478709956201	0.0
29	3.790456010810381E-4	0.0	0.0	5.023301828326457	0.0
30	3.790456010810381E-4	0.0	0.0	5.157104925508063	0.0
31	3.790456010810381E-4	0.0	0.0	5.2793471318566985	0.0
32	3.790456010810381E-4	0.0	0.0	5.414476888642088	0.0
33	3.790456010810381E-4	0.0	0.0	5.557187557449099	0.0
34	3.790456010810381E-4	0.0	0.0	5.71089054868746	0.0
35	3.790456010810381E-4	0.0	0.0	5.89719146161879	0.0
36	3.790456010810381E-4	0.0	0.0	6.03573262881391	0.0
37	3.790456010810381E-4	0.0	0.0	6.176169024014434	0.0
38	3.790456010810381E-4	0.0	0.0	6.333472948463065	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGA	30	2.525103E-6	44.000004	14
TTAACGG	30	2.525103E-6	44.000004	2
ATTGGGC	65	0.0	44.000004	4
TATAGCG	30	2.525103E-6	44.000004	1
TTCGAAT	30	2.525103E-6	44.000004	16
GCGAGAC	25	4.4395554E-5	44.0	21
AGCGTCG	20	7.852605E-4	44.0	17
GCTATAT	20	7.852605E-4	44.0	36
CGATTGA	20	7.852605E-4	44.0	10
ATCGTAC	25	4.4395554E-5	44.0	42
AGACCGA	20	7.852605E-4	44.0	17
TCGTACT	25	4.4395554E-5	44.0	43
TCGAGGT	20	7.852605E-4	44.0	24
GGACCGT	20	7.852605E-4	44.0	8
CGATAGA	20	7.852605E-4	44.0	10
GTCGGCC	20	7.852605E-4	44.0	16
CGTTTTT	10985	0.0	43.47929	1
CTCACGA	140	0.0	42.428574	24
CGACGGT	135	0.0	42.37037	28
AGGGCGC	225	0.0	41.066666	6
>>END_MODULE