FastQCFastQC Report
Thu 26 May 2016
SRR1546114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546114_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences634815
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106891.6837976418326601No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT19610.3089088947173586No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT14780.23282373604908516No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT14310.22542000425320763No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT12960.20415396611611256No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10470.16492994021880392No Hit
GAATGATACGGCTGCCTCTTATACACATCTGACGCCCTACGCATCGTATG9390.14791710970912786No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC6790.106960295519167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATT650.044.0000049
TCGATAG302.5259178E-644.0000041
TACGTAG302.5259178E-644.0000041
TCGTTTA351.4447141E-744.038
ACGTTAG207.853815E-444.01
TCACTCG502.7284841E-1144.021
CTCGAAT351.4447141E-744.022
GACTCGA207.853815E-444.09
CGTTTTT99800.043.3166351
ATTATGA4850.042.18556626
AGGGTAC4700.040.7234086
ACGGGCT1300.040.6153875
GTTTTTT110400.040.0543482
CGACGGT2600.039.76923428
CGGTCTA2600.039.76923431
CGTTCGA950.039.3684214
TACGAGG1400.039.2857132
CGTTCAC452.3481334E-839.11110721
AGGGTAA2600.038.923086
AGATTAT5200.038.9230824