##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546107_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	618712
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.007269941426706	31.0	28.0	31.0	25.0	33.0
2	28.76394671511139	31.0	28.0	33.0	16.0	33.0
3	28.22558799570721	31.0	27.0	33.0	16.0	34.0
4	32.00083722313451	35.0	32.0	35.0	25.0	37.0
5	33.692323730588704	35.0	33.0	35.0	30.0	37.0
6	33.569825702426975	35.0	33.0	37.0	28.0	37.0
7	34.203569673773906	35.0	33.0	37.0	30.0	37.0
8	34.67491013589522	35.0	35.0	37.0	32.0	37.0
9	36.53183710676373	39.0	37.0	39.0	32.0	39.0
10	36.330045319955005	38.0	35.0	39.0	32.0	39.0
11	36.43180995358099	39.0	35.0	39.0	32.0	39.0
12	35.234566324881364	37.0	34.0	39.0	30.0	39.0
13	35.31847289207256	37.0	34.0	39.0	29.0	39.0
14	36.03890986436339	38.0	34.0	40.0	29.0	41.0
15	36.564779735967626	38.0	35.0	40.0	31.0	41.0
16	36.19074625997233	38.0	34.0	40.0	31.0	41.0
17	35.69661813574005	37.0	34.0	40.0	29.0	41.0
18	35.37179495468005	37.0	34.0	39.0	29.0	40.0
19	34.78822941853399	37.0	33.0	39.0	27.0	40.0
20	35.15524670606033	35.0	34.0	39.0	30.0	40.0
21	35.28723865061612	35.0	34.0	39.0	30.0	40.0
22	35.58940347043536	36.0	34.0	39.0	31.0	40.0
23	35.97151825081783	36.0	35.0	40.0	31.0	41.0
24	35.549210294935286	35.0	34.0	40.0	31.0	41.0
25	35.2726923027192	35.0	34.0	39.0	30.0	41.0
26	35.368053310748785	35.0	34.0	40.0	30.0	41.0
27	35.67936939965606	36.0	34.0	40.0	31.0	41.0
28	35.60085306249111	36.0	35.0	40.0	31.0	41.0
29	35.70557060474017	36.0	35.0	40.0	31.0	41.0
30	35.313391367873905	36.0	34.0	40.0	30.0	41.0
31	34.57657035906852	35.0	34.0	39.0	27.0	41.0
32	34.46237829555593	35.0	34.0	40.0	25.0	41.0
33	34.05937657585436	35.0	33.0	40.0	23.0	41.0
34	33.58269922031575	35.0	33.0	40.0	18.0	41.0
35	33.242578130050816	35.0	33.0	40.0	16.0	41.0
36	32.8491705349177	35.0	33.0	40.0	15.0	41.0
37	32.765011831029625	35.0	33.0	40.0	15.0	41.0
38	32.61884204605697	35.0	32.0	40.0	13.0	41.0
39	32.380504984548544	35.0	32.0	39.0	12.0	40.0
40	32.20582920648056	35.0	31.0	39.0	10.0	40.0
41	31.877442170185805	35.0	31.0	39.0	10.0	40.0
42	32.081205795265	35.0	31.0	39.0	10.0	40.0
43	31.86313341263787	35.0	31.0	39.0	10.0	40.0
44	31.656321519543827	35.0	31.0	39.0	10.0	40.0
45	31.58123327169992	35.0	30.0	39.0	10.0	40.0
46	31.391720218777074	35.0	30.0	38.0	10.0	40.0
47	31.377351659576668	35.0	30.0	38.0	10.0	40.0
48	31.392699672868797	35.0	30.0	38.0	10.0	40.0
49	31.464316192347976	35.0	31.0	38.0	10.0	40.0
50	31.53581149226134	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	7.0
13	15.0
14	27.0
15	81.0
16	186.0
17	386.0
18	805.0
19	1355.0
20	2112.0
21	3283.0
22	4525.0
23	6550.0
24	9423.0
25	14327.0
26	19935.0
27	22230.0
28	20774.0
29	19550.0
30	20541.0
31	24081.0
32	31891.0
33	48196.0
34	76886.0
35	58350.0
36	56189.0
37	76028.0
38	83002.0
39	17975.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.526577793868555	21.771518897322178	26.38998435459471	26.311918954214562
2	39.99195102083037	19.28554804173832	27.99072912760703	12.73177180982428
3	27.082552140575906	17.84756073908377	42.35217678014973	12.717710340190589
4	24.558437528284564	23.243447678402877	38.24073236012878	13.957382433183776
5	18.190208045100142	27.832012309442845	39.864912915863925	14.11286672959309
6	17.4485059284449	34.90735592650538	37.68619325308059	9.957944891969122
7	71.92231603718693	5.297456651883267	19.97666119292983	2.8035661179999742
8	73.38988091389855	3.8104966446424187	19.59102134757367	3.208601093885362
9	70.10466905442274	5.064876711620269	20.77202317071594	4.058431063241056
10	42.13301180516945	19.96583224505101	26.167263605684067	11.733892344095477
11	36.871436144765255	19.160287823737054	30.040147920195505	13.92812811130219
12	32.98093458668977	18.315306637013666	35.34536262429046	13.358396152006103
13	19.516511721123884	31.180581595314138	35.28297495442144	14.019931729140536
14	14.049347677109866	34.81312791735088	37.30475568600577	13.832768719533483
15	12.785108418779659	21.421760043445094	50.95165440463414	14.841477133141106
16	14.827415663507415	15.840972859747346	51.54207450316141	17.789536973583832
17	15.608069667308861	16.343953244805338	37.86608308873919	30.181893999146613
18	18.82782296124853	21.23508191210127	41.791334255679544	18.145760870970662
19	24.411681040613402	22.775055276122007	34.56955740312132	18.243706280143265
20	25.405681480236364	20.71060525737338	34.29091402785141	19.592799234538848
21	20.307833046716404	23.474734609963924	37.86333544524755	18.354096898072125
22	19.216533702271814	20.32658167289466	35.1444613972252	25.31242322760832
23	17.07369503096756	26.045720787700898	34.84690776968929	22.03367641164225
24	17.3453884844645	21.74856799286259	44.68444122628945	16.221602296383455
25	17.98591267019227	20.605709926427803	42.03652103078654	19.37185637259339
26	15.510124258136257	26.688669364744825	36.66875703073482	21.132449346384103
27	15.374843222694889	27.255007176198298	38.991323911609925	18.378825689496892
28	13.86929621536353	24.251509587659527	45.135216385006274	16.743977811970677
29	15.25540154385239	22.116428968566957	43.27409198463906	19.354077502941593
30	17.556957033320835	24.648624885245475	40.939079895007694	16.855338186425996
31	25.903328204398818	22.678726127826838	33.62695405940082	17.790991608373524
32	25.866154204217796	22.729799971553806	34.24016343629992	17.16388238792847
33	23.90821577729218	22.946863807393424	33.0567372218415	20.08818319347289
34	18.32759021968218	24.229690065814143	34.21624277531387	23.2264769391898
35	18.915101048630056	22.81594667632113	36.17709047181887	22.091861803229936
36	27.029538783796013	23.212738721731597	31.234403082532747	18.523319411939642
37	19.01256157953943	28.605069887120337	32.38922147946056	19.993147053879675
38	20.09723425438653	29.840054823568963	29.09091790687751	20.97179301516699
39	19.215563945745355	26.779018347793482	30.97111418559847	23.034303520862697
40	21.830674045436325	23.700364628453947	32.000349112349525	22.468612213760196
41	17.818306417202187	22.114489455514036	33.20591810082882	26.861286026454955
42	21.038221337229597	24.26977333557455	28.620585991543724	26.07141933565213
43	20.810490179598908	23.586579862682473	29.951415198024282	25.651514759694333
44	19.138629927979416	26.658283660249033	32.20787700901227	21.99520940275928
45	18.8536831352875	31.595475762551885	28.124232276083216	21.426608826077402
46	22.149239064378904	27.593613830021074	30.089282250869548	20.16786485473047
47	21.493360400315495	25.352506497368726	30.393462548002947	22.76067055431283
48	20.082041402138636	24.645069111315117	33.39308111043587	21.879808376110372
49	19.897787662110964	23.70440528064754	34.246143601546436	22.151663455695054
50	19.325954563674212	27.242400341354294	32.63020597628622	20.80143911868527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	581.0
1	905.0
2	1229.0
3	11740.5
4	22252.0
5	15432.5
6	8613.0
7	8853.0
8	9093.0
9	9526.0
10	9959.0
11	9908.5
12	9858.0
13	9299.0
14	8740.0
15	8068.5
16	7397.0
17	6754.5
18	6112.0
19	5651.0
20	5190.0
21	4764.5
22	4339.0
23	4386.0
24	4433.0
25	4189.0
26	3945.0
27	4336.5
28	4728.0
29	5132.0
30	5536.0
31	6599.0
32	7662.0
33	9359.5
34	11057.0
35	12606.5
36	14156.0
37	15156.5
38	16157.0
39	20509.0
40	24861.0
41	31481.0
42	38101.0
43	46112.0
44	54123.0
45	53346.0
46	52569.0
47	52028.5
48	51488.0
49	51422.5
50	51357.0
51	47750.5
52	44144.0
53	38241.0
54	32338.0
55	29164.0
56	25990.0
57	23233.0
58	20476.0
59	18683.5
60	16891.0
61	15057.0
62	13223.0
63	11875.5
64	10528.0
65	8751.0
66	6974.0
67	6052.5
68	5131.0
69	4268.0
70	3405.0
71	3008.0
72	2611.0
73	2181.0
74	1751.0
75	1276.0
76	801.0
77	649.0
78	497.0
79	346.0
80	195.0
81	152.0
82	109.0
83	85.0
84	61.0
85	41.5
86	22.0
87	18.0
88	14.0
89	10.0
90	6.0
91	6.0
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	618712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.63558521500123
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.99463126335144	24.67405803644354
2	7.207669396828085	4.560376788098228
3	3.1674300342743344	3.006105082855199
4	1.9560917387279078	2.4752842755554663
5	1.3829829816211936	2.1875737982986876
6	1.0722352061218146	2.0352472940274646
7	0.7670153204418204	1.6985484971734082
8	0.5918330325036649	1.497838746625783
9	0.47690236372023365	1.3578376830036267
>10	4.4642067535325	31.77967360586728
>50	0.8296687624275422	17.510814921950928
>100	0.08676610201128784	4.250691363877335
>500	0.002053635550563026	0.4844985800483776
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	5.134088876407565E-4	2.481451326174694
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15278	2.46932336854627	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	926	0.1496657572505463	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	875	0.1414228267756242	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6162608774357052E-4	0.0	0.0	0.05285173069214756	0.0
2	1.6162608774357052E-4	0.0	0.0	0.19427455746777175	0.0
3	1.6162608774357052E-4	0.0	0.0	0.24906580121284216	0.0
4	1.6162608774357052E-4	0.0	0.0	0.34054616687570305	0.0
5	1.6162608774357052E-4	0.0	0.0	0.5938142463698781	0.0
6	1.6162608774357052E-4	0.0	0.0	0.8133024735256468	0.0
7	1.6162608774357052E-4	0.0	0.0	0.966039126443321	0.0
8	1.6162608774357052E-4	0.0	0.0	1.3292129456031239	0.0
9	1.6162608774357052E-4	0.0	0.0	1.5144364421572558	0.0
10	3.2325217548714104E-4	0.0	0.0	1.8734079830357258	0.0
11	3.2325217548714104E-4	0.0	0.0	2.192134628066047	0.0
12	3.2325217548714104E-4	0.0	0.0	2.485970855583858	0.0
13	4.8487826323071154E-4	0.0	0.0	2.592159195231384	0.0
14	4.8487826323071154E-4	0.0	0.0	2.638545882413789	0.0
15	4.8487826323071154E-4	0.0	0.0	2.6920441174569105	0.0
16	4.8487826323071154E-4	0.0	0.0	2.820860109388536	0.0
17	4.8487826323071154E-4	0.0	0.0	2.9836175797463116	0.0
18	4.8487826323071154E-4	0.0	0.0	3.248037859294793	0.0
19	6.465043509742821E-4	0.0	0.0	3.342912372800269	0.0
20	8.081304387178525E-4	0.0	0.0	3.455404129869794	0.0
21	8.081304387178525E-4	0.0	0.0	3.5861596348543427	0.0
22	8.081304387178525E-4	0.0	0.0	3.7191779050673013	0.0
23	8.081304387178525E-4	0.0	0.0	3.8696517927565655	0.0
24	8.081304387178525E-4	0.0	0.0	3.973738993263425	0.0
25	8.081304387178525E-4	0.0	0.0	4.050673011029364	0.0
26	8.081304387178525E-4	0.0	0.0	4.139405733200585	0.0
27	8.081304387178525E-4	0.0	0.0	4.238159272811906	0.0
28	8.081304387178525E-4	0.0	0.0	4.3322256558786645	0.0
29	8.081304387178525E-4	0.0	0.0	4.4350198476835745	0.0
30	8.081304387178525E-4	0.0	0.0	4.568038117896533	0.0
31	8.081304387178525E-4	0.0	0.0	4.684085648896417	0.0
32	8.081304387178525E-4	0.0	0.0	4.77928341457738	0.0
33	8.081304387178525E-4	0.0	0.0	4.903897128227673	0.0
34	8.081304387178525E-4	0.0	0.0	5.007176198295815	0.0
35	9.697565264614231E-4	0.0	0.0	5.167024399074206	0.0
36	0.0011313826142049935	0.0	0.0	5.275798756125629	0.0
37	0.0011313826142049935	0.0	0.0	5.3781080696673085	0.0
38	0.0011313826142049935	0.0	0.0	5.49447885284268	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGA	20	7.8536593E-4	44.000004	14
ACGTTAG	20	7.8536593E-4	44.000004	1
GCGCGTC	20	7.8536593E-4	44.000004	38
CCTACTA	20	7.8536593E-4	44.000004	34
GAATCCG	20	7.8536593E-4	44.000004	13
ATTGTCG	20	7.8536593E-4	44.000004	14
TGCGGAT	20	7.8536593E-4	44.000004	13
TTATACG	20	7.8536593E-4	44.000004	1
CGCGTCA	20	7.8536593E-4	44.000004	39
TCACGAC	85	0.0	44.0	25
AACGTAA	25	4.4404485E-5	44.0	15
TAGCGGA	45	4.783942E-10	44.0	3
ATAGCGG	45	4.783942E-10	44.0	2
ACGGGTA	35	1.4446414E-7	44.0	5
ATCGTAG	25	4.4404485E-5	44.0	1
ACGTAAC	25	4.4404485E-5	44.0	16
CGTTTTT	9465	0.0	43.372425	1
CGGTCTA	95	0.0	41.68421	31
CTACGGG	90	0.0	41.555557	3
AGTTATG	85	0.0	41.411766	1
>>END_MODULE