##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546115_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	760780
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.586077446830885	30.0	28.0	31.0	16.0	33.0
2	28.691638844343963	31.0	28.0	33.0	16.0	33.0
3	28.231189042824468	31.0	27.0	33.0	16.0	34.0
4	32.1368135334788	35.0	32.0	35.0	25.0	37.0
5	33.661983753516125	35.0	33.0	35.0	28.0	37.0
6	33.5061528957123	35.0	33.0	37.0	28.0	37.0
7	34.1206906070086	35.0	33.0	37.0	30.0	37.0
8	34.47713530849917	35.0	35.0	37.0	31.0	37.0
9	36.262818423197245	39.0	35.0	39.0	31.0	39.0
10	35.962112568679515	37.0	35.0	39.0	31.0	39.0
11	36.23651646993875	38.0	35.0	39.0	31.0	39.0
12	35.3208693709088	37.0	34.0	39.0	29.0	39.0
13	35.83166749914562	37.0	35.0	39.0	30.0	39.0
14	36.644936775414706	38.0	35.0	40.0	31.0	41.0
15	36.88699755514077	38.0	35.0	40.0	31.0	41.0
16	36.46959567811982	38.0	35.0	40.0	31.0	41.0
17	35.93259549409816	38.0	34.0	40.0	30.0	41.0
18	35.58239569915087	38.0	34.0	39.0	29.0	40.0
19	35.03245879229212	37.0	33.0	39.0	27.0	40.0
20	35.2741134099214	36.0	34.0	39.0	29.0	40.0
21	35.56223349720024	36.0	34.0	39.0	30.0	40.0
22	35.87402665685218	37.0	34.0	40.0	31.0	41.0
23	36.18791372012934	37.0	35.0	40.0	31.0	41.0
24	35.80556928415574	37.0	35.0	40.0	31.0	41.0
25	35.53505744104734	37.0	34.0	40.0	30.0	41.0
26	35.60428376140277	37.0	34.0	40.0	30.0	41.0
27	35.95354241699309	37.0	35.0	40.0	31.0	41.0
28	35.83865375009858	37.0	35.0	40.0	30.0	41.0
29	35.84459239201872	37.0	35.0	40.0	30.0	41.0
30	35.46701411709036	37.0	35.0	40.0	29.0	41.0
31	34.74698335918399	36.0	34.0	40.0	26.0	41.0
32	34.70147611661716	37.0	34.0	40.0	25.0	41.0
33	34.35299955309025	37.0	34.0	40.0	22.0	41.0
34	33.88426089013907	37.0	33.0	40.0	19.0	41.0
35	33.54257998370094	37.0	33.0	40.0	16.0	41.0
36	33.205598201845476	37.0	33.0	40.0	15.0	41.0
37	33.18436735981493	37.0	33.0	40.0	15.0	41.0
38	32.96915534057152	37.0	32.0	40.0	14.0	41.0
39	32.697356660269726	36.0	32.0	40.0	12.0	41.0
40	32.51611898314887	36.0	31.0	40.0	11.0	40.0
41	32.111018954231184	35.0	31.0	39.0	10.0	40.0
42	32.35539446357686	36.0	31.0	40.0	10.0	41.0
43	32.19676253318962	35.0	31.0	40.0	10.0	40.0
44	32.060750808380874	35.0	31.0	39.0	10.0	40.0
45	31.94844370251584	35.0	31.0	39.0	10.0	40.0
46	31.733813980388547	35.0	30.0	39.0	10.0	40.0
47	31.697791740056257	35.0	30.0	39.0	10.0	40.0
48	31.628236809590156	35.0	30.0	39.0	10.0	40.0
49	31.633704881831804	35.0	30.0	39.0	10.0	40.0
50	31.773524540603066	35.0	30.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	3.0
11	8.0
12	8.0
13	17.0
14	47.0
15	95.0
16	248.0
17	482.0
18	1034.0
19	1839.0
20	2746.0
21	4086.0
22	5821.0
23	8173.0
24	11690.0
25	17210.0
26	23344.0
27	25977.0
28	24851.0
29	23457.0
30	25059.0
31	29338.0
32	37049.0
33	51200.0
34	73337.0
35	71719.0
36	78965.0
37	105865.0
38	113095.0
39	24015.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.84295065590578	20.46386603223008	22.94750124871842	25.74568206314572
2	43.70816793291096	20.29338310681143	24.335813244301903	11.662635715975709
3	29.367885591103864	19.169667972344172	38.668866163674124	12.793580272877836
4	25.85346617944741	22.706958647703672	37.23073687531218	14.208838297536738
5	19.971739530481873	26.315623439101977	37.125844527984434	16.586792502431717
6	17.807513341570495	36.75175477799101	35.71006072714845	9.730671153290045
7	69.37511501353873	5.796156576145535	21.664738820684036	3.1639895896316936
8	69.50761061016325	5.765398669786272	20.610951917768606	4.116038802281868
9	66.21927495465181	5.881069428744183	21.695365282999028	6.204290333604985
10	33.094192802124134	24.860274980940613	28.422671468755752	13.6228607481795
11	28.2105207813034	22.50558637188149	31.928152685401823	17.355740161413287
12	25.30087541733484	19.693078156628722	38.83054233812666	16.175504087909776
13	20.81363863403349	24.300717684481715	38.846447067483375	16.03919661400142
14	17.076684455427323	30.975446252530297	37.043560556271196	14.904308735771183
15	16.520807592207998	24.12497699729225	43.22261363337627	16.13160177712348
16	18.30489760508951	18.866558006256735	44.33173847892952	18.49680590972423
17	17.380583085780383	19.260495806934987	37.844449118010466	25.514471989274167
18	19.030205841373327	23.60537869029154	38.551618076184965	18.812797392150163
19	22.82118352217461	25.54509845158916	33.48668471831541	18.147033307920818
20	23.020978469465547	23.001524750913536	33.56541970083335	20.41207707878756
21	22.618628249953996	23.538999447935012	36.90883041089408	16.93354189121691
22	19.239859091984542	20.04797707615868	36.13528221036305	24.57688162149373
23	18.577775440994767	26.50358842240858	34.58871158547806	20.329924551118587
24	19.369200031546573	25.481873866295118	38.44712006099004	16.701806041168275
25	23.135860564157838	21.998212360997922	36.17300665106864	18.6929204237756
26	16.936433660190854	23.865769341991115	35.178763900207684	24.019033097610347
27	18.04043218801756	23.176608217881647	38.55582428560162	20.22713530849917
28	17.19669286784616	23.564236704434922	41.178527300927996	18.060543126790925
29	18.493256920528932	25.00814953074476	37.77636110307842	18.722232445647887
30	19.007334577670285	22.679355398406898	40.2531612292647	18.060148794658115
31	24.928494439916925	23.440942190909333	33.29096453639686	18.339598832776886
32	22.696968900339126	25.517363758248113	33.14256421041563	18.643103130997137
33	21.40750282604695	22.470753700149846	32.10455059281264	24.017192880990564
34	18.862746128972894	24.710954546649493	32.518862220352794	23.90743710402482
35	19.547963931754254	23.138095112910435	33.11206919214491	24.20187176319041
36	25.36278556218618	24.63274534030863	29.88091169589106	20.123557401614136
37	19.768264149951364	26.578117195509872	31.00462683035832	22.64899182418045
38	20.63513762191435	29.59541523173585	28.239964247219955	21.52948289912984
39	20.086884513262703	25.03062646231499	29.004179920607797	25.878309103814505
40	20.260653539788112	23.41557349036515	34.60855963616288	21.71521333368385
41	19.108283603669918	23.31396724414417	34.233418333815294	23.344330818370622
42	20.949420331764767	27.310129078051474	28.721838113515076	23.018612476668682
43	20.212545019585164	24.676253318962118	29.908777833276375	25.202423828176347
44	20.17705512763217	24.520886458634557	33.040826520150375	22.261231893582902
45	21.749125897105603	26.442729829911404	30.091222166723625	21.716922106259364
46	23.84224085806672	24.871710612792132	30.121980073082888	21.164068456058256
47	22.40910644338705	26.168406109519175	30.052971949840952	21.36951549725282
48	19.3761665658929	28.42214569257867	30.48713162806593	21.7145561134625
49	19.266673677015696	27.209180052051842	31.981256079287046	21.542890191645416
50	19.793632850495545	24.277977864822944	34.60225032203791	21.326138962643604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2191.0
1	2093.0
2	1995.0
3	16887.5
4	31780.0
5	21489.0
6	11198.0
7	11010.5
8	10823.0
9	10941.5
10	11060.0
11	10889.5
12	10719.0
13	10222.5
14	9726.0
15	9128.0
16	8530.0
17	7902.0
18	7274.0
19	6696.0
20	6118.0
21	5865.5
22	5613.0
23	5463.5
24	5314.0
25	5361.5
26	5409.0
27	5841.0
28	6273.0
29	6728.0
30	7183.0
31	8166.5
32	9150.0
33	10934.0
34	12718.0
35	14762.0
36	16806.0
37	18332.5
38	19859.0
39	23334.0
40	26809.0
41	31398.5
42	35988.0
43	40475.5
44	44963.0
45	47760.5
46	50558.0
47	58885.5
48	67213.0
49	69991.0
50	72769.0
51	66081.0
52	59393.0
53	50503.0
54	41613.0
55	37393.5
56	33174.0
57	31494.0
58	29814.0
59	27957.5
60	26101.0
61	23486.0
62	20871.0
63	18262.5
64	15654.0
65	13092.0
66	10530.0
67	9430.0
68	8330.0
69	7213.0
70	6096.0
71	5331.5
72	4567.0
73	3935.0
74	3303.0
75	2439.5
76	1576.0
77	1191.0
78	806.0
79	611.5
80	417.0
81	333.0
82	249.0
83	193.5
84	138.0
85	92.5
86	47.0
87	38.0
88	29.0
89	21.0
90	13.0
91	12.0
92	11.0
93	7.5
94	4.0
95	3.0
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	760780.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.54240112648608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.32348464706504	26.018540209236363
2	7.75965381258237	5.360741491937745
3	3.663315468689645	3.796191371170172
4	2.397615356147639	3.3127756551629806
5	1.6890650469691089	2.9172181190567
6	1.2957350230119902	2.6854679351110096
7	1.034992284440009	2.5025783056361477
8	0.8185973706179177	2.2621054989576717
9	0.6612776067735497	2.0557904714221213
>10	5.065413963687561	34.36880943576677
>50	0.2371305305902976	5.117025432593466
>100	0.04374252424593433	2.9316829526117654
>500	0.005371888942483163	1.1649062557305847
>1k	0.004220769883379627	2.6992053587385776
>5k	0.0	0.0
>10k+	3.8370635303451155E-4	2.80696150686794
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21178	2.7837219695575595	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT	3525	0.4633402560529983	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2368	0.31125949683219856	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2244	0.29496043534267463	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2199	0.2890454533505087	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC	2116	0.27813559767606927	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1787	0.2348905071111228	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATG	1606	0.21109913509818873	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	1416	0.18612476668682143	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	1064	0.13985646310365676	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT	1021	0.13420436920003156	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1019	0.13394148111149085	No Hit
GGGCAGGGGCTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	836	0.10988722101001605	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.030495018270722155	0.0
2	0.0	0.0	0.0	0.1848103262441179	0.0
3	0.0	0.0	0.0	0.2500065722022135	0.0
4	0.0	0.0	0.0	0.537869029154289	0.0
5	1.314440442703541E-4	0.0	0.0	1.2174347380320198	0.0
6	1.314440442703541E-4	0.0	0.0	1.5695733326322985	0.0
7	1.314440442703541E-4	0.0	0.0	1.7918452114934673	0.0
8	1.314440442703541E-4	0.0	0.0	2.192223770340966	0.0
9	1.314440442703541E-4	0.0	0.0	2.3414127605878177	0.0
10	2.628880885407082E-4	0.0	0.0	2.8519414285338733	0.0
11	2.628880885407082E-4	0.0	0.0	3.0313625489629064	0.0
12	3.9433213281106234E-4	0.0	0.0	3.412024501169852	0.0
13	3.9433213281106234E-4	0.0	0.0	3.5228318304897606	0.0
14	5.257761770814164E-4	0.0	0.0	3.5810615421015273	0.0
15	6.572202213517706E-4	0.0	0.0	3.6553274271142775	0.0
16	6.572202213517706E-4	0.0	0.0	3.739057283314493	0.0
17	6.572202213517706E-4	0.0	0.0	3.817792265832435	0.0
18	7.886642656221247E-4	0.0	0.0	3.9530481873866297	0.0
19	7.886642656221247E-4	0.0	0.0	4.029417177107705	0.0
20	0.0010515523541628328	0.0	0.0	4.091853098136124	0.0
21	0.0010515523541628328	0.0	0.0	4.164410210573359	0.0
22	0.0010515523541628328	0.0	0.0	4.252872052367307	0.0
23	0.001182996398433187	0.0	0.0	4.347906096374773	0.0
24	0.001182996398433187	0.0	0.0	4.41717710770525	0.0
25	0.001182996398433187	0.0	0.0	4.470017613501932	0.0
26	0.001182996398433187	0.0	0.0	4.542574725939168	0.0
27	0.001182996398433187	0.0	0.0	4.622755592944084	0.0
28	0.001182996398433187	0.0	0.0	4.705959672967218	0.0
29	0.001182996398433187	0.0	0.0	4.785088987617971	0.0
30	0.001182996398433187	0.0	0.0	4.881963248245222	0.0
31	0.001182996398433187	0.0	0.0	4.964904440179815	0.0
32	0.001182996398433187	0.0	0.0	5.0494229606456535	0.0
33	0.001182996398433187	0.0	0.0	5.1595730697442095	0.0
34	0.001182996398433187	0.0	0.0	5.262493756407897	0.0
35	0.001182996398433187	0.0	0.0	5.395909461342306	0.0
36	0.001182996398433187	0.0	0.0	5.483056862693552	0.0
37	0.001182996398433187	0.0	0.0	5.575724913904151	0.0
38	0.001182996398433187	0.0	0.0	5.693235889481848	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGAC	20	7.854802E-4	44.0	15
TAGCACG	20	7.854802E-4	44.0	1
TCGTAAG	20	7.854802E-4	44.0	1
TCGACGG	20	7.854802E-4	44.0	2
TACGTAG	40	8.305506E-9	44.0	1
CGTTTTT	10935	0.0	43.215366	1
TACGGGA	105	0.0	41.90476	4
TCGTTTA	65	0.0	40.615387	38
ATAGCGG	65	0.0	40.615387	2
GCGATAT	60	3.6379788E-12	40.333332	9
TGCGTAG	60	3.6379788E-12	40.333332	1
CGGTCTA	225	0.0	40.08889	31
CGTAAGG	110	0.0	40.0	2
TACGGGC	105	0.0	39.80952	4
GCGTAAG	50	1.3460522E-9	39.600002	1
TAACGGG	140	0.0	39.285713	3
AGGGTAC	575	0.0	39.02609	6
GTTTTTT	12370	0.0	38.94907	2
ATAGGGC	255	0.0	38.823532	4
CGGGTAT	40	4.1220846E-7	38.5	6
>>END_MODULE