FastQCFastQC Report
Thu 26 May 2016
SRR1546120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546120_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences529991
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222494.197995815023274No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT8890.16773869744957934No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT7560.14264393168940606No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT6750.12736065329411256No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT6200.11698311858125894RNA PCR Primer, Index 34 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC850.044.025
GATACGA207.852639E-444.011
TCCATCG207.852639E-444.02
CGACGGT800.044.028
ACCGCAG254.439582E-544.021
CGCATTC207.852639E-444.030
CGTTGAT254.439582E-544.025
TCGGGCA302.5251247E-644.05
TACGGGT302.5251247E-644.04
TAACGGT254.439582E-544.018
GCGCACC207.852639E-444.041
CGTTAGG950.044.02
CGCTCAT207.852639E-444.012
CTTATCG254.439582E-544.040
CCTCACG207.852639E-444.021
AACGCTC207.852639E-444.010
GGAACGC254.439582E-544.08
AATCTCG254.439582E-544.018
CGAGATA1000.044.019
CGGTCTA800.044.031
Produced by FastQC (version 0.11.5)