##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546111_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1268641
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.850432864774195	31.0	28.0	33.0	16.0	33.0
2	28.859226526653323	31.0	28.0	33.0	16.0	33.0
3	28.297400919566687	31.0	28.0	33.0	16.0	34.0
4	32.02472330627813	35.0	32.0	35.0	25.0	37.0
5	33.7065678943058	35.0	33.0	35.0	30.0	37.0
6	33.621248249110664	35.0	33.0	37.0	28.0	37.0
7	34.19115179156278	35.0	33.0	37.0	30.0	37.0
8	34.701112450251884	35.0	35.0	37.0	32.0	37.0
9	36.64117035473392	39.0	37.0	39.0	32.0	39.0
10	36.348189125213516	39.0	35.0	39.0	32.0	39.0
11	36.50638675559122	39.0	35.0	39.0	32.0	39.0
12	35.56932970004911	37.0	35.0	39.0	30.0	39.0
13	35.77043623846305	37.0	35.0	39.0	30.0	39.0
14	36.53576464894324	38.0	35.0	40.0	31.0	41.0
15	36.96479066970088	39.0	35.0	40.0	32.0	41.0
16	36.635181268775014	38.0	35.0	40.0	31.0	41.0
17	36.18968802048806	38.0	34.0	40.0	30.0	41.0
18	35.780781954863514	38.0	34.0	39.0	29.0	40.0
19	35.20390717310886	37.0	34.0	39.0	28.0	41.0
20	35.47891089756676	35.0	34.0	39.0	30.0	41.0
21	35.630459680871105	36.0	34.0	39.0	31.0	41.0
22	35.926943083189016	36.0	35.0	40.0	31.0	41.0
23	36.24772413945316	37.0	35.0	40.0	32.0	41.0
24	35.85152300769091	36.0	35.0	40.0	31.0	41.0
25	35.5284544642653	36.0	34.0	40.0	30.0	41.0
26	35.605980730561285	36.0	34.0	40.0	30.0	41.0
27	35.96851118637976	37.0	35.0	40.0	31.0	41.0
28	35.8731918643651	36.0	35.0	40.0	31.0	41.0
29	35.94586411758724	37.0	35.0	40.0	31.0	41.0
30	35.5923220201775	36.0	35.0	40.0	30.0	41.0
31	34.84985271641071	36.0	34.0	40.0	28.0	41.0
32	34.71346503857277	36.0	34.0	40.0	25.0	41.0
33	34.26657580828619	36.0	34.0	40.0	22.0	41.0
34	33.687349691520296	36.0	33.0	40.0	18.0	41.0
35	33.282890116274025	36.0	33.0	40.0	15.0	41.0
36	32.885816397231366	36.0	33.0	40.0	12.0	41.0
37	32.80683108933103	36.0	33.0	40.0	10.0	41.0
38	32.66218654449919	36.0	32.0	40.0	10.0	41.0
39	32.43155707564236	36.0	32.0	40.0	10.0	41.0
40	32.29432045787579	35.0	31.0	40.0	10.0	41.0
41	31.983330193490513	35.0	31.0	39.0	10.0	40.0
42	32.17546177366174	35.0	31.0	40.0	10.0	41.0
43	31.987641105718637	35.0	31.0	40.0	10.0	40.0
44	31.753042034744265	35.0	31.0	39.0	10.0	40.0
45	31.730018184813513	35.0	31.0	39.0	10.0	40.0
46	31.572798766554133	35.0	30.0	39.0	10.0	40.0
47	31.555932687024935	35.0	30.0	39.0	10.0	40.0
48	31.526621006257876	35.0	31.0	39.0	10.0	40.0
49	31.574603847739432	35.0	31.0	39.0	10.0	40.0
50	31.70295300246484	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	5.0
12	9.0
13	23.0
14	55.0
15	150.0
16	320.0
17	787.0
18	1470.0
19	2525.0
20	4223.0
21	6235.0
22	9084.0
23	13013.0
24	19539.0
25	29877.0
26	42341.0
27	46651.0
28	42089.0
29	39420.0
30	39795.0
31	46119.0
32	59486.0
33	88050.0
34	136788.0
35	110537.0
36	116605.0
37	168801.0
38	198734.0
39	45904.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.76156454032307	20.88865171470889	23.841417706033464	26.50836603893458
2	44.37346735601324	18.81682840141537	25.110255777639225	11.699448464932159
3	27.983172544478695	17.866362509173204	41.69950364208629	12.450961304261805
4	24.24097912648259	22.810077870729387	39.070154598503436	13.878788404284585
5	19.85092709442624	26.829102953475413	39.263984058531925	14.055985893566422
6	17.94936471389463	34.779421443891536	38.04322893553022	9.22798490668361
7	71.14226956246881	4.593655730817465	21.914946781634836	2.349127925078884
8	71.80912488245296	3.9401217523318257	21.50111812561631	2.749635239598909
9	68.1672750604781	5.143693133045519	22.78406578377965	3.9049660226967284
10	36.28402361266899	25.26222942503041	28.04189680138037	10.41185016092023
11	32.07834209993214	20.213125699074837	34.855487092093036	12.853045108899996
12	29.004186369508787	20.019848010587708	38.21656402402256	12.759401595880945
13	19.31507810326168	28.840231397219547	38.937965902095236	12.90672459742354
14	14.555339138495446	32.36439623187332	39.62917799440503	13.451086635226197
15	13.848835092039435	23.132943046929746	49.77491662337887	13.243305237651946
16	16.72190950789073	18.636004984861753	49.11507668442057	15.527008822826946
17	16.214752636876785	18.519581189635208	40.02944883540734	25.236217338080667
18	18.690709191962107	21.59152983389312	42.595501800745836	17.122259173398938
19	22.34643212697682	22.91365327149288	38.05394906833375	16.685965533196548
20	23.382422608129485	21.759031908948238	37.746139372761874	17.1124061101604
21	20.279653582061435	23.444378669773403	39.85839965758635	16.417568090578815
22	18.487893738260077	20.661479488681195	39.000552559786414	21.850074213272315
23	16.50230443443023	25.457477726165244	38.527132577301224	19.513085262103306
24	16.737201462036936	23.103226208202322	44.65211198439905	15.50746034536169
25	17.633436094214204	22.01450213259701	42.55293656755536	17.79912520563343
26	15.19066465611627	26.40888951247831	39.07827352261199	19.322172308793427
27	15.833242028280656	26.59396945235098	40.77906988659518	16.79371863277318
28	14.045344585268804	24.33958858337386	45.21200244986564	16.403064381491692
29	15.021191968413444	23.70150420804625	43.656243176753705	17.621060646786603
30	17.084108112539322	24.535940427591417	42.12878190126285	16.251169558606414
31	23.538889252357446	22.856190206685735	36.60397228215074	17.000948258806076
32	23.23888318287049	24.184067833216805	36.94796242593452	15.629086557978184
33	21.85969080299312	24.030517695707452	35.40315975914384	18.706631742155583
34	17.569588244428484	25.178438975249897	36.085701155803726	21.16627162451789
35	17.893556963711564	25.437298652652718	35.96423259219905	20.704911791436665
36	25.028987712047773	25.048536189513033	32.61639817726213	17.30607792117707
37	18.530380146944644	29.356847208942483	33.17959927197686	18.93317337213601
38	19.240667769684254	30.875953086807062	30.827397191167556	19.055981952341128
39	19.40596275857394	27.005275724180443	32.25088894336538	21.33787257388024
40	21.011696768431733	24.57771741572281	33.801524623593274	20.60906119225218
41	17.474683539314906	24.160026358914777	34.2913401033074	24.073949998462922
42	20.227787057173778	25.594553541939764	31.160903675665537	23.016755725220925
43	19.950324796376595	24.747111278919725	31.791578547437773	23.510985377265907
44	18.692916278127537	27.111609982650727	33.40164790512052	20.793825834101217
45	18.44327906791598	30.52100633670203	30.374786878242148	20.660927717139838
46	20.89085880087432	27.873527656760267	31.974530225650916	19.2610833167145
47	19.935190491242203	26.102498658012784	32.56500459940992	21.397306251335092
48	19.872446184539204	26.470530276098597	33.61321287897837	20.04381066038383
49	19.821367904710634	24.91816045674072	34.924300885750974	20.336170752797678
50	17.9024641328792	28.035354367389985	33.24943778421161	20.812743715519204
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	656.0
1	1311.5
2	1967.0
3	29559.0
4	57151.0
5	38956.5
6	20762.0
7	20470.5
8	20179.0
9	20725.5
10	21272.0
11	21104.0
12	20936.0
13	19856.5
14	18777.0
15	17486.5
16	16196.0
17	15159.5
18	14123.0
19	13457.0
20	12791.0
21	11906.0
22	11021.0
23	11389.0
24	11757.0
25	12393.0
26	13029.0
27	14125.5
28	15222.0
29	17142.0
30	19062.0
31	20913.0
32	22764.0
33	26012.5
34	29261.0
35	33522.5
36	37784.0
37	41213.0
38	44642.0
39	51041.5
40	57441.0
41	67976.5
42	78512.0
43	92133.5
44	105755.0
45	106402.5
46	107050.0
47	104662.5
48	102275.0
49	97875.5
50	93476.0
51	84261.5
52	75047.0
53	64967.5
54	54888.0
55	50725.5
56	46563.0
57	42281.5
58	38000.0
59	33908.0
60	29816.0
61	26889.0
62	23962.0
63	19941.0
64	15920.0
65	13072.5
66	10225.0
67	8541.0
68	6857.0
69	5793.0
70	4729.0
71	4147.5
72	3566.0
73	3015.0
74	2464.0
75	1932.5
76	1401.0
77	1100.5
78	800.0
79	532.0
80	264.0
81	199.0
82	134.0
83	99.5
84	65.0
85	48.5
86	32.0
87	27.0
88	22.0
89	14.5
90	7.0
91	7.0
92	7.0
93	5.5
94	4.0
95	3.0
96	2.0
97	3.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1268641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.7780905562463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.58256720174491	22.614562350741256
2	7.064859317149137	4.066263223921165
3	3.029789405443563	2.6157466162863146
4	1.8177974543953879	2.092509590220179
5	1.2176203654781492	1.752039457042995
6	0.9357476711981351	1.6157418731721913
7	0.7481198955552611	1.507062347085318
8	0.5526628103248954	1.272366432207953
9	0.45920599547036345	1.189356454945561
>10	4.424356544499015	28.197426644660766
>50	0.8870406383445275	17.787118658903587
>100	0.2758195870053224	11.066547618235969
>500	0.0022065566956434167	0.39767180863454554
>1k	0.0019307371086879897	0.8320151493228157
>5k	0.0	0.0
>10k+	2.758195869554271E-4	2.9935717746194115
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37714	2.9727874158252807	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	1760	0.13873113039859186	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1752	0.13810053435132555	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCC	1638	0.1291145406777804	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1608	0.12674980550053166	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1443	0.11374376202566369	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03941225295414542	0.0
2	0.0	0.0	0.0	0.18184813513042697	0.0
3	0.0	0.0	0.0	0.23852295487848807	0.0
4	0.0	0.0	0.0	0.384032992785193	0.0
5	0.0	0.0	0.0	0.7444186337978987	0.0
6	0.0	0.0	0.0	0.9627625151638644	0.0
7	0.0	0.0	0.0	1.123406858204961	0.0
8	0.0	0.0	0.0	1.4577804122679308	0.0
9	0.0	0.0	0.0	1.5953291750778984	0.0
10	0.0	0.0	0.0	1.9366392856607977	0.0
11	0.0	0.0	0.0	2.1520666603081566	0.0
12	7.882450590829084E-5	0.0	0.0	2.432603076835764	0.0
13	7.882450590829084E-5	0.0	0.0	2.5266407123843546	0.0
14	7.882450590829084E-5	0.0	0.0	2.564949422255784	0.0
15	7.882450590829084E-5	0.0	0.0	2.629821990618307	0.0
16	7.882450590829084E-5	0.0	0.0	2.7425410340671634	0.0
17	7.882450590829084E-5	0.0	0.0	2.872128521780393	0.0
18	7.882450590829084E-5	0.0	0.0	3.0705298031515613	0.0
19	7.882450590829084E-5	0.0	0.0	3.1658286307946852	0.0
20	1.5764901181658167E-4	0.0	0.0	3.2614427564614417	0.0
21	1.5764901181658167E-4	0.0	0.0	3.3691958560380755	0.0
22	1.5764901181658167E-4	0.0	0.0	3.478525445732875	0.0
23	1.5764901181658167E-4	0.0	0.0	3.5953433634889618	0.0
24	1.5764901181658167E-4	0.0	0.0	3.6934010488388758	0.0
25	1.5764901181658167E-4	0.0	0.0	3.7679690314281187	0.0
26	1.5764901181658167E-4	0.0	0.0	3.8523900772558983	0.0
27	1.5764901181658167E-4	0.0	0.0	3.943195908062249	0.0
28	1.5764901181658167E-4	0.0	0.0	4.037312368116749	0.0
29	1.5764901181658167E-4	0.0	0.0	4.143646626587033	0.0
30	1.5764901181658167E-4	0.0	0.0	4.273549412323896	0.0
31	1.5764901181658167E-4	0.0	0.0	4.381460160912346	0.0
32	1.5764901181658167E-4	0.0	0.0	4.484089667604941	0.0
33	2.3647351772487253E-4	0.0	0.0	4.592867485758382	0.0
34	2.3647351772487253E-4	0.0	0.0	4.710394824067644	0.0
35	2.3647351772487253E-4	0.0	0.0	4.861343752882021	0.0
36	3.1529802363316334E-4	0.0	0.0	4.973116902259977	0.0
37	3.1529802363316334E-4	0.0	0.0	5.082210018437052	0.0
38	3.941225295414542E-4	0.0	0.0	5.224724725119242	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGC	20	7.8567903E-4	44.000004	36
CGACAGT	20	7.8567903E-4	44.000004	44
CCGTATG	20	7.8567903E-4	44.000004	1
CGCTACG	20	7.8567903E-4	44.000004	12
TGTTACG	20	7.8567903E-4	44.000004	1
TCGTAAG	20	7.8567903E-4	44.000004	1
ACCCGTA	20	7.8567903E-4	44.000004	37
ACGTAAG	65	0.0	44.000004	1
GTACGAC	30	2.5279242E-6	44.000004	26
TTCGTAC	45	4.802132E-10	44.0	33
TCGTACA	45	4.802132E-10	44.0	34
CGTTTTT	21740	0.0	43.311867	1
TCACGAC	120	0.0	42.16667	25
CGACGGT	120	0.0	42.16667	28
CGGTCTA	120	0.0	42.16667	31
TCGTAAT	75	0.0	41.066666	21
TCGCTAT	70	0.0	40.857143	23
CGTAATC	70	0.0	40.857143	22
CGTACAT	50	1.3478711E-9	39.6	35
AACACGT	100	0.0	39.6	41
>>END_MODULE