FastQCFastQC Report
Thu 26 May 2016
SRR1546113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546113_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1291642
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369642.8617836830948513No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT18030.13958976248836752No Hit
GAATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT16890.13076378748910303No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT16610.12859600415595032No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCC16580.1283637416559697No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT13820.10699559165775036No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC1450.044.00000425
CGAGTTA207.8568433E-444.00000415
TCGATAG408.314601E-944.0000041
TAGCACG207.8568433E-444.0000041
CTACGGT408.314601E-944.00000440
CCGAACG207.8568433E-444.00000437
ATCGAGT207.8568433E-444.00000425
ACGCCCG302.5279624E-644.029
CGACGGT1400.044.028
CGCATTA254.443147E-544.031
TATTCCG302.5279624E-644.01
TGTTACG302.5279624E-644.01
GTCCGAA302.5279624E-644.035
CGTAATC600.044.022
ACGTACT254.443147E-544.029
CGTTTTT225800.043.444641
CGGTCTA1500.041.06666631
TGTACGG1250.040.482
TCGCGCA1800.040.33333234
CGTAAGG2350.040.2553182
Produced by FastQC (version 0.11.5)