FastQCFastQC Report
Thu 26 May 2016
SRR1546111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546111_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1268641
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377142.9727874158252807No Hit
GAATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT17600.13873113039859186No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT17520.13810053435132555No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCC16380.1291145406777804No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT16080.12674980550053166No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT14430.11374376202566369No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGC207.8567903E-444.00000436
CGACAGT207.8567903E-444.00000444
CCGTATG207.8567903E-444.0000041
CGCTACG207.8567903E-444.00000412
TGTTACG207.8567903E-444.0000041
TCGTAAG207.8567903E-444.0000041
ACCCGTA207.8567903E-444.00000437
ACGTAAG650.044.0000041
GTACGAC302.5279242E-644.00000426
TTCGTAC454.802132E-1044.033
TCGTACA454.802132E-1044.034
CGTTTTT217400.043.3118671
TCACGAC1200.042.1666725
CGACGGT1200.042.1666728
CGGTCTA1200.042.1666731
TCGTAAT750.041.06666621
TCGCTAT700.040.85714323
CGTAATC700.040.85714322
CGTACAT501.3478711E-939.635
AACACGT1000.039.641