FastQCFastQC Report
Thu 26 May 2016
SRR1546107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546107_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences618712
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152782.46932336854627No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT9260.1496657572505463No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT8750.1414228267756242No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGA207.8536593E-444.00000414
ACGTTAG207.8536593E-444.0000041
GCGCGTC207.8536593E-444.00000438
CCTACTA207.8536593E-444.00000434
GAATCCG207.8536593E-444.00000413
ATTGTCG207.8536593E-444.00000414
TGCGGAT207.8536593E-444.00000413
TTATACG207.8536593E-444.0000041
CGCGTCA207.8536593E-444.00000439
TCACGAC850.044.025
AACGTAA254.4404485E-544.015
TAGCGGA454.783942E-1044.03
ATAGCGG454.783942E-1044.02
ACGGGTA351.4446414E-744.05
ATCGTAG254.4404485E-544.01
ACGTAAC254.4404485E-544.016
CGTTTTT94650.043.3724251
CGGTCTA950.041.6842131
CTACGGG900.041.5555573
AGTTATG850.041.4117661
Produced by FastQC (version 0.11.5)