FastQCFastQC Report
Thu 26 May 2016
SRR1546112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546112_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1286513
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379392.948979139736637No Hit
GAATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT17370.13501612498280233No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT17270.13423883007789272No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT17100.13291742873954634No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCC16410.1275540938956699No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT14340.11146408936404063No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGT207.856832E-444.029
TACGACT207.856832E-444.037
ACGTAAG551.8189894E-1244.01
CGTTTTT216100.043.317911
CGGTCTA1800.041.55555331
CGTAATC650.040.61538322
TCACGAC1800.040.33333225
CGACGGT1800.040.33333228
TAGCCGT557.8216544E-1140.044
TCGCGCA1650.040.034
GTTTTTT242900.039.2902452
CGGGCTA452.3512257E-839.1111076
GCGTAAG452.3512257E-839.1111071
GCGCGAC4100.038.6341489
ATCACGG404.1250496E-738.52
TGGTACG404.1250496E-738.51
ACCCGCT3900.038.3589834
GACGGTC1900.038.21052629
CGTTAGG1850.038.0540542
CTAAGCG357.290886E-637.7142871