FastQCFastQC Report
Thu 26 May 2016
SRR1546108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546108_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences625779
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151602.422580495670197No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT9410.15037257562174505No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT8780.14030512369382803No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT408.301868E-944.025
GTATTCG207.853729E-444.013
ACGTTAG302.5258614E-644.01
GACGTAG207.853729E-444.01
GTTAGTC207.853729E-444.038
TTCGTAC302.5258614E-644.033
CCGCTCG207.853729E-444.019
ACGGGTA454.783942E-1044.05
GTAGCCG207.853729E-444.043
TACGCGG254.4405082E-544.02
CTACGGG600.044.03
CGGATAC254.4405082E-544.038
CGCAGAT207.853729E-444.017
CGTACAT302.5258614E-644.035
CCGGTCA207.853729E-444.037
CGGTCTA1250.044.031
ACTCACG207.853729E-444.010
CCCGGTC207.853729E-444.036
ATGTTCG207.853729E-444.01
CGTTTTT94200.043.5796171
Produced by FastQC (version 0.11.5)