##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546118_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	437820
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.298259558722762	28.0	25.0	31.0	16.0	33.0
2	26.4865241423416	30.0	25.0	31.0	16.0	33.0
3	26.76709378283313	30.0	25.0	31.0	16.0	33.0
4	27.0253574528345	30.0	19.0	35.0	10.0	35.0
5	31.260109177287468	33.0	30.0	35.0	19.0	35.0
6	30.15132246128546	33.0	28.0	35.0	17.0	35.0
7	30.25059613539811	32.0	28.0	35.0	17.0	35.0
8	32.55881640856973	35.0	32.0	35.0	28.0	35.0
9	27.500041112786075	31.0	23.0	34.0	10.0	37.0
10	31.533557169613083	34.0	28.0	37.0	17.0	39.0
11	33.90947192910328	35.0	32.0	39.0	27.0	39.0
12	33.611598373760906	37.0	32.0	39.0	25.0	39.0
13	34.13739436298022	37.0	32.0	39.0	26.0	39.0
14	33.971380932803434	36.0	32.0	39.0	25.0	41.0
15	32.52702937280161	35.0	29.0	39.0	19.0	40.0
16	31.058919190534922	34.0	27.0	38.0	17.0	40.0
17	31.35097528664748	34.0	27.0	38.0	17.0	40.0
18	32.21439175917043	35.0	29.0	38.0	18.0	39.0
19	31.924925768580696	35.0	29.0	37.0	18.0	39.0
20	32.112196793202685	34.0	30.0	37.0	21.0	39.0
21	32.93248366908775	35.0	31.0	37.0	25.0	39.0
22	33.182287241332055	35.0	31.0	37.0	25.0	39.0
23	34.33180759216116	35.0	33.0	38.0	28.0	40.0
24	33.93175962724407	35.0	33.0	38.0	26.0	40.0
25	32.82309168151295	35.0	31.0	38.0	23.0	39.0
26	26.18183043259787	33.0	18.0	36.0	0.0	39.0
27	29.66083778721849	34.0	24.0	37.0	15.0	39.0
28	31.177470193230093	34.0	27.0	37.0	19.0	39.0
29	32.26507697227171	34.0	30.0	37.0	22.0	39.0
30	32.16248229866155	35.0	30.0	37.0	21.0	39.0
31	31.04375542460372	34.0	29.0	36.0	18.0	39.0
32	31.242428395230917	34.0	30.0	37.0	18.0	39.0
33	30.65074688228039	34.0	28.0	37.0	15.0	39.0
34	30.117253665890093	34.0	27.0	37.0	11.0	39.0
35	28.995863596911974	33.0	24.0	37.0	8.0	39.0
36	28.70284363437029	33.0	23.0	37.0	8.0	39.0
37	28.76500388287424	33.0	23.0	37.0	7.0	39.0
38	28.473272120962953	33.0	23.0	37.0	7.0	39.0
39	28.354545246905122	33.0	22.0	37.0	7.0	39.0
40	28.187275592709334	33.0	22.0	36.0	7.0	38.0
41	27.539045726554292	32.0	21.0	36.0	7.0	38.0
42	27.736807363756796	33.0	21.0	36.0	7.0	38.0
43	27.369119729569228	32.0	20.0	36.0	7.0	38.0
44	27.173900232972453	32.0	19.0	36.0	7.0	38.0
45	26.656842994838062	31.0	19.0	35.0	7.0	38.0
46	26.15371842309625	31.0	18.0	35.0	7.0	38.0
47	26.138481567767577	31.0	18.0	35.0	7.0	38.0
48	26.153588232607007	31.0	18.0	34.0	7.0	37.0
49	25.929016490795306	30.0	18.0	34.0	7.0	37.0
50	27.60516650678361	33.0	20.0	37.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	8.0
12	20.0
13	60.0
14	127.0
15	336.0
16	763.0
17	1409.0
18	2451.0
19	3867.0
20	5808.0
21	8029.0
22	10933.0
23	14089.0
24	18257.0
25	21596.0
26	25187.0
27	25426.0
28	26131.0
29	28519.0
30	32980.0
31	38036.0
32	42433.0
33	43775.0
34	40143.0
35	30370.0
36	14169.0
37	2817.0
38	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.01882051984834	21.023479968937007	23.70471883422411	32.252980676990546
2	46.90900370015075	15.949476953999362	25.300808551459504	11.840710794390388
3	26.2279018774839	12.821479146681284	48.85295326846649	12.097665707368325
4	23.275547028459183	21.428669316157325	42.762094011237494	12.533689644145996
5	16.740898085971402	25.125165593166145	45.14435156000183	12.989584760860629
6	14.662418345438766	32.08327623224156	44.53428349550043	8.720021926819241
7	69.11287743821661	2.7189255858572015	26.676031245717418	1.4921657302087616
8	70.06326800968435	2.159563290850121	26.06276552007674	1.71440317938879
9	58.868713169795804	3.1640856973185327	25.20282307797725	12.76437805490841
10	30.46046320405646	25.878671600201	33.12228769814079	10.538577497601755
11	26.466584441094515	19.39495683157462	40.97071856013887	13.167740167191996
12	24.795121282718927	17.26737015211731	44.80288703120004	13.134621533963731
13	18.2079393358001	23.76753003517427	44.22639440866109	13.798136220364535
14	13.95459321182221	28.083230551368143	43.9011465899228	14.06102964688685
15	13.120232058836963	20.388972637156822	48.636882737197936	17.853912566808276
16	14.811794801516607	15.808323055136814	49.02699739619021	20.352884747156367
17	15.337124845827052	17.06934356584898	44.15239139372345	23.44114019460052
18	16.984377141290942	21.098625005710108	45.605271572792475	16.31172628020648
19	20.325476223105383	22.4797862135124	41.66027134438811	15.534466218994108
20	21.568681193184414	20.129048467406697	41.71600200995843	16.58626832945046
21	19.558722762779222	21.492394134575854	43.529304280297836	15.419578822347082
22	17.587364670412498	18.92901192270796	42.11228358686218	21.37133982001736
23	15.555251016399433	23.12594216801425	42.37129413914394	18.947512676442376
24	15.876615960897173	21.547211182677813	47.9797633730757	14.596409483349321
25	18.399342195422776	19.661733132337492	44.529943812525694	17.40898085971404
26	12.300945594079758	36.06002466767165	34.89561920423919	16.74341053400941
27	14.997259147594901	23.021104563519255	44.70421634461651	17.277419944269333
28	14.801059796263305	22.047416746608196	47.440729066739756	15.710794390388743
29	16.082179891279523	21.7006989173633	45.39582476816957	16.82129642318761
30	17.459686629208353	21.240920926408112	45.96889132520214	15.3305011191814
31	22.979534968708602	21.59837376090631	40.48672970627199	14.935361564113107
32	21.434836234068797	21.75094787812343	40.170161253483165	16.644054634324608
33	20.282764606459274	21.035585400392858	39.34493627518158	19.336713717966287
34	16.83614270704856	22.958293362569094	39.05943081631721	21.14613311406514
35	17.918094193960986	22.97519528573386	37.90598876250514	21.200721757800007
36	23.327166415421864	23.06495820200082	34.20903567676214	19.398839705815174
37	17.333835822940934	26.787949385592253	34.22685121739528	21.651363574071535
38	19.02585537435476	28.124343337444614	32.99552327440501	19.85427801379562
39	18.6807363756795	24.163583207710932	33.208396144534284	23.94728427207528
40	19.654195788223472	22.495089306107534	36.0881184048239	21.762596500845095
41	17.580969348133937	22.630989904526974	35.42072084418254	24.36731990315655
42	18.816408569731855	24.871408341327488	31.357178749257685	24.955004339682972
43	18.94865469827783	23.37421771504271	33.11497875839386	24.562148828285597
44	18.207710931433006	24.681147503540267	34.30884838518112	22.8022931798456
45	19.2745877301174	27.17760723585035	31.701155726097486	21.84664930793477
46	20.186834772280847	24.502078479740533	32.87081448997305	22.44027225800557
47	19.252660910876614	23.90868393403682	32.61248915079256	24.226166004294004
48	18.00808551459504	25.22132383171166	33.86460189118816	22.90598876250514
49	18.894522863277146	24.820702571833174	33.78603078890868	22.498743775980998
50	18.221415193458498	24.43606961765109	35.54086154127267	21.80165364761774
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	218.0
1	378.0
2	538.0
3	9270.0
4	18002.0
5	13850.5
6	9699.0
7	9249.5
8	8800.0
9	9175.0
10	9550.0
11	9668.0
12	9786.0
13	9617.0
14	9448.0
15	9132.0
16	8816.0
17	8390.0
18	7964.0
19	7497.5
20	7031.0
21	6598.0
22	6165.0
23	5701.0
24	5237.0
25	5144.5
26	5052.0
27	5225.5
28	5399.0
29	5639.5
30	5880.0
31	6507.5
32	7135.0
33	8325.5
34	9516.0
35	9910.5
36	10305.0
37	11464.5
38	12624.0
39	14492.5
40	16361.0
41	19022.0
42	21683.0
43	23436.0
44	25189.0
45	26271.5
46	27354.0
47	28891.0
48	30428.0
49	31566.0
50	32704.0
51	30048.0
52	27392.0
53	24623.0
54	21854.0
55	19757.5
56	17661.0
57	16853.5
58	16046.0
59	14402.0
60	12758.0
61	11107.5
62	9457.0
63	8249.0
64	7041.0
65	5860.0
66	4679.0
67	4034.0
68	3389.0
69	2920.5
70	2452.0
71	2090.5
72	1729.0
73	1434.0
74	1139.0
75	879.0
76	619.0
77	479.0
78	339.0
79	250.0
80	161.0
81	136.0
82	111.0
83	79.5
84	48.0
85	39.5
86	31.0
87	22.5
88	14.0
89	12.5
90	11.0
91	8.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	437820.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.45288431450396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.45378090806514	47.38658626931903
2	6.359231154451435	7.052754190739698
3	2.406666293468349	4.003697626659492
4	1.2965351370915938	2.8758645186731875
5	0.8334621565925321	2.3108940275021337
6	0.6111454983532583	2.033386837170786
7	0.4050590947605635	1.5723186585606657
8	0.3276919308326155	1.453717018500597
9	0.2652032601896811	1.3235657136414913
>10	1.893257604897457	20.822852852978198
>50	0.1270198312342732	4.457496782585295
>100	0.01961007920805503	1.6830535090569236
>500	8.913672367297742E-4	0.2567830537312986
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.456836183648871E-4	2.767028940881251
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11196	2.5572152939564203	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	535	0.12219633639395185	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	504	0.1151158010141154	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04568087341829976	0.0
2	0.0	0.0	0.0	0.17701338449591156	0.0
3	0.0	0.0	0.0	0.2450778858891782	0.0
4	0.0	0.0	0.0	0.3227353707002878	0.0
5	0.0	0.0	0.0	0.5803754967794984	0.0
6	0.0	0.0	0.0	0.7151340733634827	0.0
7	0.0	0.0	0.0	0.8026129459595267	0.0
8	0.0	0.0	0.0	0.9862500571010918	0.0
9	0.0	0.0	0.0	1.0798958476086062	0.0
10	0.0	0.0	0.0	1.2882006303960531	0.0
11	0.0	0.0	0.0	1.493307752044219	0.0
12	0.0	0.0	0.0	1.6904207208441826	0.0
13	0.0	0.0	0.0	1.801882051984834	0.0
14	0.0	0.0	0.0	1.856927504453885	0.0
15	0.0	0.0	0.0	1.912658170024211	0.0
16	0.0	0.0	0.0	2.0033347037595357	0.0
17	0.0	0.0	0.0	2.1399205152802523	0.0
18	0.0	0.0	0.0	2.3226440089534512	0.0
19	0.0	0.0	0.0	2.3929925540176327	0.0
20	0.0	0.0	0.0	2.4640263121830888	0.0
21	0.0	0.0	0.0	2.55104837604495	0.0
22	0.0	0.0	0.0	2.638070439906811	0.0
23	0.0	0.0	0.0	2.7308026129459595	0.0
24	0.0	0.0	0.0	2.8100589283267094	0.0
25	0.0	0.0	0.0	2.8678452332008586	0.0
26	0.0	0.0	0.0	2.935681330227034	0.0
27	0.0	0.0	0.0	3.021332967886346	0.0
28	0.0	0.0	0.0	3.1099538623178473	0.0
29	0.0	0.0	0.0	3.211365401306473	0.0
30	0.0	0.0	0.0	3.312548535928007	0.0
31	0.0	0.0	0.0	3.4217258233977432	0.0
32	0.0	0.0	0.0	3.525649810424375	0.0
33	0.0	0.0	0.0	3.622493262071171	0.0
34	0.0	0.0	0.0	3.7307569320725413	0.0
35	0.0	0.0	0.0	3.872596044036362	0.0
36	0.0	0.0	0.0	3.974235987392079	0.0
37	0.0	0.0	0.0	4.086154127266913	0.0
38	0.0	0.0	0.0	4.2058380156228585	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	25	4.438311E-5	44.0	1
CGAACTG	20	7.851137E-4	44.0	20
CGAACGG	25	4.438311E-5	44.0	2
GTCGAAT	25	4.438311E-5	44.0	30
TAGCGGC	20	7.851137E-4	44.0	3
TACGGCA	25	4.438311E-5	44.0	4
TAACGCC	20	7.851137E-4	44.0	12
CGTTCGA	25	4.438311E-5	44.0	14
TCGATAG	55	1.8189894E-12	44.0	1
TAGCACG	35	1.4433317E-7	44.0	1
CACTACG	20	7.851137E-4	44.0	36
TCATGCG	20	7.851137E-4	44.0	34
CGAATGC	25	4.438311E-5	44.0	32
TCGTAGA	20	7.851137E-4	44.0	15
CCGACTC	30	2.5241116E-6	44.0	27
TATAGCG	20	7.851137E-4	44.0	1
TAAGGTG	20	7.851137E-4	44.0	17
CGTCGGG	25	4.438311E-5	44.0	3
TAGTGTC	20	7.851137E-4	44.0	33
CGCGTCA	20	7.851137E-4	44.0	39
>>END_MODULE