FastQCFastQC Report
Thu 26 May 2016
SRR1546119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546119_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences522122
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223374.278118907075358No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT9290.17792776400917795No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT7640.1463259544704111No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT6670.12774792098398458No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT6150.11778856282631263RNA PCR Primer, Index 34 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT1200.044.00000428
TCGACGG302.525052E-644.0000042
AGATACG254.4394917E-544.01
CGCGGGC351.4440593E-744.04
TTAGCGA254.4394917E-544.026
CCGTCAC207.852531E-444.040
CTAACGG207.852531E-444.02
GTAGACC207.852531E-444.010
CACTACG207.852531E-444.036
TCCGTGT207.852531E-444.042
GCGATAT207.852531E-444.09
ATGCGAG207.852531E-444.01
CGATGCA207.852531E-444.041
ACCCGAT207.852531E-444.031
AAGTGCG207.852531E-444.01
CGATAGA254.4394917E-544.010
GCGTAGT207.852531E-444.012
TACGTCA254.4394917E-544.034
ATCGACA551.8189894E-1244.018
TATCCGA254.4394917E-544.031
Produced by FastQC (version 0.11.5)