##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546113_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1291642
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.984070663542994	31.0	28.0	33.0	23.0	33.0
2	28.980552660876622	31.0	28.0	33.0	16.0	33.0
3	28.40693551309109	31.0	28.0	33.0	16.0	34.0
4	32.140074416904994	35.0	32.0	35.0	25.0	37.0
5	33.729304249939226	35.0	33.0	35.0	30.0	37.0
6	33.58458922828462	35.0	33.0	37.0	28.0	37.0
7	34.21367298369053	35.0	33.0	37.0	30.0	37.0
8	34.688003332192665	35.0	35.0	37.0	32.0	37.0
9	36.540073023329995	39.0	37.0	39.0	32.0	39.0
10	36.26937417643589	38.0	35.0	39.0	31.0	39.0
11	36.439963240588334	39.0	35.0	39.0	32.0	39.0
12	35.455808188337016	37.0	35.0	39.0	30.0	39.0
13	35.698441208941794	37.0	35.0	39.0	30.0	39.0
14	36.46222560121148	38.0	35.0	40.0	30.0	41.0
15	36.95439603233714	39.0	35.0	40.0	31.0	41.0
16	36.63467199115544	38.0	35.0	40.0	31.0	41.0
17	36.18062048152662	38.0	34.0	40.0	30.0	41.0
18	35.75861577743678	38.0	34.0	39.0	29.0	40.0
19	35.219126507190076	37.0	34.0	39.0	29.0	41.0
20	35.41714344996524	35.0	34.0	39.0	30.0	41.0
21	35.63999312503	36.0	34.0	39.0	31.0	41.0
22	35.928189854464314	36.0	35.0	40.0	31.0	41.0
23	36.2351541681054	37.0	35.0	40.0	32.0	41.0
24	35.904309398424644	36.0	35.0	40.0	31.0	41.0
25	35.56319010995307	36.0	34.0	40.0	30.0	41.0
26	35.63082572415576	36.0	34.0	40.0	31.0	41.0
27	35.94838817567097	37.0	35.0	40.0	31.0	41.0
28	35.84772096292936	36.0	35.0	40.0	31.0	41.0
29	35.9236351868397	37.0	35.0	40.0	31.0	41.0
30	35.545308220079555	36.0	35.0	40.0	30.0	41.0
31	34.836376488221966	36.0	34.0	40.0	27.0	41.0
32	34.64229020115481	36.0	34.0	40.0	25.0	41.0
33	34.1728334941106	36.0	33.0	40.0	22.0	41.0
34	33.66445346311129	36.0	33.0	40.0	18.0	41.0
35	33.27395748976884	36.0	33.0	40.0	15.0	41.0
36	32.85384495084551	36.0	33.0	40.0	12.0	41.0
37	32.75206907177066	36.0	33.0	40.0	10.0	41.0
38	32.597784060908516	36.0	32.0	40.0	10.0	41.0
39	32.4195582057567	36.0	32.0	40.0	10.0	41.0
40	32.25194597264567	35.0	31.0	40.0	10.0	40.0
41	31.9632150394614	35.0	31.0	39.0	10.0	40.0
42	32.12833819278097	35.0	31.0	40.0	10.0	40.0
43	31.92636194858947	35.0	31.0	39.0	10.0	40.0
44	31.677622746860198	35.0	31.0	39.0	10.0	40.0
45	31.650287773237476	35.0	30.0	39.0	10.0	40.0
46	31.515783011082018	35.0	30.0	39.0	10.0	40.0
47	31.48702117150108	35.0	30.0	39.0	10.0	40.0
48	31.479725032168357	35.0	30.0	39.0	10.0	40.0
49	31.449138383545904	35.0	30.0	39.0	10.0	40.0
50	31.635546072363706	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	5.0
12	17.0
13	18.0
14	72.0
15	150.0
16	440.0
17	847.0
18	1608.0
19	2840.0
20	4340.0
21	6546.0
22	9471.0
23	13369.0
24	20085.0
25	30307.0
26	42732.0
27	47669.0
28	43025.0
29	39529.0
30	41111.0
31	47664.0
32	60742.0
33	89274.0
34	139600.0
35	113814.0
36	119276.0
37	171281.0
38	200173.0
39	45634.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.739232697605065	21.03547267741371	23.944637910504614	26.280656714476613
2	44.13692029215526	18.852592281762284	25.266134114561154	11.744353311521303
3	28.11545304349038	17.90829037767431	41.634137013197154	12.342119565638157
4	24.393678743800525	22.857184885595235	39.00105447174991	13.748081898854325
5	19.936483948338626	26.947327510254386	39.08435928840964	14.031829252997346
6	17.98888546516759	34.82706508459775	37.84957441767921	9.33447503255546
7	71.27145137739404	4.60715894961607	21.77662231485195	2.344767358137936
8	71.88439211484297	3.9400236288383317	21.385260002384562	2.7903242539341395
9	68.21696724014859	5.165595420402867	22.636458089780295	3.980979249668252
10	36.38190768030151	25.323580372889705	27.89844244767513	10.39606949913366
11	32.18980181815085	20.210321435815807	34.712946776273924	12.886929969759423
12	28.93394609342217	20.155739748320357	38.09012094682582	12.820193211431652
13	19.364808515052935	28.889661376759197	38.786056817601164	12.95947329058671
14	14.513541677957203	32.345262851471226	39.663467121694715	13.477728348876855
15	13.913220536340564	23.113138160573904	49.69287155419226	13.28076974889327
16	16.733506652772206	18.739403023438385	48.946225037587816	15.580865286201595
17	16.30536944447455	18.61707810678191	39.86096766751159	25.216584781231948
18	18.68784074844268	21.70810487735766	42.422435938131464	17.1816184360682
19	22.420376543964966	22.963174006419734	37.91437565517381	16.702073794441493
20	23.370639852219114	21.888495419009292	37.61855065103179	17.122314077739805
21	20.278064664976828	23.5203717438733	39.73779112168852	16.46377246946135
22	18.515656815123695	20.70751802744104	38.87029068426081	21.906534473174453
23	16.481811523626515	25.48848674787596	38.46739266762772	19.562309060869808
24	16.734435702772128	23.090376435575802	44.56838659628597	15.6068012653661
25	17.595897315200343	22.138177606488483	42.34439573813797	17.921529340173205
26	15.268085119560993	26.408401089465965	38.923711059256355	19.399802731716683
27	15.874832190343763	26.61681797278193	40.621704775781524	16.886645061092782
28	14.097946644658505	24.315328860473723	45.07975120041002	16.50697329445775
29	15.082042857076496	23.759447277186712	43.41218387138232	17.74632599435447
30	17.254935965228754	24.49889365629176	41.98129202983489	16.264878348644594
31	23.53004934803916	23.009781348082516	36.52087807612326	16.93929122775506
32	23.219824068898347	24.22226901881481	36.7928574636006	15.765049448686247
33	21.86674016484444	24.117208947990232	35.16647801790279	18.849572869262534
34	17.52490241103959	25.253591939562202	36.01655876783195	21.204946881566254
35	17.90929684850756	25.465183077044568	35.864349409511306	20.761170664936568
36	24.94383118542135	25.154415852070468	32.458916634795095	17.442836327713096
37	18.518831069290098	29.34195388505484	33.01046265141579	19.12875239423927
38	19.170327381735806	30.806523789099455	30.80590442243284	19.217244406731897
39	19.414899794215426	26.973263489418898	32.15751733065354	21.45431938571214
40	20.969974652419168	24.55270113545394	33.74379278468801	20.733531427438873
41	17.502295527708142	24.152899952153923	34.1411939221549	24.203610597983033
42	20.20095351498325	25.56443658536963	31.07176756407735	23.162842335569763
43	19.90628982334114	24.865403881261216	31.72326387652306	23.50504241887458
44	18.69449894010879	27.106504743574455	33.25534474722872	20.94365156908803
45	18.424842177631263	30.401845093299844	30.256603609978615	20.916709119090275
46	20.84749489409604	27.748865397687595	31.834672455680447	19.56896725253592
47	19.934548427505455	26.006277281166145	32.5456279681212	21.513546323207205
48	19.80804278584933	26.414672176965443	33.62464212219795	20.15264291498728
49	19.881205473343233	24.848758402095935	34.728740626272604	20.541295498288225
50	17.8584313610118	28.056148685161986	33.14563942640453	20.939780527421682
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	695.0
1	1331.5
2	1968.0
3	29089.5
4	56211.0
5	38475.0
6	20739.0
7	20498.5
8	20258.0
9	20851.5
10	21445.0
11	21315.5
12	21186.0
13	20191.5
14	19197.0
15	18057.5
16	16918.0
17	15719.0
18	14520.0
19	13778.5
20	13037.0
21	12168.5
22	11300.0
23	11743.0
24	12186.0
25	12811.0
26	13436.0
27	14493.5
28	15551.0
29	17598.0
30	19645.0
31	21293.0
32	22941.0
33	26475.5
34	30010.0
35	34354.5
36	38699.0
37	41750.5
38	44802.0
39	51440.5
40	58079.0
41	68978.0
42	79877.0
43	93851.0
44	107825.0
45	108561.5
46	109298.0
47	106809.0
48	104320.0
49	99933.5
50	95547.0
51	86370.5
52	77194.0
53	66570.5
54	55947.0
55	51758.0
56	47569.0
57	43278.0
58	38987.0
59	34820.5
60	30654.0
61	27570.5
62	24487.0
63	20255.5
64	16024.0
65	13221.5
66	10419.0
67	8637.0
68	6855.0
69	5780.5
70	4706.0
71	4212.5
72	3719.0
73	3087.5
74	2456.0
75	1942.5
76	1429.0
77	1141.0
78	853.0
79	590.0
80	327.0
81	240.0
82	153.0
83	122.5
84	92.0
85	63.0
86	34.0
87	27.0
88	20.0
89	17.0
90	14.0
91	10.5
92	7.0
93	6.0
94	5.0
95	2.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1291642.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.21644776141609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.99449205194078	23.079384504751264
2	7.02480030746953	4.104794224351264
3	2.985760345884913	2.616999335209626
4	1.7311973240988054	2.023177447369443
5	1.214874702223886	1.7747161637095048
6	0.9058930175485921	1.5880185614784017
7	0.6858602162240709	1.4026879425260879
8	0.572606209694733	1.3383615530726907
9	0.4501043928916161	1.1835406333891625
>10	4.286603605091168	27.765317515624606
>50	0.8657189727996402	17.68740950528436
>100	0.27729414273234065	11.274695765960589
>500	0.0026637285554990554	0.4693606824259452
>1k	0.0018646099888493385	0.8148244644337002
>5k	0.0	0.0
>10k+	2.663728555499055E-4	2.876711700413304
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36964	2.8617836830948513	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1803	0.13958976248836752	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	1689	0.13076378748910303	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1661	0.12859600415595032	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCC	1658	0.1283637416559697	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1382	0.10699559165775036	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.548416666537632E-4	0.0	0.0	0.04536860832955262	0.0
2	1.548416666537632E-4	0.0	0.0	0.18457126665128573	0.0
3	1.548416666537632E-4	0.0	0.0	0.24310141664640822	0.0
4	2.3226249998064478E-4	0.0	0.0	0.39445914580046176	0.0
5	2.3226249998064478E-4	0.0	0.0	0.7572531707702289	0.0
6	2.3226249998064478E-4	0.0	0.0	0.9920705582506608	0.0
7	2.3226249998064478E-4	0.0	0.0	1.1589124540700906	0.0
8	2.3226249998064478E-4	0.0	0.0	1.5068416790410966	0.0
9	2.3226249998064478E-4	0.0	0.0	1.6450378665295802	0.0
10	2.3226249998064478E-4	0.0	0.0	2.006670578999444	0.0
11	3.096833333075264E-4	0.0	0.0	2.2383911331468007	0.0
12	3.096833333075264E-4	0.0	0.0	2.5300354122891635	0.0
13	3.8710416663440797E-4	0.0	0.0	2.632540595613955	0.0
14	3.8710416663440797E-4	0.0	0.0	2.6738058997771827	0.0
15	3.8710416663440797E-4	0.0	0.0	2.739768449771686	0.0
16	3.8710416663440797E-4	0.0	0.0	2.846376937262802	0.0
17	4.6452499996128957E-4	0.0	0.0	2.985734437251189	0.0
18	4.6452499996128957E-4	0.0	0.0	3.1908996455674252	0.0
19	5.419458332881712E-4	0.0	0.0	3.2854304830595473	0.0
20	6.967874999419343E-4	0.0	0.0	3.3853807788845516	0.0
21	8.516291665956975E-4	0.0	0.0	3.4991894038750675	0.0
22	8.516291665956975E-4	0.0	0.0	3.6228304746980973	0.0
23	8.516291665956975E-4	0.0	0.0	3.748174803854319	0.0
24	8.516291665956975E-4	0.0	0.0	3.8531574538455704	0.0
25	8.516291665956975E-4	0.0	0.0	3.9353783788387187	0.0
26	8.516291665956975E-4	0.0	0.0	4.020309032998307	0.0
27	8.516291665956975E-4	0.0	0.0	4.1122849829906425	0.0
28	8.516291665956975E-4	0.0	0.0	4.209602970482533	0.0
29	9.290499999225791E-4	0.0	0.0	4.317992137140167	0.0
30	9.290499999225791E-4	0.0	0.0	4.45696253296192	0.0
31	9.290499999225791E-4	0.0	0.0	4.57340346628555	0.0
32	9.290499999225791E-4	0.0	0.0	4.680708741276607	0.0
33	9.290499999225791E-4	0.0	0.0	4.798310987100141	0.0
34	9.290499999225791E-4	0.0	0.0	4.919939116256672	0.0
35	9.290499999225791E-4	0.0	0.0	5.072303316243975	0.0
36	9.290499999225791E-4	0.0	0.0	5.183711895401357	0.0
37	9.290499999225791E-4	0.0	0.0	5.2992237787250644	0.0
38	9.290499999225791E-4	0.0	0.0	5.437342545380221	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	145	0.0	44.000004	25
CGAGTTA	20	7.8568433E-4	44.000004	15
TCGATAG	40	8.314601E-9	44.000004	1
TAGCACG	20	7.8568433E-4	44.000004	1
CTACGGT	40	8.314601E-9	44.000004	40
CCGAACG	20	7.8568433E-4	44.000004	37
ATCGAGT	20	7.8568433E-4	44.000004	25
ACGCCCG	30	2.5279624E-6	44.0	29
CGACGGT	140	0.0	44.0	28
CGCATTA	25	4.443147E-5	44.0	31
TATTCCG	30	2.5279624E-6	44.0	1
TGTTACG	30	2.5279624E-6	44.0	1
GTCCGAA	30	2.5279624E-6	44.0	35
CGTAATC	60	0.0	44.0	22
ACGTACT	25	4.443147E-5	44.0	29
CGTTTTT	22580	0.0	43.44464	1
CGGTCTA	150	0.0	41.066666	31
TGTACGG	125	0.0	40.48	2
TCGCGCA	180	0.0	40.333332	34
CGTAAGG	235	0.0	40.255318	2
>>END_MODULE