FastQCFastQC Report
Thu 26 May 2016
SRR1546118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546118_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences437820
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111962.5572152939564203No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT5350.12219633639395185No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT5040.1151158010141154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG254.438311E-544.01
CGAACTG207.851137E-444.020
CGAACGG254.438311E-544.02
GTCGAAT254.438311E-544.030
TAGCGGC207.851137E-444.03
TACGGCA254.438311E-544.04
TAACGCC207.851137E-444.012
CGTTCGA254.438311E-544.014
TCGATAG551.8189894E-1244.01
TAGCACG351.4433317E-744.01
CACTACG207.851137E-444.036
TCATGCG207.851137E-444.034
CGAATGC254.438311E-544.032
TCGTAGA207.851137E-444.015
CCGACTC302.5241116E-644.027
TATAGCG207.851137E-444.01
TAAGGTG207.851137E-444.017
CGTCGGG254.438311E-544.03
TAGTGTC207.851137E-444.033
CGCGTCA207.851137E-444.039
Produced by FastQC (version 0.11.5)