FastQCFastQC Report
Thu 26 May 2016
SRR1546106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546106_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences524101
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77651.4815846563925654No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT5660.10799445145115158No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT5270.10055313765858108No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA207.852555E-444.00000430
ACACACG207.852555E-444.00000428
ACGTCTT207.852555E-444.00000431
CACCGTG207.852555E-444.00000418
TACGTTG207.852555E-444.0000041
TACCGAA207.852555E-444.00000418
CCGATGT254.4395158E-544.011
CTATCGA254.4395158E-544.038
GGCCGTA302.525072E-644.08
ACGGGAC1050.044.05
ATCGTAG254.4395158E-544.01
CGGGATA254.4395158E-544.06
TAGGACG254.4395158E-544.01
ACGGGTA454.783942E-1043.9999965
CGTTTTT83450.043.340921
GCTTAAA1300.042.3076922
CGTCTTT1050.041.9047621
GACGGTC900.041.55555329
CGGTCTA900.041.55555331
CTAGGGT850.041.4117664
Produced by FastQC (version 0.11.5)