##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546112_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1286513
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.954033888503265	31.0	28.0	33.0	23.0	33.0
2	28.944089954784754	31.0	28.0	33.0	16.0	33.0
3	28.40844126720834	31.0	28.0	33.0	16.0	34.0
4	32.11824754199919	35.0	32.0	35.0	25.0	37.0
5	33.76206692042754	35.0	33.0	35.0	30.0	37.0
6	33.676186715563695	35.0	33.0	37.0	28.0	37.0
7	34.273706522981115	35.0	33.0	37.0	31.0	37.0
8	34.72004013950889	35.0	35.0	37.0	32.0	37.0
9	36.651980197635005	39.0	37.0	39.0	32.0	39.0
10	36.27307613681323	38.0	35.0	39.0	31.0	39.0
11	36.49085940056572	39.0	35.0	39.0	32.0	39.0
12	35.3982711406725	37.0	35.0	39.0	30.0	39.0
13	35.69783826513996	37.0	35.0	39.0	30.0	39.0
14	36.47029684115124	38.0	35.0	40.0	30.0	41.0
15	36.98849914458696	39.0	35.0	40.0	32.0	41.0
16	36.66677522885505	38.0	35.0	40.0	31.0	41.0
17	36.22787954727236	38.0	34.0	40.0	31.0	41.0
18	35.83516528787506	38.0	34.0	39.0	29.0	40.0
19	35.283301451287315	37.0	34.0	39.0	29.0	41.0
20	35.38905086851046	35.0	34.0	39.0	30.0	41.0
21	35.531063424932356	36.0	34.0	39.0	30.0	40.0
22	35.90103792188653	36.0	35.0	40.0	31.0	41.0
23	36.25058044497024	37.0	35.0	40.0	32.0	41.0
24	35.82897957502178	36.0	35.0	40.0	31.0	41.0
25	35.494199436772114	35.0	34.0	40.0	30.0	41.0
26	35.5867838101908	36.0	34.0	40.0	30.0	41.0
27	35.9655961502138	37.0	35.0	40.0	31.0	41.0
28	35.86001462869011	36.0	35.0	40.0	31.0	41.0
29	35.93858437497328	37.0	35.0	40.0	31.0	41.0
30	35.586071030763	36.0	35.0	40.0	30.0	41.0
31	34.860310778048884	36.0	34.0	40.0	28.0	41.0
32	34.71316030230553	36.0	34.0	40.0	25.0	41.0
33	34.24908104309867	36.0	34.0	40.0	22.0	41.0
34	33.67712724239864	36.0	33.0	40.0	18.0	41.0
35	33.30923822767434	36.0	33.0	40.0	15.0	41.0
36	32.86629672611159	36.0	33.0	40.0	12.0	41.0
37	32.76829538450058	36.0	33.0	40.0	10.0	41.0
38	32.63374641375563	36.0	32.0	40.0	10.0	41.0
39	32.428179894023614	36.0	32.0	40.0	10.0	41.0
40	32.24208927542901	35.0	31.0	40.0	10.0	40.0
41	31.942826850564277	35.0	31.0	39.0	10.0	40.0
42	32.116768349795144	35.0	31.0	40.0	10.0	40.0
43	31.929888776872055	35.0	31.0	39.0	10.0	40.0
44	31.675342573296966	35.0	31.0	39.0	10.0	40.0
45	31.622800546904696	35.0	30.0	39.0	10.0	40.0
46	31.473969559576933	35.0	30.0	39.0	10.0	40.0
47	31.44851237414624	35.0	30.0	39.0	10.0	40.0
48	31.44881629645406	35.0	30.0	39.0	10.0	40.0
49	31.474250163037606	35.0	30.0	39.0	10.0	40.0
50	31.678670950079788	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	2.0
12	10.0
13	25.0
14	49.0
15	141.0
16	357.0
17	788.0
18	1514.0
19	2673.0
20	4278.0
21	6314.0
22	9029.0
23	13282.0
24	19676.0
25	30617.0
26	42460.0
27	47336.0
28	43148.0
29	39497.0
30	40136.0
31	46709.0
32	60566.0
33	89488.0
34	139361.0
35	114218.0
36	120245.0
37	173862.0
38	200097.0
39	40632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.764497521595196	20.884514964092862	23.86209855633017	26.48888895798177
2	44.304954555453385	18.846369993929326	25.09815291411746	11.750522536499826
3	28.010676922813836	17.769272444196055	41.70948913846965	12.51056149452046
4	24.285335632053464	22.77419660741866	39.08479743306131	13.855670327466571
5	19.871544244014636	26.841936303791726	39.2628756957761	14.023643756417542
6	17.93778997958046	34.83905720346394	38.03335061519005	9.189802201765547
7	71.04630889854981	4.63578681288102	21.961923431788097	2.355980856781082
8	71.75209267220775	3.9579079263093333	21.51653345127488	2.773465950208043
9	68.15959108069643	5.118331489848917	22.816325991264762	3.905751438189898
10	36.38750638353441	24.877712079085093	28.05428316697927	10.680498370401232
11	32.10018087652437	20.115692573646747	34.86494112379743	12.91918542603145
12	29.000872902178216	19.743990150118965	38.156551857618226	13.098585090084594
13	19.33420027625061	28.780665255617315	38.93143714832264	12.953697319809438
14	14.47408615381267	32.354978146353744	39.708265676289315	13.462670023544263
15	13.830485972547498	23.08589186428742	49.8877197509858	13.195902412179278
16	16.673908464197407	18.65360085751174	49.09340208765865	15.579088590632198
17	16.281374537218046	18.51664149526666	40.0369836915756	25.165000275939693
18	18.718660441052677	21.590531926222276	42.56653450062301	17.124273132102047
19	22.316058990464924	22.93532984120642	38.03886940901491	16.709741759313744
20	23.33221661965328	21.553144041296125	37.73828946928636	17.376349869764237
21	20.092529185480444	23.523897543203994	39.98778092409482	16.395792347220745
22	18.541825850185734	20.615881844956093	38.968047738343884	21.874244566514292
23	16.48860135886695	25.497138388807578	38.53874776236229	19.47551248996318
24	16.741610850415036	22.99160599232188	44.64090141335533	15.625881743907758
25	17.622208248187153	21.984464984030478	42.462765630817564	17.930561136964805
26	15.124448800750557	26.35814795497597	39.12653816945495	19.39086507481852
27	15.734392112633142	26.569261251149424	40.804173762721405	16.89217287349603
28	14.077432563837286	24.299016022379874	45.26102728849223	16.362524125290612
29	15.092657439139753	23.67710236896168	43.53442211621647	17.695818075682094
30	17.13935265325729	24.488365061215863	42.12122224960028	16.25106003592657
31	23.531903680724565	22.903227561633656	36.613310553410656	16.951558204231127
32	23.228214561376372	24.171228740012733	36.89725638217414	15.703300316436755
33	21.845795572994597	24.016391594954733	35.337380967001494	18.800431865049166
34	17.55411721451707	25.189485065444345	36.15462883002348	21.1017688900151
35	17.879648320693224	25.44109542616359	36.004999560828374	20.674256692314806
36	25.006509844828617	25.018169268402264	32.556608444687306	17.418712442081812
37	18.485161051617823	29.33409922791297	33.05944051867334	19.121299201795864
38	19.288573065332415	30.812047760108136	30.722192469100584	19.177186705458865
39	19.379050192263893	26.92829376772718	32.18716017638376	21.505495863625164
40	20.971027887009303	24.47289689260816	33.83743498899739	20.718640231385148
41	17.520382615643992	24.045073776945898	34.24473751917004	24.189806088240072
42	20.173601044062515	25.560876571010166	31.21826207741391	23.047260307513408
43	19.852034141901402	24.788089976549013	31.849736458162493	23.510139423387095
44	18.71982638341004	27.02996394128936	33.359864999420914	20.890344675879685
45	18.45515746828831	30.363315411503812	30.41174088407968	20.7697862361282
46	20.81494707010345	27.79987454460235	31.807529344825898	19.5776490404683
47	19.934505131312314	26.039534773453514	32.55007916748606	21.475880927748108
48	19.822963312457784	26.450101942226777	33.624145267090185	20.10278947822525
49	19.915383676651537	24.862399369458373	34.75985085265364	20.462366101236444
50	17.853064834945314	28.045499734553793	33.22220607176142	20.879229358739476
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	614.0
1	1296.0
2	1978.0
3	29483.5
4	56989.0
5	38984.0
6	20979.0
7	20765.5
8	20552.0
9	21058.0
10	21564.0
11	21436.0
12	21308.0
13	20314.0
14	19320.0
15	17931.5
16	16543.0
17	15469.5
18	14396.0
19	13609.5
20	12823.0
21	12062.5
22	11302.0
23	11680.5
24	12059.0
25	12568.5
26	13078.0
27	14255.5
28	15433.0
29	17358.5
30	19284.0
31	21118.0
32	22952.0
33	26246.5
34	29541.0
35	33890.5
36	38240.0
37	41358.0
38	44476.0
39	51264.0
40	58052.0
41	68581.0
42	79110.0
43	93185.0
44	107260.0
45	107893.5
46	108527.0
47	106111.5
48	103696.0
49	99141.5
50	94587.0
51	85827.0
52	77067.0
53	66743.5
54	56420.0
55	52094.5
56	47769.0
57	43182.0
58	38595.0
59	34371.0
60	30147.0
61	27293.5
62	24440.0
63	20420.5
64	16401.0
65	13411.5
66	10422.0
67	8617.5
68	6813.0
69	5863.5
70	4914.0
71	4255.0
72	3596.0
73	2993.0
74	2390.0
75	1905.0
76	1420.0
77	1126.5
78	833.0
79	573.5
80	314.0
81	225.0
82	136.0
83	104.5
84	73.0
85	57.5
86	42.0
87	33.0
88	24.0
89	19.5
90	15.0
91	10.0
92	5.0
93	5.5
94	6.0
95	4.5
96	3.0
97	3.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1286513.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.18970766903483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.81807048226585	23.006764364147237
2	7.110978098764311	4.151347438876787
3	2.945665461784819	2.5794934116081403
4	1.776174570509366	2.073840659293676
5	1.2166821503860914	1.7757298147951344
6	0.9285371503794884	1.6262236787649538
7	0.7155086729083082	1.4619842297796808
8	0.5599826218375632	1.3076583224942522
9	0.4700160955327958	1.2347669185509111
>10	4.33271766715184	28.09481837637608
>50	0.8516980894833649	17.457765308144072
>100	0.2688705943937003	10.913381054067603
>500	0.0029516731909367427	0.5131912228098767
>1k	0.0018783374851415633	0.8314293086274177
>5k	0.0	0.0
>10k+	2.683339264487948E-4	2.971605891664211
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37939	2.948979139736637	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	1737	0.13501612498280233	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1727	0.13423883007789272	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1710	0.13291742873954634	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCC	1641	0.1275540938956699	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1434	0.11146408936404063	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.772949049096277E-5	0.0	0.0	0.04391716212739397	0.0
2	7.772949049096277E-5	0.0	0.0	0.17745642679086804	0.0
3	1.5545898098192555E-4	0.0	0.0	0.238085429373819	0.0
4	1.5545898098192555E-4	0.0	0.0	0.38118542136768147	0.0
5	2.3318847147288835E-4	0.0	0.0	0.7369532993448181	0.0
6	2.3318847147288835E-4	0.0	0.0	0.9681208040649414	0.0
7	2.3318847147288835E-4	0.0	0.0	1.1392034126355506	0.0
8	2.3318847147288835E-4	0.0	0.0	1.4882088249399734	0.0
9	2.3318847147288835E-4	0.0	0.0	1.627189153937815	0.0
10	2.3318847147288835E-4	0.0	0.0	1.9939168900741773	0.0
11	2.3318847147288835E-4	0.0	0.0	2.2170005277832403	0.0
12	2.3318847147288835E-4	0.0	0.0	2.5098852479531883	0.0
13	2.3318847147288835E-4	0.0	0.0	2.606425275142964	0.0
14	2.3318847147288835E-4	0.0	0.0	2.6492542244034842	0.0
15	2.3318847147288835E-4	0.0	0.0	2.713225595077547	0.0
16	2.3318847147288835E-4	0.0	0.0	2.8254669793464973	0.0
17	2.3318847147288835E-4	0.0	0.0	2.953098802732658	0.0
18	3.109179619638511E-4	0.0	0.0	3.160325624381565	0.0
19	3.886474524548139E-4	0.0	0.0	3.266348649411238	0.0
20	3.886474524548139E-4	0.0	0.0	3.3627332176200317	0.0
21	3.886474524548139E-4	0.0	0.0	3.4726427171742533	0.0
22	3.886474524548139E-4	0.0	0.0	3.58620550278155	0.0
23	3.886474524548139E-4	0.0	0.0	3.7160137519014578	0.0
24	3.886474524548139E-4	0.0	0.0	3.8144192868630165	0.0
25	3.886474524548139E-4	0.0	0.0	3.8955688749355817	0.0
26	4.663769429457767E-4	0.0	0.0	3.982081797852023	0.0
27	4.663769429457767E-4	0.0	0.0	4.077611341665417	0.0
28	4.663769429457767E-4	0.0	0.0	4.175084122741084	0.0
29	4.663769429457767E-4	0.0	0.0	4.283283573504504	0.0
30	4.663769429457767E-4	0.0	0.0	4.410604478928701	0.0
31	4.663769429457767E-4	0.0	0.0	4.523001322178633	0.0
32	4.663769429457767E-4	0.0	0.0	4.6315894203945085	0.0
33	4.663769429457767E-4	0.0	0.0	4.743053509758549	0.0
34	4.663769429457767E-4	0.0	0.0	4.862990113586105	0.0
35	4.663769429457767E-4	0.0	0.0	5.014873538005446	0.0
36	4.663769429457767E-4	0.0	0.0	5.12423893112623	0.0
37	4.663769429457767E-4	0.0	0.0	5.239123118071873	0.0
38	4.663769429457767E-4	0.0	0.0	5.38012441382248	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGT	20	7.856832E-4	44.0	29
TACGACT	20	7.856832E-4	44.0	37
ACGTAAG	55	1.8189894E-12	44.0	1
CGTTTTT	21610	0.0	43.31791	1
CGGTCTA	180	0.0	41.555553	31
CGTAATC	65	0.0	40.615383	22
TCACGAC	180	0.0	40.333332	25
CGACGGT	180	0.0	40.333332	28
TAGCCGT	55	7.8216544E-11	40.0	44
TCGCGCA	165	0.0	40.0	34
GTTTTTT	24290	0.0	39.290245	2
CGGGCTA	45	2.3512257E-8	39.111107	6
GCGTAAG	45	2.3512257E-8	39.111107	1
GCGCGAC	410	0.0	38.634148	9
ATCACGG	40	4.1250496E-7	38.5	2
TGGTACG	40	4.1250496E-7	38.5	1
ACCCGCT	390	0.0	38.35898	34
GACGGTC	190	0.0	38.210526	29
CGTTAGG	185	0.0	38.054054	2
CTAAGCG	35	7.290886E-6	37.714287	1
>>END_MODULE