##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546120_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 529991 Sequences flagged as poor quality 0 Sequence length 50 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.112622667177366 31.0 28.0 33.0 25.0 33.0 2 28.9374498812244 31.0 28.0 33.0 16.0 33.0 3 28.40405969157967 31.0 28.0 33.0 16.0 34.0 4 31.80984582757066 33.0 30.0 35.0 25.0 37.0 5 33.68303424020408 35.0 33.0 35.0 30.0 37.0 6 33.64169580238155 35.0 33.0 37.0 28.0 37.0 7 34.02211735670983 35.0 33.0 37.0 30.0 37.0 8 34.55448300065473 35.0 35.0 37.0 32.0 37.0 9 36.5021934334734 39.0 37.0 39.0 32.0 39.0 10 36.12221716972552 38.0 35.0 39.0 31.0 39.0 11 36.46296635225881 39.0 35.0 39.0 32.0 39.0 12 35.450039717655585 37.0 35.0 39.0 29.0 39.0 13 36.03906292748368 38.0 35.0 39.0 31.0 39.0 14 36.824161165000916 39.0 35.0 40.0 31.0 41.0 15 37.23755309052418 39.0 36.0 41.0 32.0 41.0 16 36.81541573347472 38.0 35.0 41.0 31.0 41.0 17 36.36207973342943 38.0 34.0 40.0 30.0 41.0 18 35.94661984826158 38.0 34.0 39.0 30.0 40.0 19 35.33905670096285 37.0 34.0 39.0 29.0 40.0 20 35.35815325165899 35.0 34.0 39.0 30.0 40.0 21 35.501187756018496 36.0 34.0 39.0 30.0 40.0 22 35.854154127145556 36.0 35.0 39.0 31.0 40.0 23 36.20694502359474 37.0 35.0 40.0 32.0 41.0 24 35.78345292655913 36.0 35.0 40.0 31.0 41.0 25 35.45385676360542 36.0 34.0 40.0 30.0 41.0 26 35.534726061385946 36.0 34.0 40.0 30.0 41.0 27 35.913215507433144 37.0 35.0 40.0 31.0 41.0 28 35.78164912234359 36.0 35.0 40.0 31.0 41.0 29 35.81109301856069 37.0 35.0 40.0 31.0 41.0 30 35.447186839021796 36.0 35.0 40.0 30.0 41.0 31 34.76204312903427 36.0 34.0 40.0 27.0 41.0 32 34.640993903670065 36.0 34.0 40.0 25.0 41.0 33 34.07279368894943 36.0 33.0 40.0 21.0 41.0 34 33.29224458528541 36.0 33.0 40.0 16.0 41.0 35 32.811543969614576 36.0 33.0 40.0 12.0 41.0 36 32.27971418382576 36.0 31.0 40.0 10.0 41.0 37 32.10165644322262 36.0 31.0 40.0 10.0 41.0 38 31.93314414773081 36.0 31.0 40.0 10.0 41.0 39 31.661816898777527 35.0 30.0 40.0 9.0 40.0 40 31.45002084941065 35.0 30.0 40.0 8.0 40.0 41 31.134464547511183 35.0 29.0 39.0 8.0 40.0 42 31.297833359434406 35.0 29.0 39.0 8.0 40.0 43 31.103647043062995 35.0 29.0 39.0 8.0 40.0 44 30.9548256479827 35.0 28.0 39.0 8.0 40.0 45 30.880194191976845 35.0 28.0 39.0 8.0 40.0 46 30.61768218705601 35.0 27.0 39.0 7.0 40.0 47 30.54893951029357 35.0 27.0 38.0 7.0 40.0 48 30.5223918896736 35.0 27.0 38.0 7.0 40.0 49 30.491232869992132 35.0 27.0 38.0 7.0 40.0 50 30.673301999467917 35.0 27.0 39.0 7.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 5.0 13 5.0 14 26.0 15 50.0 16 165.0 17 413.0 18 758.0 19 1254.0 20 2084.0 21 3013.0 22 4381.0 23 6112.0 24 8947.0 25 13786.0 26 20131.0 27 22433.0 28 20433.0 29 18382.0 30 17852.0 31 19624.0 32 24307.0 33 33075.0 34 48610.0 35 48467.0 36 51980.0 37 71948.0 38 77346.0 39 14403.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.89683787083177 20.60374610134889 24.059087795830493 30.440328231988843 2 45.83002352870143 18.839942565062426 24.200033585475982 11.130000320760164 3 25.35514754024125 16.81839880299854 46.25078539069531 11.575668266064895 4 22.316794058767037 22.011694538209138 42.85374657305501 12.81776482996881 5 17.6867154347904 25.294769156457374 43.5765890364176 13.441926372334626 6 15.223466058857603 33.6139670296288 42.22449060455744 8.938076306956155 7 66.4826383844254 4.721023564551096 26.11591517591808 2.6804228751054264 8 66.81170057604751 4.132334322658309 25.837797245613604 3.2181678556805684 9 63.35635888156591 5.610661313116638 26.83875763928067 4.1942221660367816 10 29.886356560771787 25.912515495546153 32.27205745003217 11.929070493649892 11 26.127236122877555 20.22072072921993 39.67086233539815 13.981180812504363 12 24.648343085071257 17.648035532678858 43.297716376315826 14.405905005934065 13 19.041644103390436 23.642476947721754 42.91657782868011 14.399301120207703 14 14.776099971508948 28.438973492002695 42.3507191631556 14.434207373332756 15 14.742325813079846 22.114337790641727 48.15572339907659 14.987612997201838 16 16.785568056816057 18.15559132136206 48.4042181848371 16.654622436984777 17 16.30688068287952 18.36502884011238 42.3622287925644 22.9658616844437 18 17.80690615501018 21.651499742448458 43.83357453239772 16.708019570143644 19 20.543745082463666 23.001145302467403 40.1297380521556 16.325371562913332 20 21.53262979937395 20.82790085114653 40.230871844993594 17.408597504485925 21 19.85071444609437 22.386606565017143 42.1092056280201 15.653473360868391 22 18.112949087814698 19.823921538290275 40.862014637984416 21.201114735910608 23 16.329145211899824 23.460964431471478 40.91031734501152 19.299573011617177 24 16.875946950042547 22.25641567498316 45.52379191344759 15.343845461526707 25 18.9754165636775 21.230360515555923 42.25166087726018 17.5425620435064 26 15.650831806577848 23.664930159191382 41.21428477087347 19.469953263357304 27 16.005932176206766 22.967748508936943 42.964125805909916 18.06219350894638 28 15.61045376242238 22.587176008649205 45.36680811560951 16.435562113318905 29 16.912551345211522 22.08999775467885 43.722817934644176 17.27463296546545 30 18.16257257198707 22.05999724523624 44.19339196325976 15.584038219516936 31 22.775103728176514 22.215282901030395 39.3899141683538 15.619699202439286 32 21.953014296469185 22.487929040304458 38.80839485953535 16.750661803691006 33 20.66299239043682 21.77640752390135 38.09668466068292 19.463915424978914 34 17.78445294354055 23.73398793564419 38.08008060514235 20.401478515672906 35 18.503521757916644 23.556060385931083 37.567807755226035 20.37261010092624 36 23.485870514782327 23.943802819293158 33.87434880969677 18.695977856227746 37 18.3544626229502 26.96970325911195 34.34058314197788 20.33525097595997 38 19.86637508938831 27.911228681241756 32.720744314526094 19.501651914843837 39 19.56693604231015 24.689287176574695 32.91055885854666 22.833217922568497 40 20.398270914034388 23.516437071572913 35.367770396101065 20.71752161829163 41 18.344273770686673 23.699270364968463 34.675305807079745 23.281150057265123 42 19.683541795992763 25.57779283044429 31.961863503342506 22.776801870220435 43 19.505236881381006 24.16041027111781 33.005843495455586 23.3285093520456 44 19.035040217664072 24.94438584806157 34.32058280234948 21.699991131924882 45 19.855997554675454 26.72686894683117 32.11922466607924 21.297908832414137 46 20.95846910607916 25.4666588677921 32.742442796198425 20.83242922993032 47 19.82505363298622 24.7238160648011 33.09584502378342 22.355285278429257 48 18.787488844150182 25.892137791019092 33.54264506378411 21.777728301046622 49 19.600710200739258 24.87381861201417 34.57888907547487 20.946582111771708 50 18.94692551382948 24.554567907756926 35.157200782654805 21.341305795758796 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 478.0 1 616.0 2 754.0 3 16045.0 4 31336.0 5 21040.5 6 10745.0 7 10450.5 8 10156.0 9 10338.0 10 10520.0 11 10371.0 12 10222.0 13 9762.5 14 9303.0 15 8815.0 16 8327.0 17 7708.0 18 7089.0 19 6594.5 20 6100.0 21 5718.5 22 5337.0 23 4977.0 24 4617.0 25 4673.0 26 4729.0 27 5160.0 28 5591.0 29 5888.0 30 6185.0 31 7254.5 32 8324.0 33 9700.0 34 11076.0 35 11799.5 36 12523.0 37 14272.0 38 16021.0 39 18589.0 40 21157.0 41 24792.0 42 28427.0 43 30634.5 44 32842.0 45 33771.5 46 34701.0 47 37381.5 48 40062.0 49 41257.0 50 42452.0 51 38759.5 52 35067.0 53 30725.5 54 26384.0 55 23567.0 56 20750.0 57 19839.5 58 18929.0 59 17092.0 60 15255.0 61 12936.0 62 10617.0 63 9326.0 64 8035.0 65 6608.5 66 5182.0 67 4445.0 68 3708.0 69 3109.0 70 2510.0 71 2250.0 72 1990.0 73 1579.5 74 1169.0 75 898.5 76 628.0 77 471.0 78 314.0 79 236.0 80 158.0 81 147.0 82 136.0 83 83.0 84 30.0 85 31.0 86 32.0 87 20.5 88 9.0 89 5.5 90 2.0 91 4.0 92 6.0 93 3.0 94 0.0 95 3.0 96 6.0 97 3.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 529991.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.87396193241113 #Duplication Level Percentage of deduplicated Percentage of total 1 78.01010919121765 30.325620150426303 2 7.969225882726248 6.195907671917713 3 3.6524212304792614 4.259522516243431 4 2.2372553008501 3.478839095933272 5 1.5544811649134622 3.021442081474802 6 1.0326238740828968 2.4085308702958463 7 0.7738229279155865 2.1057094129552203 8 0.5488871389844797 1.7069934196858179 9 0.4622514411591115 1.6172590434139333 >10 3.3112860504785 26.30297373094018 >50 0.3866832104290181 10.080856503569082 >100 0.058514483293126314 3.7215820637908625 >500 0.0019504827764375437 0.5572990000277663 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 4.8762069410938593E-4 4.217464439325773 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22249 4.197995815023274 No Hit TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 889 0.16773869744957934 No Hit AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 756 0.14264393168940606 No Hit GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 675 0.12736065329411256 No Hit GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT 620 0.11698311858125894 RNA PCR Primer, Index 34 (95% over 21bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.049057436824398906 0.0 2 0.0 0.0 0.0 0.23038127062535024 0.0 3 0.0 0.0 0.0 0.31000526424033614 0.0 4 0.0 0.0 0.0 0.44887554694325 0.0 5 0.0 0.0 0.0 0.8605806513695515 0.0 6 0.0 0.0 0.0 1.1241700330760334 0.0 7 0.0 0.0 0.0 1.2841727501033036 0.0 8 0.0 0.0 0.0 1.6224803817423314 0.0 9 0.0 0.0 0.0 1.7424824195127842 0.0 10 1.8868244932461118E-4 0.0 0.0 2.070978563786932 0.0 11 1.8868244932461118E-4 0.0 0.0 2.3624929479934567 0.0 12 1.8868244932461118E-4 0.0 0.0 2.664950914260808 0.0 13 3.7736489864922235E-4 0.0 0.0 2.8157081912711726 0.0 14 3.7736489864922235E-4 0.0 0.0 2.8932566779435875 0.0 15 3.7736489864922235E-4 0.0 0.0 2.9734467189065477 0.0 16 3.7736489864922235E-4 0.0 0.0 3.1225058538729904 0.0 17 3.7736489864922235E-4 0.0 0.0 3.295905024802308 0.0 18 3.7736489864922235E-4 0.0 0.0 3.5662869746844756 0.0 19 3.7736489864922235E-4 0.0 0.0 3.683458775715059 0.0 20 7.547297972984447E-4 0.0 0.0 3.8061023677760564 0.0 21 7.547297972984447E-4 0.0 0.0 3.9527086309012796 0.0 22 7.547297972984447E-4 0.0 0.0 4.088559994414999 0.0 23 7.547297972984447E-4 0.0 0.0 4.25554396206728 0.0 24 7.547297972984447E-4 0.0 0.0 4.388753771290456 0.0 25 7.547297972984447E-4 0.0 0.0 4.493283848216291 0.0 26 7.547297972984447E-4 0.0 0.0 4.610833014145523 0.0 27 7.547297972984447E-4 0.0 0.0 4.730080322118678 0.0 28 7.547297972984447E-4 0.0 0.0 4.8549881035715705 0.0 29 7.547297972984447E-4 0.0 0.0 4.994235751173133 0.0 30 7.547297972984447E-4 0.0 0.0 5.125558735903063 0.0 31 7.547297972984447E-4 0.0 0.0 5.255749625937044 0.0 32 7.547297972984447E-4 0.0 0.0 5.394997273538607 0.0 33 7.547297972984447E-4 0.0 0.0 5.537452522778689 0.0 34 7.547297972984447E-4 0.0 0.0 5.6961344626606865 0.0 35 7.547297972984447E-4 0.0 0.0 5.872175187880549 0.0 36 7.547297972984447E-4 0.0 0.0 6.007083139147646 0.0 37 9.434122466230559E-4 0.0 0.0 6.147840246343806 0.0 38 9.434122466230559E-4 0.0 0.0 6.2965220164116 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGAC 85 0.0 44.0 25 GATACGA 20 7.852639E-4 44.0 11 TCCATCG 20 7.852639E-4 44.0 2 CGACGGT 80 0.0 44.0 28 ACCGCAG 25 4.439582E-5 44.0 21 CGCATTC 20 7.852639E-4 44.0 30 CGTTGAT 25 4.439582E-5 44.0 25 TCGGGCA 30 2.5251247E-6 44.0 5 TACGGGT 30 2.5251247E-6 44.0 4 TAACGGT 25 4.439582E-5 44.0 18 GCGCACC 20 7.852639E-4 44.0 41 CGTTAGG 95 0.0 44.0 2 CGCTCAT 20 7.852639E-4 44.0 12 CTTATCG 25 4.439582E-5 44.0 40 CCTCACG 20 7.852639E-4 44.0 21 AACGCTC 20 7.852639E-4 44.0 10 GGAACGC 25 4.439582E-5 44.0 8 AATCTCG 25 4.439582E-5 44.0 18 CGAGATA 100 0.0 44.0 19 CGGTCTA 80 0.0 44.0 31 >>END_MODULE