FastQCFastQC Report
Thu 26 May 2016
SRR1546117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546117_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences765338
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208282.721411977453099No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT35360.46201808873987704No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT24120.31515487274903375No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT22780.2976462687074208No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC22160.28954527280757No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT21900.2861480809786003No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19840.25923186879522514No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATG16460.21506837501861922No Hit
GAACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC13610.17782992612414383No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC10700.1398075098845216No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT9630.12582675889606945No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT9580.12517345277511374No Hit
GGGCAGGGGCTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT8670.11328328137371986No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT7900.10322236711100194No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT7860.10269972221423737No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGG207.854831E-444.02
TATTACG254.441441E-544.01
CGTACAT207.854831E-444.035
TCGTAAG254.441441E-544.01
GCTACGA207.854831E-444.010
GCGTAAG207.854831E-444.01
CGTTTTT117400.042.9505961
TAGCCGT1400.042.4285744
CGGTCTA1900.041.6842131
CGTAAGG750.041.0666662
CGGGTAT700.040.857146
AGGGTAC6800.040.7647066
GCCCGAA1950.040.61538725
TAAGGGC2900.040.2068944
GTTTTTT129350.039.8840332
GCGGGAT1600.039.8755
TCGCTAT501.3460522E-939.60000223
GTTGATC12500.039.24816
TCGTTTA452.3492248E-839.1111138
CATATGC12150.038.9300433
Produced by FastQC (version 0.11.5)