##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546109_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	621614
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.156511918972225	31.0	28.0	33.0	25.0	33.0
2	28.91050233746344	31.0	28.0	33.0	16.0	33.0
3	28.344339091461904	31.0	28.0	33.0	16.0	34.0
4	32.1339142940796	35.0	32.0	35.0	25.0	37.0
5	33.730873178532015	35.0	33.0	35.0	30.0	37.0
6	33.55394666143298	35.0	33.0	37.0	28.0	37.0
7	34.2458101008021	35.0	33.0	37.0	30.0	37.0
8	34.67390534962211	35.0	35.0	37.0	32.0	37.0
9	36.46681059306901	39.0	37.0	39.0	32.0	39.0
10	36.27106049735045	38.0	35.0	39.0	31.0	39.0
11	36.386694958607755	38.0	35.0	39.0	32.0	39.0
12	35.134575476099315	37.0	34.0	39.0	29.0	39.0
13	35.255441479760755	37.0	34.0	39.0	27.0	39.0
14	35.97766137828299	38.0	34.0	40.0	27.0	41.0
15	36.56162505992465	38.0	35.0	40.0	31.0	41.0
16	36.18613802134443	38.0	34.0	40.0	31.0	41.0
17	35.70014510612695	37.0	34.0	40.0	29.0	41.0
18	35.365122085409915	37.0	34.0	39.0	29.0	40.0
19	34.82029201401513	37.0	33.0	39.0	27.0	40.0
20	35.09769567609481	35.0	34.0	39.0	29.0	40.0
21	35.31144568816018	35.0	34.0	39.0	30.0	40.0
22	35.60509576682636	36.0	34.0	39.0	31.0	40.0
23	35.9812359438494	36.0	35.0	40.0	31.0	41.0
24	35.64642527356205	35.0	34.0	40.0	31.0	41.0
25	35.33980573153114	35.0	34.0	40.0	30.0	41.0
26	35.394579272667606	35.0	34.0	40.0	30.0	41.0
27	35.66536307097331	36.0	34.0	40.0	31.0	41.0
28	35.59185764799377	36.0	35.0	40.0	31.0	41.0
29	35.716944920802945	36.0	35.0	40.0	31.0	41.0
30	35.28810644547903	36.0	34.0	40.0	30.0	41.0
31	34.59304166251082	35.0	34.0	39.0	27.0	41.0
32	34.423095361430086	35.0	34.0	40.0	25.0	41.0
33	33.99511915754793	35.0	33.0	40.0	23.0	41.0
34	33.58659875742824	35.0	33.0	40.0	19.0	41.0
35	33.23698468824705	35.0	33.0	40.0	16.0	41.0
36	32.80620771089454	35.0	33.0	40.0	15.0	41.0
37	32.71459619635336	35.0	32.0	40.0	15.0	41.0
38	32.565691892396245	35.0	32.0	40.0	13.0	41.0
39	32.39416905024662	35.0	32.0	39.0	12.0	40.0
40	32.189363495674165	35.0	31.0	39.0	10.0	40.0
41	31.878226680866263	35.0	31.0	39.0	10.0	40.0
42	32.04330018307181	35.0	31.0	39.0	10.0	40.0
43	31.804685866148446	35.0	31.0	39.0	10.0	40.0
44	31.61542532825837	35.0	31.0	39.0	10.0	40.0
45	31.52632984456592	35.0	30.0	39.0	10.0	40.0
46	31.334083530937207	35.0	30.0	38.0	10.0	40.0
47	31.29305646269228	35.0	30.0	38.0	10.0	40.0
48	31.327531233209033	35.0	30.0	38.0	10.0	40.0
49	31.33683926037702	35.0	30.0	38.0	10.0	40.0
50	31.4611205667826	35.0	30.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	1.0
12	1.0
13	8.0
14	30.0
15	74.0
16	182.0
17	426.0
18	803.0
19	1401.0
20	2174.0
21	3294.0
22	4621.0
23	6489.0
24	9557.0
25	14290.0
26	20199.0
27	22442.0
28	20862.0
29	19421.0
30	20625.0
31	24802.0
32	32190.0
33	48365.0
34	76920.0
35	58681.0
36	56341.0
37	76171.0
38	83545.0
39	17696.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.442477164285233	21.84555045414035	26.606221867589852	26.10575051398456
2	39.84369721402671	19.327428275424943	28.143188538224685	12.68568597232366
3	27.10428014813051	17.942163464786827	42.26851390090957	12.685042486173092
4	24.776629869983623	23.02409533890807	38.293860820380495	13.905413970727814
5	18.22031035337042	27.977651725990725	39.711139067009434	14.090898853629422
6	17.41820486668576	35.052942823038094	37.474863822243385	10.053988488032767
7	72.22938994295495	5.158828469114273	19.84060848050398	2.771173107426795
8	73.68785130322033	3.7425154517111903	19.39628129353586	3.1733519515326236
9	70.36054529016398	4.9706087700727455	20.623570254209202	4.045275685554057
10	42.25580504943582	20.030276023384285	25.998770941452413	11.715147985727477
11	36.83186028628699	19.336115338457628	29.943501915980015	13.888522459275372
12	32.9635432921395	18.350455427323066	35.274462930371584	13.411538350165857
13	19.619410116245774	31.109498820811627	35.318219988610295	13.952871074332302
14	14.032502485465256	34.870031884738765	37.19124730137995	13.906218328416026
15	12.71866463754034	21.504985408951534	50.9417419813582	14.834607972149918
16	14.810155498428285	15.870620674566533	51.454922186437244	17.86430164056794
17	15.599873876714486	16.418227388701027	37.76620217691365	30.21569655767084
18	18.846583249412014	21.261425900961047	41.71511581141995	18.17687503820699
19	24.4823958276358	22.804827433101572	34.47364441598806	18.239132323274575
20	25.547687149903314	20.757576245065266	34.07452213109743	19.620214473933984
21	20.374862857014158	23.475983488145378	37.68110113350085	18.468052521339608
22	19.25970135807752	20.339471118732845	34.98264196108839	25.418185562101243
23	17.185584623254947	26.0693935464774	34.605237333779485	22.139784496488176
24	17.33085162174597	21.912955628412487	44.54339831470977	16.21279443513177
25	18.075847712567604	20.5365387523447	41.84011299616804	19.547500538919653
26	15.509624944097142	26.821789728030577	36.55435688385397	21.114228444018313
27	15.482759397310872	27.291695489483825	38.849671982934744	18.375873130270552
28	13.956731991235719	24.234492788128968	44.980968897096915	16.827806323538404
29	15.246439108514288	22.08203161447458	43.29632215490642	19.375207122104715
30	17.675438455375843	24.75491221240191	40.889201337164224	16.680447995058024
31	26.011640664463798	22.68899992599909	33.46272767344365	17.836631736093462
32	25.919783016470028	22.833784309877192	33.98716888615765	17.259263787495133
33	24.029542449172638	22.853410637469555	32.9760912720756	20.14095564128221
34	18.351259785011276	24.024716303043366	34.133883728487454	23.490140183457903
35	18.98300231333271	22.726322122732114	36.099412175401454	22.19126338853372
36	27.215924995254287	23.133005369891926	31.182534498901248	18.468535135952536
37	19.054268404508264	28.575772102944914	32.29866122706374	20.07129826548308
38	20.051671937890717	29.88269247475121	28.95478544563025	21.110850141727823
39	19.188274395364328	26.87905999543125	30.810116889259255	23.122548719945176
40	21.783614912148053	23.550949624686705	31.991879204779817	22.67355625838543
41	17.850305816793057	21.9084512253585	33.13052794821223	27.110715009636206
42	21.102002207157497	24.178348621491793	28.546654354631652	26.172994816719058
43	20.757415373527625	23.56719764998858	29.893953482386177	25.781433494097623
44	19.204844163741484	26.579999806954152	32.24541274810413	21.96974328120023
45	18.891144665338942	31.453763911366217	28.00628685969106	21.64880456360378
46	22.16906311633908	27.563890131174652	29.750938685422142	20.516108067064128
47	21.550512054104313	25.254900951394273	30.307554205664612	22.8870327888368
48	20.123259772141555	24.553340175736068	33.427979421312905	21.895420630809472
49	19.927318239293196	23.630581035819656	34.1591405598973	22.28296016498985
50	19.274984154153543	27.247455816632186	32.48237652305128	20.99518350616299
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	634.0
1	918.5
2	1203.0
3	11224.5
4	21246.0
5	14890.0
6	8534.0
7	8698.0
8	8862.0
9	9437.5
10	10013.0
11	9955.0
12	9897.0
13	9413.0
14	8929.0
15	8273.5
16	7618.0
17	6920.5
18	6223.0
19	5723.0
20	5223.0
21	4867.0
22	4511.0
23	4509.5
24	4508.0
25	4270.5
26	4033.0
27	4382.0
28	4731.0
29	5149.0
30	5567.0
31	6655.0
32	7743.0
33	9284.5
34	10826.0
35	12440.0
36	14054.0
37	15078.5
38	16103.0
39	20487.0
40	24871.0
41	31279.0
42	37687.0
43	45939.5
44	54192.0
45	53621.5
46	53051.0
47	52608.5
48	52166.0
49	52300.0
50	52434.0
51	48220.0
52	44006.0
53	38412.0
54	32818.0
55	29554.5
56	26291.0
57	23632.5
58	20974.0
59	19011.0
60	17048.0
61	15165.5
62	13283.0
63	11898.0
64	10513.0
65	8880.0
66	7247.0
67	6211.0
68	5175.0
69	4272.5
70	3370.0
71	2942.5
72	2515.0
73	2136.5
74	1758.0
75	1254.5
76	751.0
77	646.5
78	542.0
79	386.0
80	230.0
81	174.5
82	119.0
83	90.5
84	62.0
85	45.5
86	29.0
87	19.0
88	9.0
89	7.5
90	6.0
91	5.0
92	4.0
93	2.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	621614.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.0885472190845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.09110979600757	25.058302640799006
2	7.369829345105069	4.729742338739971
3	3.2412912990393004	3.120249867000909
4	2.034223545892594	2.61101113226192
5	1.31396905248889	2.108167899260273
6	0.941432598643608	1.8125522637096498
7	0.7236094214326613	1.6253702561471455
8	0.5655510912599718	1.4518170317360295
9	0.4251430430767388	1.2278000351369884
>10	4.397089173879593	31.743881085081398
>50	0.8087361896488513	17.416171932883543
>100	0.08550071656733511	4.246760396995968
>500	0.0020117815662902376	0.48852108085835666
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	5.029453915725594E-4	2.3596520393888447
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14621	2.352102751868523	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	927	0.14912791539444092	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	872	0.14027998082411272	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	637	0.1024751694781649	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.050674534357334294	0.0
2	0.0	0.0	0.0	0.20269813742933718	0.0
3	0.0	0.0	0.0	0.263346707120496	0.0
4	0.0	0.0	0.0	0.36212183123288727	0.0
5	0.0	0.0	0.0	0.6217684929876097	0.0
6	0.0	0.0	0.0	0.8479538749127272	0.0
7	0.0	0.0	0.0	1.0023905510493651	0.0
8	0.0	0.0	0.0	1.3875170121651057	0.0
9	0.0	0.0	0.0	1.5733236381419982	0.0
10	0.0	0.0	0.0	1.957645741569527	0.0
11	0.0	0.0	0.0	2.3014282175111886	0.0
12	0.0	0.0	0.0	2.6191495043547923	0.0
13	1.6087153764233109E-4	0.0	0.0	2.732081323779709	0.0
14	1.6087153764233109E-4	0.0	0.0	2.7755166389431385	0.0
15	3.2174307528466217E-4	0.0	0.0	2.8430826847529174	0.0
16	3.2174307528466217E-4	0.0	0.0	2.986258353254592	0.0
17	3.2174307528466217E-4	0.0	0.0	3.15935612775774	0.0
18	3.2174307528466217E-4	0.0	0.0	3.4305855402227103	0.0
19	4.8261461292699326E-4	0.0	0.0	3.53129112278681	0.0
20	4.8261461292699326E-4	0.0	0.0	3.641648997609449	0.0
21	4.8261461292699326E-4	0.0	0.0	3.766002696206971	0.0
22	4.8261461292699326E-4	0.0	0.0	3.9029043747405945	0.0
23	4.8261461292699326E-4	0.0	0.0	4.056536693189021	0.0
24	4.8261461292699326E-4	0.0	0.0	4.1720424572162145	0.0
25	4.8261461292699326E-4	0.0	0.0	4.255695656790227	0.0
26	4.8261461292699326E-4	0.0	0.0	4.3419228009665165	0.0
27	4.8261461292699326E-4	0.0	0.0	4.444237098907038	0.0
28	4.8261461292699326E-4	0.0	0.0	4.545425296084065	0.0
29	4.8261461292699326E-4	0.0	0.0	4.658678858584266	0.0
30	4.8261461292699326E-4	0.0	0.0	4.784158657945285	0.0
31	4.8261461292699326E-4	0.0	0.0	4.910442814994514	0.0
32	4.8261461292699326E-4	0.0	0.0	5.022087662118292	0.0
33	4.8261461292699326E-4	0.0	0.0	5.1404891138230475	0.0
34	6.434861505693243E-4	0.0	0.0	5.2600166662913	0.0
35	6.434861505693243E-4	0.0	0.0	5.420727332395988	0.0
36	8.043576882116554E-4	0.0	0.0	5.541702728703021	0.0
37	9.652292258539865E-4	0.0	0.0	5.650612759686879	0.0
38	9.652292258539865E-4	0.0	0.0	5.764348936800007	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCA	25	4.4404747E-5	44.0	11
ACGTTAG	25	4.4404747E-5	44.0	1
TAGCGGA	40	8.301868E-9	44.0	3
CCGTGAT	20	7.8536896E-4	44.0	38
ATTGTCG	20	7.8536896E-4	44.0	14
TTGTACG	30	2.5258341E-6	44.0	1
ACGGGTC	40	8.301868E-9	44.0	5
ACGGGTA	50	2.7284841E-11	44.0	5
TCTAGCG	20	7.8536896E-4	44.0	1
ATCGTAG	20	7.8536896E-4	44.0	1
GTAATAC	20	7.8536896E-4	44.0	38
AATGCGT	20	7.8536896E-4	44.0	38
ACGATAT	20	7.8536896E-4	44.0	43
ACACTAG	20	7.8536896E-4	44.0	35
GCTACGA	25	4.4404747E-5	44.0	10
CCAACTA	20	7.8536896E-4	44.0	13
ATACGAG	30	2.5258341E-6	44.0	1
AATACGG	20	7.8536896E-4	44.0	2
AAGCTAG	20	7.8536896E-4	44.0	1
CGTTTTT	9340	0.0	43.24625	1
>>END_MODULE