FastQCFastQC Report
Thu 26 May 2016
SRR1546109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546109_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences621614
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146212.352102751868523No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT9270.14912791539444092No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT8720.14027998082411272No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT6370.1024751694781649No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCA254.4404747E-544.011
ACGTTAG254.4404747E-544.01
TAGCGGA408.301868E-944.03
CCGTGAT207.8536896E-444.038
ATTGTCG207.8536896E-444.014
TTGTACG302.5258341E-644.01
ACGGGTC408.301868E-944.05
ACGGGTA502.7284841E-1144.05
TCTAGCG207.8536896E-444.01
ATCGTAG207.8536896E-444.01
GTAATAC207.8536896E-444.038
AATGCGT207.8536896E-444.038
ACGATAT207.8536896E-444.043
ACACTAG207.8536896E-444.035
GCTACGA254.4404747E-544.010
CCAACTA207.8536896E-444.013
ATACGAG302.5258341E-644.01
AATACGG207.8536896E-444.02
AAGCTAG207.8536896E-444.01
CGTTTTT93400.043.246251
Produced by FastQC (version 0.11.5)