##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546110_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1130781
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.831044207499065	28.0	25.0	30.0	16.0	33.0
2	26.260311236216385	30.0	25.0	31.0	16.0	33.0
3	26.585819889085506	30.0	25.0	31.0	16.0	33.0
4	27.8256046042514	32.0	19.0	35.0	10.0	35.0
5	31.30914562589927	33.0	30.0	35.0	19.0	37.0
6	30.047713925154383	33.0	28.0	35.0	17.0	37.0
7	30.129713888011914	32.0	28.0	35.0	17.0	35.0
8	32.44392238638605	35.0	32.0	35.0	28.0	35.0
9	27.310929348830587	31.0	23.0	34.0	10.0	37.0
10	31.64151502368717	34.0	30.0	37.0	17.0	39.0
11	33.872366090339334	35.0	32.0	38.0	27.0	39.0
12	33.523041154741726	35.0	32.0	39.0	25.0	39.0
13	33.766266854501445	35.0	32.0	39.0	25.0	39.0
14	33.543014076111994	36.0	31.0	39.0	22.0	40.0
15	32.25469034233861	35.0	28.0	38.0	18.0	40.0
16	30.88319223616244	34.0	27.0	38.0	17.0	40.0
17	31.177651552334183	33.0	27.0	38.0	17.0	40.0
18	32.06817058298645	34.0	29.0	38.0	18.0	39.0
19	31.811907876060882	35.0	29.0	37.0	18.0	39.0
20	32.190390535391025	34.0	30.0	37.0	22.0	39.0
21	33.04134487579823	35.0	31.0	37.0	25.0	39.0
22	33.34770923812834	35.0	31.0	37.0	26.0	39.0
23	34.50283741944727	35.0	33.0	38.0	29.0	40.0
24	34.12304681454676	35.0	33.0	38.0	27.0	40.0
25	32.9572348668752	35.0	31.0	38.0	23.0	40.0
26	26.452976305756817	33.0	20.0	36.0	0.0	39.0
27	29.840794106020528	34.0	24.0	37.0	15.0	39.0
28	31.33284429080432	34.0	28.0	37.0	20.0	39.0
29	32.54322455011182	35.0	30.0	37.0	23.0	39.0
30	32.38352961360334	35.0	31.0	38.0	22.0	39.0
31	31.227098792781273	34.0	29.0	37.0	19.0	39.0
32	31.45334330873971	34.0	30.0	37.0	18.0	39.0
33	31.063375666906325	34.0	29.0	37.0	16.0	39.0
34	30.797036738325104	34.0	29.0	37.0	15.0	39.0
35	29.834069550160464	34.0	26.0	37.0	10.0	39.0
36	29.577736980016468	34.0	25.0	37.0	9.0	39.0
37	29.68912017446349	34.0	26.0	37.0	9.0	39.0
38	29.42302178759636	33.0	25.0	37.0	9.0	39.0
39	29.35531725418096	34.0	25.0	37.0	8.0	39.0
40	29.276906845799495	33.0	25.0	37.0	9.0	39.0
41	28.6616037941918	33.0	23.0	36.0	8.0	38.0
42	28.829552318264987	33.0	24.0	36.0	8.0	38.0
43	28.433868273343823	33.0	23.0	36.0	8.0	38.0
44	28.204056311522745	33.0	23.0	36.0	8.0	38.0
45	27.67071696464656	32.0	23.0	35.0	8.0	38.0
46	27.261555508980077	31.0	22.0	35.0	7.0	38.0
47	27.264965541515114	31.0	23.0	35.0	7.0	38.0
48	27.289409708864934	31.0	23.0	35.0	7.0	38.0
49	27.10669174667774	31.0	22.0	35.0	7.0	38.0
50	28.956278890430596	34.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	7.0
11	19.0
12	55.0
13	136.0
14	307.0
15	760.0
16	1660.0
17	3024.0
18	5132.0
19	8392.0
20	12159.0
21	17598.0
22	23765.0
23	31248.0
24	40666.0
25	50483.0
26	58007.0
27	61709.0
28	66598.0
29	75116.0
30	89588.0
31	104865.0
32	117257.0
33	119364.0
34	108177.0
35	83720.0
36	42140.0
37	8569.0
38	259.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.94783516879042	21.804222037688994	23.687610598338672	27.560332195181914
2	44.32069516555372	16.34206800432621	26.5911790169803	12.74605781313977
3	29.286484297136226	14.011731714629091	43.124265441318876	13.577518546915805
4	25.44931335068417	22.735525269702975	37.89000699516529	13.925154384447563
5	19.173916081009498	27.15627517618354	39.78285804236187	13.886950700445091
6	17.94388126436507	33.52399801553086	39.016485066515976	9.515635653588095
7	72.78243974739583	3.519779692088919	21.961635365291777	1.7361451952234783
8	74.087113242971	2.7744541162258654	21.149718645785523	1.988713995017603
9	62.50874395661052	3.5345482458583932	20.82295333932919	13.1337544582019
10	36.786433447325344	25.48795920695519	28.052469930074874	9.673137415644586
11	32.68944207587499	19.80171226789272	35.271020648560594	12.23782500767169
12	29.16957394933236	20.068165276919224	38.68025727351273	12.082003500235677
13	18.84891946362735	29.40312934157896	39.21731971089009	12.530631483903603
14	13.911004871854054	32.621347546518734	40.278267852042084	13.189379729585127
15	12.54522316876566	21.609843108435676	49.50967517140808	16.335258551390588
16	15.021387872629624	16.86639588036941	48.85809011647702	19.254126130523947
17	15.496723061317796	17.833957238404253	40.71707961134826	25.952240088929685
18	18.159572896962363	21.326941291019217	43.437146538542834	17.07633927347559
19	22.568118848831027	22.419902704414028	38.829534631374244	16.182443815380697
20	23.473952958176696	21.36779800863297	38.347832162018996	16.81041687117134
21	19.949839977856012	23.185126032361705	40.482816743471986	16.382217246310294
22	18.189286873408733	20.370876411966595	39.26896543185639	22.17087128276828
23	15.879732680333328	25.45736088597173	39.20582323190786	19.457083201787086
24	15.998500151665088	22.608533394176238	46.22398147828802	15.168984975870659
25	17.169372318777906	21.060134544177874	43.859597923912766	17.910895213131457
26	12.006568911221537	38.646033139927184	32.520266965928855	16.827130982922423
27	15.09160482887491	27.297504998757493	41.41491588556935	16.19597428679824
28	13.592994576314954	24.08450442658658	46.392449112604474	15.930051884493993
29	14.413312568923603	23.543550873246012	44.5034891813711	17.539647376459282
30	16.74745154013023	24.340522170075374	42.779105768490986	16.13292052130342
31	24.056824442575532	22.568649455553285	36.988594608505096	16.38593149336609
32	23.271880231450652	23.660372786596167	37.34268616115764	15.72506082079554
33	21.82809934019054	23.524537465698486	35.882633330415	18.764729863695976
34	17.269126382562142	24.727599773961536	36.23168411920611	21.771589724270214
35	17.714659160350234	24.88598588055512	36.01227823955302	21.387076719541625
36	25.15960208033209	24.39959638515327	32.501961034011	17.938840500503634
37	17.941493534114915	29.511638416280427	32.705094974181556	19.84177307542309
38	18.56814007309992	31.280327490468977	30.727612154785056	19.423920281646048
39	18.92170101903021	26.860904100794052	32.07402671251109	22.143368167664647
40	20.619465661343796	24.160558056776686	33.890558826156436	21.32941745572308
41	16.926973481160363	23.51763957830915	34.29496958296965	25.26041735756084
42	19.53172188071784	25.158717735794994	30.298262882025785	25.011297501461378
43	19.438688835415523	24.219366968493457	31.764594558981802	24.57734963710922
44	18.123933812117464	26.564206508598925	33.501270360927535	21.810589318356076
45	17.971826551737248	31.244334667809238	29.640664284242487	21.143174496211024
46	20.17738182725037	27.443333412924343	31.521222942373456	20.85806181745183
47	19.53189874962526	25.497863865770647	31.94261311429888	23.027624270305214
48	19.226711449874028	26.075694586308046	33.84846402619075	20.849129937627183
49	19.536497341218148	24.760320521834025	34.09183564279909	21.611346494148734
50	17.40390049001531	28.446445421350376	33.10632209066123	21.043331997973084
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	371.0
1	787.0
2	1203.0
3	17283.0
4	33363.0
5	25954.0
6	18545.0
7	17974.5
8	17404.0
9	18217.0
10	19030.0
11	19649.0
12	20268.0
13	19909.5
14	19551.0
15	18964.0
16	18377.0
17	17439.5
18	16502.0
19	15771.0
20	15040.0
21	14193.0
22	13346.0
23	13403.5
24	13461.0
25	13418.0
26	13375.0
27	14401.5
28	15428.0
29	17060.0
30	18692.0
31	20117.5
32	21543.0
33	24239.5
34	26936.0
35	30574.5
36	34213.0
37	36864.5
38	39516.0
39	44509.0
40	49502.0
41	58995.5
42	68489.0
43	77857.5
44	87226.0
45	89757.5
46	92289.0
47	91022.0
48	89755.0
49	86061.5
50	82368.0
51	74615.5
52	66863.0
53	58558.5
54	50254.0
55	45844.5
56	41435.0
57	37776.0
58	34117.0
59	30617.0
60	27117.0
61	24375.0
62	21633.0
63	18115.0
64	14597.0
65	12040.0
66	9483.0
67	7955.5
68	6428.0
69	5533.5
70	4639.0
71	3974.5
72	3310.0
73	2821.5
74	2333.0
75	1831.5
76	1330.0
77	1043.0
78	756.0
79	556.5
80	357.0
81	253.0
82	149.0
83	120.0
84	91.0
85	69.0
86	47.0
87	33.5
88	20.0
89	15.0
90	10.0
91	10.0
92	10.0
93	7.5
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1130781.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.24444433401361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.26950029277572	36.84212770424845
2	6.816917346118272	6.032214400998033
3	2.558077617334979	3.3954216822679086
4	1.4074188809440467	2.490818653302744
5	0.9035532405232194	1.9988605526573593
6	0.634157556212239	1.6834769216895906
7	0.49156675143765804	1.5224368439304758
8	0.38860481404060443	1.3754883250199386
9	0.3255821811488478	1.2964682420988238
>10	2.7687482287629828	25.647704055967274
>50	0.37320614146698844	11.284982798405867
>100	0.06093820581691391	4.0435963119106795
>500	0.0010804646359907951	0.30910426841974575
>1k	4.321858543963181E-4	0.21129614389348533
>5k	0.0	0.0
>10k+	2.1609292719815905E-4	1.8660030951896622
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19517	1.725975233046894	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1143	0.10108058058987548	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.033605092409582406	0.0
2	0.0	0.0	0.0	0.12973334359172997	0.0
3	0.0	0.0	0.0	0.1812021956506167	0.0
4	0.0	0.0	0.0	0.2553102678591168	0.0
5	0.0	0.0	0.0	0.4638387097059466	0.0
6	0.0	0.0	0.0	0.5964019558163782	0.0
7	0.0	0.0	0.0	0.6726324549139047	0.0
8	0.0	0.0	0.0	0.8645352194633621	0.0
9	0.0	0.0	0.0	0.9687994403867769	0.0
10	0.0	0.0	0.0	1.1856407208822928	0.0
11	0.0	0.0	0.0	1.3396051047904058	0.0
12	8.843445370942738E-5	0.0	0.0	1.5069230912086424	0.0
13	8.843445370942738E-5	0.0	0.0	1.5712149390553962	0.0
14	8.843445370942738E-5	0.0	0.0	1.592704511306787	0.0
15	8.843445370942738E-5	0.0	0.0	1.6315272364852258	0.0
16	8.843445370942738E-5	0.0	0.0	1.698648986850681	0.0
17	8.843445370942738E-5	0.0	0.0	1.795042541393957	0.0
18	8.843445370942738E-5	0.0	0.0	1.9225650236429512	0.0
19	8.843445370942738E-5	0.0	0.0	1.98146236981343	0.0
20	8.843445370942738E-5	0.0	0.0	2.040890322706165	0.0
21	8.843445370942738E-5	0.0	0.0	2.1009373167748664	0.0
22	8.843445370942738E-5	0.0	0.0	2.1628414343714653	0.0
23	8.843445370942738E-5	0.0	0.0	2.2319087427185282	0.0
24	8.843445370942738E-5	0.0	0.0	2.2851462838516032	0.0
25	8.843445370942738E-5	0.0	0.0	2.3290982073451887	0.0
26	8.843445370942738E-5	0.0	0.0	2.380036452681819	0.0
27	8.843445370942738E-5	0.0	0.0	2.445389513973086	0.0
28	8.843445370942738E-5	0.0	0.0	2.5160486424869184	0.0
29	8.843445370942738E-5	0.0	0.0	2.5909526247788035	0.0
30	8.843445370942738E-5	0.0	0.0	2.685312186886762	0.0
31	8.843445370942738E-5	0.0	0.0	2.770120827994103	0.0
32	8.843445370942738E-5	0.0	0.0	2.8486506228880746	0.0
33	8.843445370942738E-5	0.0	0.0	2.934166739625091	0.0
34	8.843445370942738E-5	0.0	0.0	3.021451545436296	0.0
35	8.843445370942738E-5	0.0	0.0	3.136770073073389	0.0
36	8.843445370942738E-5	0.0	0.0	3.2148576956988135	0.0
37	8.843445370942738E-5	0.0	0.0	3.299577902352445	0.0
38	8.843445370942738E-5	0.0	0.0	3.4037536888221505	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGTA	20	7.8564254E-4	44.000004	13
GTACGTC	20	7.8564254E-4	44.000004	40
CGAACTA	60	0.0	44.0	35
ATCACGC	30	2.5276804E-6	44.0	26
ACGGTAG	30	2.5276804E-6	44.0	1
CGTTTTT	19995	0.0	43.5929	1
TTAGATC	150	0.0	41.066666	22
CGTAATC	70	0.0	40.85714	22
GTCGAAC	65	0.0	40.615387	33
CGTTAGG	165	0.0	40.0	2
AGCGTAT	50	1.3478711E-9	39.6	30
GTTTTTT	22690	0.0	39.00661	2
CGCATGG	85	0.0	38.82353	2
ACGGGAT	340	0.0	38.82353	5
TCGTAAT	85	0.0	38.82353	21
CGACGGT	125	0.0	38.72	28
AGGCGAA	125	0.0	38.72	6
CGGTCTA	125	0.0	38.72	31
GTTTACG	40	4.1244675E-7	38.500004	1
GCGGAAT	340	0.0	38.176468	8
>>END_MODULE