FastQCFastQC Report
Thu 26 May 2016
SRR1546110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546110_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1130781
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195171.725975233046894No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT11430.10108058058987548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTA207.8564254E-444.00000413
GTACGTC207.8564254E-444.00000440
CGAACTA600.044.035
ATCACGC302.5276804E-644.026
ACGGTAG302.5276804E-644.01
CGTTTTT199950.043.59291
TTAGATC1500.041.06666622
CGTAATC700.040.8571422
GTCGAAC650.040.61538733
CGTTAGG1650.040.02
AGCGTAT501.3478711E-939.630
GTTTTTT226900.039.006612
CGCATGG850.038.823532
ACGGGAT3400.038.823535
TCGTAAT850.038.8235321
CGACGGT1250.038.7228
AGGCGAA1250.038.726
CGGTCTA1250.038.7231
GTTTACG404.1244675E-738.5000041
GCGGAAT3400.038.1764688
Produced by FastQC (version 0.11.5)